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Poster: Virus clearance using Eshmuno HCX
150 mM NaCl
75 mM NaCl
0 mM NaCl
300 mM NaCl
150 mM NaCl
75 mM NaCl
0 mM NaCl
300 mM NaCl
150 mM NaCl
75 mM NaCl
Evaluation of MDCK Cell Growth and Virus Production in EX-CELL MDCK
resuspended in 20 mL
fresh medium and plated into 75 cm2 T-fl asks.
0.00E+00
5.00E+04
1.00E+05
1.50E+05
2.00E+05
2.50E+05
3.00E+05
3.50E+05
4.00E+05
4.50E+05
5.00E+05
Thaw (Day 0) 1 (Day 3) 2 (Day 6)
Passage
Milliflip™Application Note
lo
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75
%
La
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50
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S
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05
Product Information Sheet-rdlig-ro
https://www.sigmaaldrich.com
2. How to Use this Product
y Version: 05 Rapid DNA Dephos & Ligation Kit
8 sigma-aldrich.com
For the ligation, set up the following reaction:
Reagent
B6-WHITE™ MURINE ES CELL LINE
Inject 8-12 cells/C57BL/6 blastocyst.
Day 0
Prepare the following culture dishes containing a monolayer of mitotically inactivated PMEF
feeder cells:
• 1 x 25 cm2 flask
• 1 x 75
The Vmax Nomograph
10
0.
05
0
0.
02
5
0.
01
0
0.
00
50
0.
00
15
0.
00
07
5
0.
00
05
0.
00
03
5
0.
00
02
5
0.
00
Product Information Sheet-04716728001
minutes.
Stop the reaction by adding 2 µl 0.2 M EDTA, pH 8.0 to a final concentration of 8 mM.
– Heat to +75°C for 10 minutes.
The concentration of EDTA must be considered for
MBP, recombinant
Catalog # 05-429, 1 μg/mL).
Proteins were visualized using a
goat anti-mouse secondary
antibody conjugated to HRP and a
chemiluminescence detection
system.
250
150
100
75
Glass Fiber Filters
50
124 50 APFF 124 50
Type AP40 10 100 AP40 010 00
24 500 AP40 024 05
37 500 AP40 037 05
47 500 AP40 047 05
90 100 AP40 090 05
142 50 AP40 142 50
Â
Millipore Worldwide
AUSTRALIA
Tel.
Anti-Fodrin, alpha, clone AA6 - Data Sheet
Am. J. Resp. Cell Mol. Biol. (1994) 11:75-84.
5. Differentiation (1993) 54:35-45.
6. J. Bio. Chem. (1995). 270:6425-6428
7. Amer. J. Physiol. (1996) 271:F292.
8. Molecular Brain Research. (1998) 54:
Anti-BACE 1 C-Terminus (485-501) antibody produced in rabbit (B0806)
Chem., 276, 4211
(2001).
16. Walter, J., et al., J. Biol. Chem., 276, 14634 (2001).
ER/KAA 05/05
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
Polygard®-CR Filters
.3 μm
A5 = 0.5 μm
01 = 1.0 μm
03 = 3.0 μm
05 = 5.0 μm
10 = 10.0 μm
25 = 25.0 μm
50 = 50.0 μm
75 = 75.0 μm
99 = 100.0 μm
Capsule Length
51 = Opticap® XL 1*
05 = Opticap® XL 5
10
EX-CELL CHO Medium (C5467)
Sigma's CHO Protein-Free Medium vs Competitors'
Cell Growth and Viability
0.0E+00
2.0E+05
4.0E+05
6.0E+05
8.0E+05
1.0E+06
1.2E+06
1.4E+06
1.6E+06
0 5 10 15 20
Days in Culture
Figure 1
V
ia
Complete Solutions for Dissolution Testing
2 Caffeine 2.2 1.03 7.1
3 Aspirin 3.7 1.04 16.8
19
0,E+00
2,E+05
4,E+05
6,E+05
8,E+05
0 1 2 3 4
A
b
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rb
a
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ce
Data Sheet - I5408
2.50E+07
0 2 4 6 8 10 12 14 16
Day
V
ia
b
le
C
el
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p
er
m
L
TiterHigh Sf Competitor A
0.00E+00
1.00E-05
2.00E-05
3.00E-05
RABBIT ANTI-LIPIN-1, N-TERM
Technical Support: T: 1-800-MILLIPORE (1-800-645-5476) • F: 1-800-437-7502
www.millipore.com
05/31/06/AB3585/BH/JC
RABBIT ANTI-LIPIN-1, N-TERM
POLYCLONAL ANTIBODY
CATALOG NUMBER:
MultiScreen HTS Glass Fiber Filter Plates
Quality Results
Sc
op
ol
am
in
e
Bo
un
d
(%
o
f C
on
tro
l)
100
75
50
25
0
–9 –8 –7
Pirenzipine (Log M)
–6
Triplicate Data
Protocol- EZHPYYT66K
For research use only. Not for use in diagnostic procedures.
IFU-EZHPYYT66K Rev 05/24 8
PYY Quality Control 1 and 2 Preparation
Use care in opening the lyophilized Quality Control
Data Sheet - SAB4200694
., 176, 7566-75
(2006).
5. Filova E., et al., Int J Nanomedicine, 10, 7145-63
(2015).
6. Fath KR., et al., J. Cell Sci., 92, 67-75 (1989).
7. Yan J., et al., Cell Mol Bioeng., 8, 151-59
Quality Control Ranges: MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel
IL-8 Control 1 60 - 136 pg/mL TNFα Control 1 328 - 682 pg/mL
Control 2 382 - 794 pg/mL Control 2 1621 -3367 pg/mL
Protocol- 48-655MAG
For research use only. Not for use in diagnostic procedures.
48-655MAG Rev 05/23 8
Sample Collection and Storage
Considerations for Cell Stimulation
1. Treating cells
Flyer - Transcriptor Universal cDNA Master for qRT-PCR
Master today by calling
800 262 1640 or online at www.roche-applied-science.com
Cat. No. Product
05 943 523 001
NEW!
RealTime ready Cell
Lysis Kit
(500 x 40 µl reactions)
Kit Contents:
RealTime
Product Information Sheet-10378445001
www.sigmaaldrich.com
05
21
.1
14
93
77
90
01
17
4. Supplementary Information
y Version: 17 Chromozym PK Benzoyl-Pro-Phe-Arg-4-nitranilide acetate
8 sigma-aldrich.com
4. Supplementary
A Novel Silica-Based Immobilized Metal Affi nity Chromatography (IMAC) Technology for the Enrichment of Phosphopeptides
peptide retention.
Flow through Fractions
-5 0000
0
5 0000
1 E +05
2 E +05
2 E +05
3 E +05
3 E +05
15 16 17 18 19Time
Data Sheet - B3795
SH-SY5Y at 50 ng/mL, differentiated by retinoic
acid treatment, in a serum free medium.3
B3795Dat 05/22
Purity: ≥ 95% (SDS-PAGE)
Endotoxin: ≤ 1.00 EU/µg growth factor
Precautions and Disclaimer
Data Sheet - SRP3014
SRP3014Dat 05/22
1
Data
Data Sheet - B3796
B3796Dat 05/22 1
Data Sheet
Product Information Sheet - C4364
Testing Amino Acid Mix, Metals,
and Iron Mix 1
0.0E+00
5.0E+05
1.0E+06
1.5E+06
2.0E+06
2.5E+06
3.0E+06
3.5E+06
4.0E+06
4.5E+06
0 2 4 6 8 10 12 14
Days
V
ia
b
le
C
el
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p
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Product Information Sheet - P3428
suitable for Protein Sequencing
Catalog Number P3428
Storage Temperature 2–8 °C
TECHNICAL BULLETIN
CAS RN 72561-05-8
EC 3.4.21.50
Synonym: Lys-C
Product Description
Endoproteinase Lys-C from
Data Sheet - G4170
-101
(2003).
11. Wadhwa, R., et al., Cell Res., 9, 261-269
(1999).
KAA/EK 08/05
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