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Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Water Purification Systems

Thank you for your interest in the Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Water Purification Systems offering. For additional information on this offering or to be contacted by a Sales Representative, please complete and submit the form below.

Gold Nanorod Synthesis

Plasmonic nanoparticles have unique optical properties that can be tailored to suit a variety of applications in the biotechnology1–8 and electronics9–16 industries.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Milli-Q^® IQ 7003/05/10/15 Ultrapure & Pure Lab Water Purification System

Milli-Q® IQ 7003/05/10/15 lab water systems provide consistent-quality Type 1 and Type 2 water, are intuitive to use, and can support your lab's sustainability goals.

Determination of vitamins B and preservatives

Learn to separate and quantify B vitamins and preservatives in capsules with HPLC-UV and Purospher® STAR RP-8 column. Validated for precision.

Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection

Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus

Surfactants (nonionic) in Extran^® Rinse Solutions

Learn about the sample preparation and photometric determination of non-ionic surfactants in Extran® rinse solutions (MA 01, MA 02, MA 05, AP 22) using Spectroquant® test kits and spectrophotometers.

Determination of 11 Beta Agonist Residues in Pork by LC-MS

Using an Ascentis® Express F5 HPLC column, this study separates 11 β-agonists in pork by LC-MS/MS in under 8 minutes with excellent recovery and precision.

Determination of Eight Aminoglycosides in Pork Meat

LC/LC-MS method identifies 8 aminoglycosides in pork using Discovery® DSC-18 SPE and Ascentis® Express C18 UHPLC, per Chinese standards.

Selectivity and the Properties of a HIC Medium

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Detection of Signaling Effector-Complexes Downstream of BMP4 Using In Situ PLA, A Proximity Ligation Assay

BMPs are responsible for a wide range of developmental and biological effects. BMP receptors activate (phosphorylate) the Smad1/5/8 effectors, which then, form a complex with Smad4 and translocate to the nucleus where they function as transcription factors to initiate BMP

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

EcoVadis rating 2024 | EMD Surface Portal

We are proud of the continued recognition of our sustainability leadership. This is reflected by further improving our official EcoVadis score to 75/100 in 2024, now placing us among the top 3% of all companies, across all industries, taking part

Pressure-Temperature Calculator for Solvents

Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.

Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF

This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Peptide Stability and Potential Degradation Pathways

To prevent or minimize peptide degradation we recommend storing the peptide in lyophilized form at –20 °C or –80 °C. Once in solution, the peptide/protein should be stored frozen in individual aliquots to avoid freeze-thaw cycles. Also, prolonged exposure to

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Laboratory Water Purification Techniques & Technologies

Explore how various water purification technologies effectively remove contaminants to ensure laboratory-grade water quality for scientific analyses.

Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells

Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic ﬂuid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

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