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Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

Enzymatic Assay of 5’-Nucleotidase

To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Limited-Time Offer: Save 25% on products for Assay Development

Get 25% off for selected products for Diagnostic customers! Discover our broad portfolio to enhance your work and get the product you need at one shop.

Parvovirus Filtration Best Practices - 25 Years of Hands-on Experience

This webinar will help parvovirus filter users achieve optimized performance and trouble-free operation.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Order shRNA Online in 5 Easy Steps

5 easy steps to order shRNA gene of your interest.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

MilliporeSigma Invests $25 Million to Expand Cell Culture Media Production in Kansas

MilliporeSigma, the U.S. and Canada Life Science business of Merck KGaA, Darmstadt Germany, today announced the expansion of its facility in Lenexa, Kansas, adding 98,000 square feet of lab space and production capability to manufacture cell culture media.

ACS Fall 2022

Visit us at ACS Fall 2022 in Chicago, Illinois, August 21-25, 2022.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

OverExpress™ Electrocompetent Cells

OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Download our Training Program

Our Training Program is availabale in 5 different languages.

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Hydroxymethylfurfural Analysis of Beer by HPLC UV

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Gene Therapy Process Development

Leverage our 25+ years of expertise for robust viral vector process development ensuring quality and safety from discovery to commercialization.

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

Chiral Disulfonimides for Asymmetric Counteranion-directed Catalysis (ACDC)

Asymmetric counteranion-directed catalysis (ACDC) has a number of valuable applications in enantioselective synthesis.

Extractables Studies of Single-Use Equipment Immediate Identification and Quantification of Unknown Extractables by GC/MS with a Certified Reference Material Mix for Extractables and Leachables

Identification and quantification of the most common extractables originating from single-use equipment by GC/MS and an Extractables and Leachables Screening Standard.

NMR Deuterated Solvent Properties Reference Chart

Use this reference table to find the coupling values and chemical shifts of our NMR (deuterated) solvents. Melting and boiling points, molecular weight, density, and CAS number are also listed.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Summer Sale Promo - ELISA & Specialty Assays

It’s time to enjoy your summer, not spend time searching for research products. Now through July 29, save up to 25% off select assay products

Column Packing and Preparation for Size Exclusion Chromatography

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Reflectometric Determination of Hydroxymethylfurfural in Honey

Rapid analysis of 5-(Hydroxymethyl)furfural (HMF) in honey using a reflectometer and Reflectoquant® HMF Test.

Nicewicz Photoredox Catalysts for Anti-Markovnikov Alkene Hydrofunctionalization

Explore Nicewicz Photoredox catalysts—photoexcitable acridinium salts for efficient, metal-free anti-Markovnikov hydrofunctionalizations.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

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