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Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

MilliporeSigma Expands in Gillingham, UK, Boosts Distribution Capabilities

MilliporeSigma today announced the completion of a £9 million expansion to its Gillingham distribution center.

Accelerated mAb Development Program – From Transfection to GMP Drug Substance in 9 Months

The accelerated mAb development program delivers GMP drug substance nine months from transfection. We designed the mAbExpress™ program to be fast, cost effective, and appropriate for the early clinical phase of your program, with regulatory support throughout.

How to Boost AAV Titer with Feed Supplementation in both HEK293 and Sf-9 Platforms

In this webinar, you will learn wow to boost AAV titers in HEK293 and Sf-9 cell lines as well as getting some insider tips on volume and timing optimization.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Analysis of 17 Cannabinoids in Hemp and Cannabis

HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. Read the application note.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Materials for Clean H² Production from Bioethanol Reforming

Hydrogen is one of the most important resources in providing food, fuel, and chemical products for our everyday life. Sustainable catalytic hydrogen production from bioethanol has gained significant attention in recent years due to globally diminishing fossil fuel supplies, which

Protector RNase Inhibitor Protocol

Protector RNase Inhibitor stabilizes RNA at -80°C without permeating intact cells, unsuitable for cell pellet addition.

MilliporeSigma's Bioprocessing 4.0 Technologies

Blazar™ platform wins R&D 100 and CPhI Awards for reducing testing times by up to 80 percent.

Determination of Phosphorus Total in Meat and Meat Products

Colorimetric total phosphorus analysis method for meat and meat products as per German Food and Feed Code §64 LFGB 06.00-9.

Accelerating Protein Degrader Discovery with IAP

Plate of 80 ligands against E3 ligase IAP designed by ComInnex; allows creation of bifunctional targeted protein degraders or molecular glues.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Dissecting MOCRA Modernization Cosmetic Regulations Act

MoCRA brings the most significant changes to US cosmetic regulations in 80 years, requiring companies to monitor FDA updates and adopt new compliance policies.

Analysis of Antioxidants in Bakery Products

HPLC-UV method detects 9 antioxidants like propyl gallate in food using a Discovery®</sup HS C18 column for excellent peak shapes.

Size Exclusion Analysis of Tween-Containing IgG Formulations

In this study, we investigated the effect of adding Tween® 20 and Tween 80 on the elution of proprietary mAb 221 under size exclusion chromatography conditions.

Review of O-Linked Glycoproteins

O-Linked glycoproteins are usually large proteins with a molecular mass of >200 kDa. Glycosylation generally occurs in high-density clusters and may represent as much as 50-80% of the overall mass.

Biorepository & Storage

Store your precious material in GMP-compliant facilities amongst three continents. A range of materials including virus and cell banks can be stored in ambient, liquid nitrogen or –80 conditions.

Novel Non-Pyrophoric t- and n-Butyllithium Formulations for Chemical Synthesis

Non-pyrophoric t-BuLi and n-BuLi formulations offer robust reactivity with improved handling.

Recent Advances in Scalable Synthesis and Processing of Two-Dimensional Materials

Advances in scalable synthesis and processing of two-dimensional materials

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.

2D MXenes Ink: Synthesis, Properties, and Their Precursors

Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.

Graphene-Based Composites and their Unique Renewable Energy Applications

Graphene is a unique two-dimensional (2D) structure of monolayer carbon atoms packed into a dense honeycomb crystal that has attracted great interest due to its diverse and fascinating properties.

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

Revolution in Plastic Recycling: Laser Marking & Digital Product Passports Enable True Circular Economy

An industry-wide project demonstrates how packaging can become smarter, more sustainable, and fully traceable. Plastics are a blessing—and a global problem. Lightweight, durable, and versatile. Yet these very properties make plastics a challenge worldwide. Over 400 million tons are produced

AdQPhos and Pd pre catalysts for alpha arylation reaction

Discover AdQPhos and its Pd pre-catalysts for selective α-arylation of diverse nucleophiles under mild aqueous conditions without hazardous solvents.

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

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