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Showing 1-30 of 112 results for "856177" within Papers
Jaehyuk Choi et al.
PloS one, 10(8), e0134939-e0134939 (2015-08-05)
Rice blast fungus, Magnaporthe oryzae, is the most destructive pathogen in the rice-growing area. This fungus has a biotrophic phase early in infection and later switches to a necrotrophic lifestyle. During the biotrophic phase, the fungus competes with its host
David Virant et al.
Nature communications, 9(1), 930-930 (2018-03-04)
Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous
Alexander N Patananan et al.
Archives of biochemistry and biophysics, 569, 32-44 (2015-02-11)
l-Ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule
Claudia Montllor-Albalate et al.
Redox biology, 21, 101064-101064 (2018-12-24)
Cu/Zn Superoxide Dismutase (Sod1) is a highly conserved and abundant metalloenzyme that catalyzes the disproportionation of superoxide radicals into hydrogen peroxide and molecular oxygen. As a consequence, Sod1 serves dual roles in oxidative stress protection and redox signaling by both
Ahruem Baek et al.
Autophagy, 13(2), 248-263 (2017-01-04)
Contribution of autophagy and regulation of related proteins to the degeneration of retinal pigment epithelium (RPE) in age-related macular degeneration (AMD) remain unknown. We report that upregulation of KRT8 (keratin 8) as well as its phosphorylation are accompanied with autophagy
Laura Álvarez-Fraga et al.
Virulence, 9(1), 496-509 (2018-01-16)
Acinetobacter baumannii has emerged in the last decade as an important nosocomial pathogen. To identify genes involved in the course of a pneumonia infection, gene expression profiles were obtained from A. baumannii ATCC 17978 grown in mouse infected lungs and
Queenie Hu et al.
G3 (Bethesda, Md.), 8(12), 3857-3863 (2018-10-10)
The overproduction of reactive oxygen species (ROS) in cells can lead to the development of diseases associated with aging. We have previously shown that C. elegans BRAP-2 (Brca1 associated binding protein 2) regulates phase II detoxification genes such as gst-4
Human Riahi et al.
PLoS biology, 17(3), e2006146-e2006146 (2019-03-13)
Stress responses are crucial processes that require activation of genetic programs that protect from the stressor. Stress responses are also energy consuming and can thus be deleterious to the organism. The mechanisms coordinating energy consumption during stress response in multicellular
Pin-Chao Liao et al.
STAR protocols, 1(3), 100160-100160 (2020-12-31)
The redox state of mitochondria is one indicator of the functional state of the organelles. Mitochondria are also the primary endogenous source of reactive oxygen species (ROS). Therefore, the redox state of the organelles also reflects their function in ROS
Dong Suk Yoon et al.
Bio-protocol, 8(6) (2018-05-11)
Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum
Andrey A Parkhitko et al.
eLife, 9 (2020-12-16)
Aging is characterized by extensive metabolic reprogramming. To identify metabolic pathways associated with aging, we analyzed age-dependent changes in the metabolomes of long-lived Drosophila melanogaster. Among the metabolites that changed, levels of tyrosine were increased with age in long-lived flies.
Yan Wang et al.
Autophagy, 11(12), 2259-2274 (2015-11-15)
Microtubules, the major components of cytoskeleton, are involved in various fundamental biological processes in plants. Recent studies in mammalian cells have revealed the importance of microtubule cytoskeleton in autophagy. However, little is known about the roles of microtubules in plant
Shilpi Khare et al.
Mechanisms of ageing and development, 130(10), 670-680 (2009-08-18)
We have shown that Caenorhabditis elegans lacking the PCM-1 protein repair l-isoaspartyl methyltransferase are more sensitive to oxidative stress than wild-type nematodes. Exposure to the redox-cycling quinone juglone upon exit from dauer diapause results in defective egg-laying (Egl phenotype) in
Michael M Mueller et al.
Nature cell biology, 16(12), 1168-1179 (2014-11-25)
Genome maintenance defects cause complex disease phenotypes characterized by developmental failure, cancer susceptibility and premature ageing. It remains poorly understood how DNA damage responses function during organismal development and maintain tissue functionality when DNA damage accumulates with ageing. Here we
David J Turner et al.
The CRISPR journal, 4(3), 427-437 (2021-06-08)
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing system has been broadly adopted for high-throughput genetic screens. However, the application of genome-wide single guide RNA (sgRNA) libraries can be challenging. We generated a custom sgRNA library, an order
Vayu Maini Rekdal et al.
eLife, 9 (2020-02-19)
Catechol dehydroxylation is a central chemical transformation in the gut microbial metabolism of plant- and host-derived small molecules. However, the molecular basis for this transformation and its distribution among gut microorganisms are poorly understood. Here, we characterize a molybdenum-dependent enzyme
Piet Kramer et al.
Frontiers in genetics, 7, 165-165 (2016-09-30)
The mitochondrial permeability transition pore plays a key role in programmed cell death and the induction of autophagy. Opening of the pore is regulated by the mitochondrial peptidyl prolyl-cis, trans-isomerase cyclophilin D (CYPD). Previously it was shown in the aging
Natalia N Singh et al.
PloS one, 7(11), e49595-e49595 (2012-11-28)
Humans have two near identical copies of Survival Motor Neuron gene: SMN1 and SMN2. Loss of SMN1 coupled with the predominant skipping of SMN2 exon 7 causes spinal muscular atrophy (SMA), a neurodegenerative disease. SMA patient cells devoid of SMN1
Isaak E Müller et al.
Nature communications, 10(1), 4028-4028 (2019-09-08)
Crosstalk is a major challenge to engineering sophisticated synthetic gene networks. A common approach is to insulate signal-transduction pathways by minimizing molecular-level crosstalk between endogenous and synthetic genetic components, but this strategy can be difficult to apply in the context
Tetrahedron Letters, 33, 6677-6677 (1993)
Matthias Wiemer et al.
Microbial cell (Graz, Austria), 1(7), 225-240 (2014-06-22)
Aging of biological systems is influenced by various factors, conditions and processes. Among others, processes allowing organisms to deal with various types of stress are of key importance. In particular, oxidative stress as the result of the generation of reactive
Khadija Hajji et al.
Human molecular genetics, 28(11), 1905-1918 (2019-02-05)
Parkinson's disease (PD) is a progressive neurodegenerative movement disorder that can arise after long-term exposure to environmental oxidative stressors, such as the herbicide paraquat (PQ). Here we investigated the potential neuroprotective action of vertebrate pituitary adenylate cyclase-activating polypeptide (PACAP) against
Richard de Boer et al.
PloS one, 10(5), e0126220-e0126220 (2015-05-15)
Today HIV-1 infection is recognized as a chronic disease with obligatory lifelong treatment to keep viral titers below detectable levels. The continuous intake of antiretroviral drugs however, leads to severe and even life-threatening side effects, supposedly by the deleterious impact
Oscar M Arenas et al.
Nature neuroscience, 20(12), 1686-1693 (2017-12-01)
All animals must detect noxious stimuli to initiate protective behavior, but the evolutionary origin of nociceptive systems is not well understood. Here we show that noxious heat and irritant chemicals elicit robust escape behaviors in the planarian Schmidtea mediterranea and
Jiasheng Zhang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(3), 557-568 (2019-11-30)
Mitochondria are important sources of energy, but they are also the target of cellular stress, toxin exposure, and aging-related injury. Persistent accumulation of damaged mitochondria has been implicated in many neurodegenerative diseases. One highly conserved mechanism to clear damaged mitochondria
Aracely Garcia-Garcia et al.
Toxicological sciences : an official journal of the Society of Toxicology, 136(1), 166-182 (2013-09-03)
Controversial reports on the role of autophagy as a survival or cell death mechanism in dopaminergic cell death induced by parkinsonian toxins exist. We investigated the alterations in autophagic flux and the role of autophagy protein 5 (Atg5)-dependent autophagy in
S Dong et al.
Nature communications, 11(1), 645-645 (2020-02-02)
Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack
Teresa Z Rzezniczak et al.
G3 (Bethesda, Md.), 2(12), 1613-1623 (2013-01-01)
Interactions across biological networks are often quantified under a single set of conditions; however, cellular behaviors are dynamic and interactions can be expected to change in response to molecular context and environment. To determine the consistency of network interactions, we
Zaijun Ma et al.
eLife, 7 (2018-05-29)
Epigenetic alteration has been implicated in aging. However, the mechanism by which epigenetic change impacts aging remains to be understood. H3K27me3, a highly conserved histone modification signifying transcriptional repression, is marked and maintained by Polycomb Repressive Complexes (PRCs). Here, we
Susanna Maria Früh et al.
Nature communications, 6, 7275-7275 (2015-06-05)
Fibronectin fibrils within the extracellular matrix play central roles in physiological and pathological processes, yet many structural details about their hierarchical and molecular assembly remain unknown. Here we combine site-specific protein labelling with single-molecule localization by stepwise photobleaching or direct
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