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Showing 1-30 of 65 results for "89510" within Papers
Adi Egozi et al.
Cell reports, 32(7), 108043-108043 (2020-08-20)
The islets of Langerhans are dynamic structures that can change in size, number of cells, and molecular function in response to physiological and pathological stress. Molecular cues originating from the surrounding "peri-islet" acinar cells that could facilitate this plasticity have
Thomas Tu et al.
JHEP reports : innovation in hepatology, 3(1), 100195-100195 (2021-01-02)
Chronic HBV infection cannot be cured by current therapeutics owing to their limited ability to reduce covalently closed circular (ccc)DNA levels in the livers of infected individuals. Therefore, greater understanding of the molecular determinants of cccDNA formation and persistence is
Gustavo Hernandez-Vargas et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1105, 120-128 (2018-12-26)
Fractionation of native, mono and di-PEGylated lysozyme was performed in 36 different polymer-polymer aqueous two-phase systems using UCON as a phase-forming component. After a discrete partition analysis, dextran 75 kDa-UCON, volume ratio 3, tie-line length 35% w/w; ficoll 70 kDa-UCON, volume ratio
Ananthakrishnan Soundaram Jeevarathinam et al.
Biosensors & bioelectronics, 126, 831-837 (2019-01-04)
Heparin is an indispensable drug in anticoagulation therapy but with a narrow therapeutic window, which dictates regular testing and dose adjustment. However, current monitoring tools have a long turnaround time or are operator intensive. In this work, we describe a
Expression, purification and crystallization of the herpesvirus nuclear egress complex (NEC)
Bigalke JM and Heldwein EE
Bio-protocol, 6(14), 44708-44708 (2016)
Sujeet Kumar et al.
Bio-protocol, 9(12), e3271-e3271 (2019-06-20)
Transporters are dynamic membrane proteins that are essential to the physiology of cells. To function, transporters must cycle between various conformational states, so to understand their mechanistic details, it is critical to characterize how their structure changes during the transport
Adrian T Grzybowski et al.
Nature protocols, 14(12), 3275-3302 (2019-11-15)
Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-seq) has served as the central method for the study of histone modifications for the past decade. In ChIP-seq analyses, antibodies selectively capture nucleosomes bearing a modification of interest and the associated DNA is
Oliver J Harrison et al.
Cell reports, 30(8), 2655-2671 (2020-02-27)
Non-clustered δ1- and δ2-protocadherins, close relatives of clustered protocadherins, function in cell adhesion and motility and play essential roles in neural patterning. To understand the molecular interactions underlying these functions, we used solution biophysics to characterize binding of δ1- and
Drew S Tack et al.
Molecular systems biology, 17(3), e10179-e10179 (2021-03-31)
Allostery is a fundamental biophysical mechanism that underlies cellular sensing, signaling, and metabolism. Yet a quantitative understanding of allosteric genotype-phenotype relationships remains elusive. Here, we report the large-scale measurement of the genotype-phenotype landscape for an allosteric protein: the lac repressor
Biochemical applications of poly (ethylene glycol)
Topchieva IN
Russ. Chem. Rev., 49(3), 260-260 (1980)
Chen Zhang et al.
Scientific reports, 7, 44708-44708 (2017-03-21)
Mitochondria are intracellular organelles involved in cell survival and death, and dysfunctions of mitochondria are related to neurodegenerative diseases. As the most abundant protein in the mitochondrial inner membrane, adenine nucleotide translocator 1 (ANT1) plays a critical role in mitochondrial
Cosimo Posth et al.
Cell, 175(5), 1185-1197 (2018-11-13)
We report genome-wide ancient DNA from 49 individuals forming four parallel time transects in Belize, Brazil, the Central Andes, and the Southern Cone, each dating to at least ∼9,000 years ago. The common ancestral population radiated rapidly from just one
Yin Zhang et al.
Theranostics, 10(23), 10823-10837 (2020-09-16)
Rationale: The forkhead box A1 (FOXA1) is a crucial transcription factor in initiation and development of breast, lung and prostate cancer. Previous studies about the FOXA1 transcriptional network were mainly focused on protein-coding genes. Its regulatory network of long non-coding
D D Smyth et al.
Cardiovascular drugs and therapy, 4(1), 297-300 (1990-02-01)
Previous studies have demonstrated that Separan AP-30, a drag-reducing polymer, significantly decreased the formation of atherosclerotic plaques in rabbits fed a high-cholesterol diet. Furthermore, Separan AP-273, a polymer similar to but longer than Separan AP-30, markedly increased cardiac output in
A simple, inexpensive, robust and sensitive dot-blot assay for equal detection of the nonstructural-1 glycoprotein of all dengue virus serotypes
Falconar AK and Romero-Vivas CM
Virology Journal, 10(1), 126-126 (2013)
Andrew K I Falconar et al.
Virology journal, 10, 126-126 (2013-04-24)
Detection of dengue virus (DENV) soluble/excreted (s/e) form of the nonstructural-1 (NS1) glycoprotein in patient acute-phase sera is ideal for diagnosis. The commercially-available detection assays are, however, too expensive for routine use and have low specificity, particularly for the s/e
Mark A Rice et al.
Acta biomaterialia, 5(1), 152-161 (2008-09-17)
Ultrasound has potential as a non-destructive analytical technique to provide real-time online assessments of matrix evolution in cell-hydrogel constructs used in tissue engineering. In these studies, chondrocytes were encapsulated in poly(ethylene glycol) hydrogels, and gel degradation was manipulated to provide
Jin Liu et al.
STAR protocols, 2(2), 100531-100531 (2021-05-25)
Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence
The effect of polyethylene glycol (PEG 8000) supplementation on in vitro gas production kinetics of leaves from tannin containing trees
Kamalak A, et al.
South African Journal of Animal Science, 35(4), 229-237 (2005)
M Patel Geeta et al.
Current drug delivery, 6(2), 159-165 (2009-05-20)
Carbamazepine indicated for the control of epilepsy, undergoes extensive hepatic first-pass metabolism after oral administration. A vaginal dosage form of carbamazepine is not commercially available. Conventional suppository having poor retention in the vaginal tract, as they are removed in a
Itze C Navarro-Hernandez et al.
The FEBS journal, 287(16), 3449-3471 (2020-01-21)
B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody-secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, regulated by the formation of different lipid-bilayer
Dmitry Lim et al.
International journal of molecular sciences, 19(10) (2018-10-03)
Astrocytes respond to neuronal activity by generating calcium signals which are implicated in the regulation of astroglial housekeeping functions and/or in modulation of synaptic transmission. We hypothesized that activity-induced calcium signals in astrocytes may activate calcineurin (CaN), a calcium/calmodulin-regulated protein
Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions
Zhang C, et al.
Scientific Reports, 7(1), 44708-44708 (2017)
See-Yeun Ting et al.
Cell host & microbe, 28(2), 313-321 (2020-05-30)
Selective and targeted removal of individual species or strains of bacteria from complex communities can be desirable over traditional, broadly acting antibacterials in several contexts. However, generalizable strategies that accomplish this with high specificity have been slow to emerge. Here
Athanasia Mizi et al.
Methods (San Diego, Calif.), 170, 33-37 (2019-07-10)
Genome organization is now understood to be tightly linked to all genomic functions. Thus, the high-resolution mapping of higher-order chromosomal structures via 3C-based approaches has become an integral tool for studying transcriptional and cell cycle regulation, signaling effects or disease
Characterisation of gliclazide-PEG 8000 solid dispersions
Biswal S, et al.
Tropical Journal of Pharmaceutical Research, 8(5) (2009)
Daniel J Wiegand et al.
Journal of visualized experiments : JoVE, (145) (2019-04-02)
The marine bacterium Vibrio natriegens has garnered considerable attention as an emerging microbial host for biotechnology due to its fast growth rate. A general protocol is described for the preparation of V. natriegens crude cell extracts using common laboratory equipment.
Polyethylene glycol (PEG): a versatile polymer for pharmaceutical applications
Dsouza AA and Shegokar R
Expert Opinion on Drug Delivery, 13(9), 1257-1275 (2016)
Idalis Villanueva et al.
Acta biomaterialia, 5(8), 2832-2846 (2009-06-11)
The pericellular matrix (PCM) surrounding chondrocytes is thought to play an important role in transmitting biochemical and biomechanical signals to the cells, which regulates many cellular functions including tissue homeostasis. To better understand chondrocytes interactions with their PCM, three-dimensional poly(ethylene
Xilin Wu et al.
The Journal of clinical investigation, 128(6), 2239-2251 (2018-02-21)
The discovery of an HIV-1 cure remains a medical challenge because the virus rebounds quickly after the cessation of combination antiretroviral therapy (cART). Here, we investigate the potential of an engineered tandem bispecific broadly neutralizing antibody (bs-bnAb) as an innovative
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