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Trizma HCl Specification Chart
ProductDetail?ProdNo=93363&Brand=SIGMA http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=T3253&Brand=SIGMA http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=93363&Brand=SIGMA
Brochure-Rethink Western Blotting
SDS-PAGE separating gel casting buffer [Tris-HCl]: 1.5 M For 500 mL: 118.2 g Tris-HCl (Product 93363 - FW:157.60 g/mol) Add 450 mL Milli-Q® water and adjust pH to 8.8 Add Milli-Q® water to final volume