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Trizma HCl Specification Chart
ProductDetail?ProdNo=93363&Brand=SIGMA
http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=T3253&Brand=SIGMA
http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=93363&Brand=SIGMA
Brochure-Rethink Western Blotting
SDS-PAGE separating gel casting buffer
[Tris-HCl]: 1.5 M
For 500 mL:
118.2 g Tris-HCl (Product 93363 - FW:157.60 g/mol)
Add 450 mL Milli-Q® water and adjust pH to 8.8
Add Milli-Q® water to final volume