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Amicon® Ultra-0.5 Centrifugal Filter Devices Non IVD User guide
number)
137 10
20
48
≥ 95
≥ 90
≥ 90
≥ 90
≥ 85
≥ 75
0
1
2
301 10
20
48
≥ 90
≥ 85
≥ 90
≥ 90
≥
User Guide -Amicon® Ultra-0.5 Centrifugal Filter Devices
number)
137 10
20
48
≥ 95
≥ 90
≥ 90
≥ 90
≥ 85
≥ 75
0
1
2
301 10
20
48
≥ 90
≥ 85
≥ 90
≥ 90
≥
Amicon® Ultra-0.5 Centrifugal Filter Devices for volumes up to 500 μL
number)
137 10
20
48
≥ 95
≥ 90
≥ 90
≥ 90
≥ 85
≥ 75
0
1
2
301 10
20
48
≥ 90
≥ 85
≥ 90
≥ 90
≥
Data Sheet - SRP5174
70–95% (densitometry)
26
56
130
170
43 CDC42
34
72
95
References
1. Erickson, J.W. et al., Multiple roles for Cdc42 in cell
regulation. Curr. Opin. Cell Biol., 13(2), 153-7
(2001
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
105 100 97 95 74 92 106 73 106 77 91 109
1:8 dil 123 84 48 108 98 91 94 63 87 105 70 110 66 84 111
1:16 dil 129 84 42 108 87 90 94
Data Sheet - 1.46138.0020
at 95 °C in a water bath (200 ml
bottles 30 minutes) and then cooled to 44-48 °C in a second water bath (time as above). The complete
process of liquefaction, cooling and tempering at 44-48
Data Sheet - 1.46370.0020
at 95 °C in a water bath (200-ml
bottles 30 minutes) and then cooled to 44-48 °C in a second water bath (time as above). The complete
process of liquefaction, cooling and tempering at 44-48 °C
Measuring Immune Response and Cytokine Autoantibodies in Human Sepsis Samples
10,000 0.27 4.02 4.82 95
IL-3 1.28-20,000 0.29 3.26 5.62 100
IL-4 0.64-10,000 0.20 4.55 5.94 95
IL-5 0.64-10,000 0.17 3.99 5.42 99
IL-6 0.64-10,000 0.14 5.06 6.13 96
IL-7 0.64-10,000 0.14 4.85
Product Information Sheet - SHH0027
minutes in Step 4.
b. Treat for 20–25 minutes for samples
stained for 48 hours in Step 4.
9. Dehydrate in the following:
a. 95% alcohol for 15 minutes.
b. 100% alcohol for 15 minutes.
User Guide-HCYTA60K
M-CSF 97
IL-1RA 97 MDC 96
IL-2 95 MIG 95
IL-3 100 MIP-1α 104
IL-4 95 MIP-1β 99
IL-5 99 PDGF-AA 102
IL-6
Product Information Sheet - AXIO0033
appropriate volume of medium (e.g., 1 mL per
24 well; 0.5 mL per 48 well).
4. Incubate the cells overnight at 37 °C with 7% CO2
and 95% humidity.
5. Next day, microscopically examine the endothelial
Product Information Sheet - AXIO0052
appropriate volume of medium (e.g., 1 mL per
24 well; 0.5 mL per 48 well).
4. Incubate the cells overnight at 37 °C with 7% CO2
and 95% humidity.
5. Next day, microscopically examine the endothelial
Development of a MILLIPLEX®MAP multiplexed assay panel for emerging biomarkers
-bleed 24 hrt 48 hr 72 hr 96 hr
MIP-1α EGF IL-5 MCP-1
GRO/KC/CINC-1 VEGF-A Fractalkine
3000
2500
2000
1000
1500
800
200
400
600
0
pre-bleed
Protocol- HCYTA-60K|HCYTA-60K-PX38|HCYTA-60K-PXBK38|HCYTA-60K-PX48|HCYTA-60K-PXBK48|HCYTPA-76K|HCYTPAB-76SK|HCYTPA-96K|HCYTPAB-96SK
M-CSF 97
IL-1RA 97 MDC 96
IL-2 95 MIG 95
IL-3 100 MIP-1α 104
IL-4 95 MIP-1β 99
IL-5 99 PDGF-AA 102
IL-6
User Guide
M-CSF 97
IL-1RA 97 MDC 96
IL-2 95 MIG 95
IL-3 100 MIP-1α 104
IL-4 95 MIP-1β 99
IL-5 99 PDGF-AA 102
IL-6
Eppendorf Mastercyclers Save Time and Cost in PCR
denaturation 95°C 10 min 95°C 10 min
2
(30 cycles)
Denaturation 95°C 30 s 95°C 30 s
Annealing aGradient °C 30 s
bGradient°C 60 s
Extension 72°C 60 s
3 Final Extension 72°C 7 min 72°C 7
Multiplex bead array approach for quantitative profiling of 95 cancer-immunity biomarkers in breast cancer
Cytokine/Chemokine/Growth Factor Panel
A (48-plex). This figure shows the Panel A multiplex standard curves
for the simultaneous quantification of these 48 analytes.
Figure 1. Human Immuno-Oncology
Data Sheet 1.46730.0020 Differential Reinforced Clostridial Medium (DRCM) Agar
perfringens
13124 100-1000
44-48 h at 35-37 °C
(anaerobic)
good growth; grey-whitish,
large, arched colonies
Clostridium
sordellii
9714 100-1000
44-48 h at 35-37 °C
(anaerobic)
Data Sheet - SRP5008
Typical Lot
70–95% (densitometry)
Figure 2.
Specific Activity of Typical Lot
32-48 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7. 2, 12.5 mM
glycerol
The Effective Use of Protein Kinase Inhibitors
97 53 74 23
96 102 60 71 50 30 22 1 95 59 6 72 36 93 84 94 14 26 90 101 82 61
48 75 38 99 86 8 6 54 66 10 83 105
Spare parts for Mobius®Bioreactors (50 L, 200 L, 1000 L & 2000 L)
1
5
76
50
97
96 87
94
95
76
2
84
78
79
51
3
80
82
4
8
User Guide-MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel B Magnetic Bead Panel
offer you:
• The ability to select a 38-plex or 48-plex premixed kit.
• The ability to choose any combination of analytes from our panel of
48 analytes to design a custom kit that better
User Guide-HCYTB60K
offer you:
• The ability to select a 38-plex or 48-plex premixed kit.
• The ability to choose any combination of analytes from our panel of
48 analytes to design a custom kit that better
LABORATORY NOTEBOOK ISSUANCE PAGE
39
.9
48
69
.7
23
72
.6
3
74
.9
22
78
.9
6
79
.9
04
83
.7
98
11
4.
82
11
8.
71
12
1.
76
12
7.
60
12
6.
90
13
1.
29
LABORATORY NOTEBOOK ISSUANCE PAGE
39
.9
48
69
.7
23
72
.6
3
74
.9
22
78
.9
6
79
.9
04
83
.7
98
11
4.
82
11
8.
71
12
1.
76
12
7.
60
12
6.
90
13
1.
29
Hybridoma and CHO Cell Culture with Eco-Friendly New Brunswick™ S41i Incubator Shaker
and competitor 2. The
flasks were incubated at 37°C in a mixture of 5% CO2 and
95% air. The units were agitated at 95 RPM (2.52 rcf).
Hybridoma cells were subcultured on day 2 and 4 to a
concentration
MMP Activation Chart
active-MMP-7 does not activate
APMA, 1mM 37oC, 1h A
TrypsinTPCK, 10µg/ml 37oC, 1-2h B
Imai et al., JBC270/'95/6691
Crabbe, et.al. Biochem.
31,1992,8500 MMP-7
Matrilysin
EX-CELL 420 Serum Medium for the Growth of Spodopteran (Sf9 and Sf21) Insect Cells
greater than 95% and
can be maintained for more than 10 days at these densities.
Cultures seeded at low density (2.5 x 105 cells/mL) over multiple
passages do not exhibit the typical 24 - 48 hour lag phase
LABORATORY NOTEBOOK ISSUANCE PAGE
39
.9
48
69
.7
23
72
.6
3
74
.9
22
78
.9
6
79
.9
04
83
.7
98
11
4.
82
11
8.
71
12
1.
76
12
7.
60
12
6.
90
13
1.
29
Data Sheet - MSST0014
Toxicology, 48, 463-493 (2008).
2. Wolf, M.W., and Boldt, J., Kidney specific proteins:
markers for detection of renal dysfunction after
cardiac surgery? Clin. Res. Cardiol. Suppl., 2,
S103–7 (2007).
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