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Raw Materials for Biopharmaceutical Manufacturing
Expert
110164 Emprove® Expert 1 L (PB), 2.5 L (PB), 25 L (PC), 200 L (PBC), 950
L (PIBC)
Hydrochloric Acid Fuming 37% Emprove®
Expert
137007 Emprove® Expert 1 L (GB), 2.5 L (GB), 25 L
Product Information Sheet - A9400
D
Product Number A 9400
Storage Temperature 2-8 °C
Product Description
Molecular Formula: C62H87N13O16
Molecular Weight: 1,270
CAS Number: 7240-37-1
Synonym: 7-AAD
Fluorescent Properties
Free form
Extractables test report: Solutions in polyethylene containers
3 and 13 months storage at 40 °C and at room temperature.
6
HCl 37%, 200 L PE drum HCl 25%, 950 L IBC
Element Start Value
Product Information Sheet - CYTOCOX1
Ferrocytochrome c
Substrate Solution
(l)
Blank 950 100 – 50
Unknown
sample
950 (100–x) x 50
Positive
control
950 60-80 20-40 50
1. Add 0.95
User Guide-LSKMAGD12
, continue to mix while pipetting.
http://www.SigmaAldrich.com
2 of 4
3. Pipette 950 μL of the resuspended beads into a
2 mL microcentrifuge tube. Place the tube into
the
Data Sheet - 00185 (Twin Pack)
precipitate.
Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check
the pH and if necessary, adjust. Make up to 1 litre and pour into tubes.
Data Sheet - 00185 (Powder)
Colourless, clear to slightly turbid solution with some precipitate.
Directions:
Dissolve 2.56 g in 950 ml distilled water. Check the pH and if necessary adjust. Make up to 1 litre and pour into tubes.
Data Sheet - 11012
0.2 at 37 °C
Store prepared media below 8 °C, protected from direct light. Store dehydrated powder in a dry place in tightly-sealed
containers at 2-25 °C.
Directions:
Dissolve 52.6 g in 950 ml distilled
Quality Control Range Sheet- A6602-107 and A6602-207
IP-10 Control 1 90 – 186 pg/mL TNF α Control 1 241 – 501 pg/mL
Control 2 458 – 950 pg/mL Control 2 1107 – 2299 pg/mL
Data Sheet - M8065
Dispense 8 µl of 1.25X Reaction Mix into each tube.
4. Start each reaction by the addition of 2 µl of the
appropriate diluted enzyme sample at 20 second
intervals.
5. Incubate for 10 minutes at 37 °C.
Product Information Sheet - SRE0066
Bruice, P.Y., Organic Chemistry, 3rd Ed., Prentice Hall
(Upper Saddle River, New Jersey: 2001), pp 950-953.
2. Cox, R.A., The Use of Guanidinium Chloride in the
Isolation of Nucleic Acids, Methods
Data Sheet - 91019
coloured clear to slightly opalescent gel forms in Petri plates
Directions:
Suspend 43.41 grams in 950 ml distilled water. Heat to boiling with frequent agitation to dissolve the
medium completely. Sterilize
Data Sheet - 43314
Slightly yellow coloured, opalescent gel.
Directions:
Suspend 62.02 grams of dehydrated media in 950 ml distilled water (1 liter for non-selective media). Heat to boiling to
dissolvethe medium completely
LIGHT DIAGNOSTICS™ HSV 1 & 2 Typing DFA Kit - (Spanish, Italian, German & French Translation)
someta la muestra a
ultrasonido de 8 a 12 kc/seg. durante no más de un minuto. Deseche la torunda
en una solución de hipoclorito de sodio. Centrifugue la muestra a 950 x g
durante 7-10 minutos, deseche
Product Information Sheet - SRE0066
Bruice, P.Y., Organic Chemistry, 3rd Ed.,
Prentice Hall (Upper Saddle River, New Jersey:
2001), pp 950-953.
2. Cox, R.A., The Use of Guanidinium Chloride in
the Isolation of Nucleic Acids, Methods
Data Sheet - G7294
(For more
information, see the Technical Bulletin for Product
Code T 6567.)
8. Incubate the sample overnight at 37 °C in a water
bath.
9. Peptide solutions can now be analyzed by mass
spectrometric
Data Sheet - P6782
Information
Peroxidase from horseradish
Sigma Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using
ABTS), ≥250 units/mg solid (using pyrogallol)
P6782
Product
Nick Translation DNA Labeling Systems
Bio-16-dUTP labeled DNA
after 1 hour
after 2 hours
after 5 hours
175 ng
300 ng
500 ng
590 ng
950 ng
1340 ng
1550 ng
DNA synthesis was measured by incorporation of 3H-dNTP
PURIFICATION OF
Data Sheet - SRP5294
tissues, with the highest
expression in heart and skeletal muscle.
Recombinant human MEKK6 (620-950) was expressed
by baculovirus in Sf9 insect cells using an N-terminal
GST-tag. The MEKK6 gene accession
Product Information Sheet - MAK349
water to
generate a 1 mM solution.
2. Further dilute the 1 mM solution by adding 50 L to
950 L of ultrapure water, yielding a 50 M
Polyamine Standard working solution.
Product Information Sheet - 11828673001
before use.
– DMSO-containing solutions may solidify upon storage at +2 to +8°C. If this occurs, incubate Bottle 1 at +37°C
in a water bath and vortex until solution appears clear.
Dilute 1 part
Product Information Sheet - GUSA
determined according to
calibration procedure B. Keep the tubes on ice.
7. Cap tubes and incubate at 37°C.
8. Transfer 100 :l aliquots at 10 minute intervals
to vials containing 2 ml cold stop solution.
Product Information Sheet - MAK097
standard to 0.5 mM by diluting 50 l of the
10 mM standard solution with 950 l of GOx Assay
Buffer. Add 0, 2, 4, 6, 8, 10 L of the 0.5 mM standard
solution into
Product Information Sheet - MAK353
1. Prepare a 1.235 M Methanol Solution by adding
50 L of the 24.7 M Pure Methanol Stock to 950 L
of ultrapure water and mix.
2. Dilute the 1.235 M Methanol Solution to 50 mM
Cell Culture Media 4CHO Sample Kit
screening sample 1 15 30 60
No. of trial samples from 1 L basal medium* 950 63 31 15
Note*: Calculations based on a final volume of 950 mL liquid medium to consider potential losses during medium
preparation
Product Information Sheet - N9155
precipitation step once).
References
1. Klett, R.P. et al., Proc. Natl Acad Sci USA, 60,
946-950 (1968)
2. Rigby, P.W.J., et al., J. Mol. Biol., 113, 237-251
(1977)
Product Information Sheet - N8530
precipitation step once).
References
1. Klett, R.P. et al., Proc. Natl Acad Sci USA, 60,
946-950 (1968)
2. Rigby, P.W.J., et al., J. Mol. Biol., 113, 237-251
(1977)
Product Information Sheet - MAK117
Standards
Number
Glycerol
Standard
(1 mM)
Water
Final Glycerol
Concentration
1 50 L 950 L 0.050 mM
2 30 L 970 L 0.030 mM
3 15 L 985 L
MAK416 - Technical Bulletin
1. Prepare a 100 M Standard solution by
diluting 50 L of the 2 mM Fluorescence
Standard with 950 L of purified water.
2. Prepare a 5 M Standard solution by
adding 10 L of the 100 M
Product Information Sheet - MAK020
Take 50 µL of the 1 mM
standard solution and add to 950 µL of water to make a
0.05 mM Fructose-6-Phosphate standard solution. Add
0, 2, 4, 6, 8, and 10 µl of the 0.05 mM Fructose-6-
Phosphate standard
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