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Product Information Sheet-SRP0730
from Lipid Kinase
Buffer 5x; L01-09).
• Substrate (P426-59)
PI(3)P:PS substrate solution contains 250 µM
of PI and 2000 µM of PS in Lipid Dilution Buffer
(L21-09).
Assay Protocol
The PIP5K3
Product Information Sheet - CASPASSYRO
y Version 09
Content version: February 2019
Cellular Assay for the quantitative in vitro determination of caspases activity in
microplates
Cat. No. 03 005 372 001 100 tests on 96-well plates
Protocol- 48-614MAG
procedures.
48-614MAG Rev 09/22 11
Cell lysis protocol for cells in sterile 96-well tissue culture plates
Adherent or non-adherent cells seeded or grown in sterile 96-well tissue culture grade
Protocol- 48-618MAG
MILLIPLEX® 96-well Plates with sealers MAG-PLATE
For research use only. Not for use in diagnostic procedures.
48-618MAG Rev
Product Information Sheet - MAK514
samples)
mak514pis Rev 09/23
3
User Guide - EC196
Manual
GenElute™-E Single Spin
Blood DNA 96 Kit
For 96-Well Purification of Genomic DNA from Liquid Blood Samples
EC196
20448859w Rev 09/20 2 of 10
Introduction
GenElute™-E Single
Product Information Sheet - MAK531
Rev 09/23
3
Results
1. Subtract the blank value (standard #4) from all
the standard values.
2. Plot the ΔOD
Protocol- 48-624MAG
has sufficient reagents for one 96-well
plate assay.
For research use only. Not for use in diagnostic procedures.
48-624MAG
Protocol- 48-621MAG
use in diagnostic procedures.
48-621MAG Rev 09/22 1
User Guide
MILLIPLEX® 7-Plex DNA
Damage/Genotoxicity Magnetic Bead Kit
96-Well Plate
Protocol- 48-622MAG
procedures.
48-622MAG Rev 09/22 10
Suggested cell lysis protocol for cells in sterile 96-well tissue culture plates
Adherent or non-adherent cells grown in sterile 96-well tissue culture grade
Protocol- 48-611MAG
use in diagnostic procedures.
48-611MAG Rev 09/22 1
User Guide
MILLIPLEX® Akt/mTOR Phosphoprotein
11-plex Magnetic Bead Kit
96-Well Plate
User Guide - EC396
User Manual
GenElute™-E Single Spin
Tissue DNA 96 Kit
For 96-Well Purification of Genomic DNA from Tissue Samples
EC396
20448855w Rev 09/20 2 of 10
Introduction
GenElute™-E Single Spin
(G5003) - Enzyme Assay
milliliters) of reaction mix used in the colorimetric determination
SSNITR01 Page 3 of 3
Revised: 08/09/96
CALCULATIONS:
units/ml enzyme
Units/mg solid
(G0660) - Enzyme Assay
milliliters) of reaction mix used in the colorimetric determination
SSNITR01 Page 3 of 3
Revised: 08/09/96
CALCULATIONS:
units/ml enzyme
Units/mg solid
Enzymatic Assay of ESTERASE
mM borate, 0.2% (v/v) ethyl butyrate
and 5 units esterase.
PEETHY02 Page 3 of 3
Revised: 09/01/96
Enzymatic Assay of ESTERASE
(EC 3.1.1.1)
Sigma
Enzymatic Assay of a-GLUCOSIDASE
milliliters) of reaction mix used in the colorimetric determination
SSNITR01 Page 3 of 3
Revised: 08/09/96
CALCULATIONS:
units/ml enzyme
Units/mg solid
Product Information Sheet - MAK494
µL 90 µL 50
2 6 µL 94 µL 30
3 3 µL 97 µL 15
4 0 µL 100 µL 0
3. Mix well and transfer 10
Product Information - P6306
unit is equivalent to approximately 0.004 casein digestion unit.
SPGLUT04 Page 3 of 3
Revised: 09/23/96
Enzymatic Assay of PROTEASE
(EC 3.4.21.19)
Sigma Prod Nos. P-2922, P-6306, and P-8400
(G3651) - Enzyme Assay
milliliters) of reaction mix used in the colorimetric determination
SSNITR01 Page 3 of 3
Revised: 08/09/96
CALCULATIONS:
units/ml enzyme
Units/mg solid
(E3019) - Enzyme Assay
mM borate, 0.2% (v/v) ethyl butyrate
and 5 units esterase.
PEETHY02 Page 3 of 3
Revised: 09/01/96
Enzymatic Assay of ESTERASE
(EC 3.1.1.1)
Sigma
(E2884) - Enzyme Assay
mM borate, 0.2% (v/v) ethyl butyrate
and 5 units esterase.
PEETHY02 Page 3 of 3
Revised: 09/01/96
Enzymatic Assay of ESTERASE
(EC 3.1.1.1)
Sigma
Product Information Sheet - MAK483
KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
mak483pis Rev 09/22
3
Product Information Sheet - MAK487
each Standard into
separate wells of a black 96-well plate.
mak487pis Rev 09/22
Product Information Sheet - MAK534
65 µL + 35 µL 13
3 30 µL + 70 µL 6
4 0 µL + 100 µL 0
Measurement
96-well plate:
1. Transfer 20 µL of standards into
Product Information Sheet - MAK502
mak502pis Rev 09/23 3
Results
1. Subtract the water blank (Std #8) value from all
the Standard values.
2. Plot the ΔRFU or ΔOD against the
Product Information Sheet - MAK533
50 = reaction volumes (µL)
40 = sample volumes (µL)
mak533pis Rev 09/23
3
Product Information Sheet - H7006
H7006 Page 3 of 3
09/17/96 - CKV
HEPES SODIUM SALT
Sigma Prod. No. H7006
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon, E.L.
Product Information Sheet - MAK539
mak539pis Rev 09/23
2
Procedure
All Samples and Standards should be run in duplicate.
Note:
This procedure is written for 96-well
Product Information Sheet - MAK008
well, and incubate for
90 minutes at 60 °C.
3. Measure the absorbance at 560 nm (A560).
mak008 Rev 09/23
Product Information Sheet - H9136
H3375 Page 3 of 3
09/17/96 - CKV
HEPES FREE ACID
Sigma Prod. No. H3375, H7523, H6147, H9136 and H4034
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon
Page 1 of 23