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Automated Protocol for Extract-N-Amp Tissue PCR Kits Using the Biomek FX (Beckman Coulter)
setup for
96 tissue samples.
Page 3 of 17
II. Product Components
III. Storage
Product Information Sheet - XNAT2R
setup for
96 tissue samples.
Page 3 of 17
II. Product Components
III. Storage
Automated Protocol for Extract-N-Amp Tissue PCR Kits
setup for
96 tissue samples.
Page 3 of 17
II. Product Components
III. Storage
Magna ChIP™ HT96
plate (1)
Plate seals (3)
Strip caps (12)
Magna ChIP™ HT96 Thermal Plate Set 17-10458
96-well Thermal Plate (1)
Strip caps (12)
Magna ChIP™ HT96 ChIP Plate Set 17-10459
96-well ChIP plates (2)
EZ-Magna ChIP™ HT96 (Cat. No. 17-10078)
plate (1)
Plate seals (3)
Strip caps (12)
Magna ChIP™ HT96 Thermal Plate Set 17-10458
96-well Thermal Plate (1)
Strip caps (12)
Magna ChIP™ HT96 ChIP Plate Set 17-10459
96-well ChIP plates (2)
Magna ChIP™ HT96 (Cat. No. 17-10077)
plate (1)
Plate seals (3)
Strip caps (12)
Magna ChIP™ HT96 Thermal Plate Set 17-10458
96-well Thermal Plate (1)
Strip caps (12)
Magna ChIP™ HT96 ChIP Plate Set 17-10459
96-well ChIP plates (2)
User Guide 1.06887
3 min
Ethanol 96 % 30 sec
Ethanol 96 % 30 sec
Papanicolaou’s solution 3a polychromatic solution EA 31
or
Papanicolaou’s solution 3b polychromatic solution EA 50
3 min
Ethanol 96 % 30
User Guide (IFU) - 1.06888
3 min
Ethanol 96 % 30 sec
Ethanol 96 % 30 sec
Papanicolaou’s solution 3a polychromatic solution EA 31
or
Papanicolaou’s solution 3b polychromatic solution EA 50
3 min
Ethanol 96 % 30
Magna ChIP™ HiSens Kits
isolation
17-10078
Magna ChIP™ HT96 ChIP and
Thermal Plate Set
Magna ChIP™ HT96 kit accessory to allow running of partial plates. Inlcudes 96-well Thermal
Plate and set of Strip Caps, 96-well ChIP
STAR Phospho-p53 (Ser15) ELISA Kit
Microsoft Word - 17-475-manual.doc
Instruction Manual
For
STAR Phospho‐p53 (Ser15) ELISA Kit
Catalog # 17‐475
Sufficient reagents for 96 assays per kit
Contents
Product Information Sheet - CASP8C
60 minutes or even longer for
very dilute samples).
3
Table 1.
Reaction Scheme for 96 Well Plate Microassay Method
EdU Cell Proliferation Assay
of 3% BSA in PBS.
5. Proceed to Fluorescent EdU Detection steps.
6
Flow Cytometry
I. EdU Incorporation Procedure
1. Plate cells
Spare parts for Mobius®Bioreactors (50 L, 200 L, 1000 L & 2000 L)
One among 17
to 38 depending
on bioreactor
configuration
(cap included
—not shown)
(nipple only)
86
1
91
97
82
57
83
96
87
80
52
51
FlowCellect™ Mouse Th17 Differentiation Tool Kit
plate.
17. Incubate at 37ºC in a CO2 humidified incubator for 3-5 hours.
18. Harvest cells for analysis.
This step can be scaled up or down using 6- or 96-well plates as follows;
Product Information Sheet - CASP3C
For assays using 96-well plates, dilute
the 2 mM stock solution to 200 µM with
1× Assay Buffer.
• Caspase 3 positive control:
o Reconstitute the vial (5 µg) with 50 µL
H2A.X Phosphorylation Assay Kit Chemiluminescence Detection
Chemiluminescence Detection
Catalog #17-327
Chemiluminescent ELISA System for Detection of
DNA Double-Strand Breaks and/or Apoptosis.
Sufficient reagents for two 96-well plate assays per kit.
Product Information Sheet - H7006
H7006 Page 3 of 3
09/17/96 - CKV
HEPES SODIUM SALT
Sigma Prod. No. H7006
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon, E.L.
Product Information Sheet - H6147
H3375 Page 3 of 3
09/17/96 - CKV
HEPES FREE ACID
Sigma Prod. No. H3375, H7523, H6147, H9136 and H4034
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon
Product Information Sheet - H4034
H3375 Page 3 of 3
09/17/96 - CKV
HEPES FREE ACID
Sigma Prod. No. H3375, H7523, H6147, H9136 and H4034
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon
Product Information Sheet - H9136
H3375 Page 3 of 3
09/17/96 - CKV
HEPES FREE ACID
Sigma Prod. No. H3375, H7523, H6147, H9136 and H4034
CITED REFERENCES:
1. Sigma quality control.
2. Merck Index, 12th Ed., #4687 (1996).
3. Medzon
GSK-3β STAR ELISA Kit
3
V. KIT COMPONENTS
1. GSK-3β Capture Plate: (Part No. 17-471A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
2. Anti-GSK-3β detection antibody: (Part No. 17-471B) One
phospho-IRS1 (Ser312) STAR ELISA Kit
anti-IRS1 antibody: (Part No. 17-459A) One pre-
coated 96-stripwell immunoplate sealed in a foil pouch.
2. Anti-phospho-IRS1 (Ser312) detection antibody: (Part No. 17-459B) One bottle
(11 mL) of anti-phospho-IRS1
Product Information Sheet - M9069
cocktail,
PerkinElmer Product Code 1200-439
• Ultrapure water (17 MΩ⋅cm or equivalent)
• Filter plates (Millipore MultiScreenHTS 96 well Filter
Plates with glass fiber filter or equivalent, Millipore
MEK1 STAR ELISA Kit
Microsoft Word - 17-473MAN.doc
Instruction Manual For
MEK1 STAR ELISA Kit
Catalog # 17-473
Sufficient reagents for 96 assays
IêB STAR ELISA Kit
Microsoft Word - 17-485MAN.doc
Instruction Manual For
IκB STAR ELISA Kit
Catalog # 17-485
Sufficient reagents
STAR AKT1 ELISA Kit
Microsoft Word - 17-455-Manual.doc
Instruction Manual
For
STAR AKT1 ELISA Kit
Catalog # 17‐ 455
Sufficient reagents for 96 assays per kit
Contents Page
I. TEST
Protocol: GLP-1 Total ELISA
-5439
emdmillipore.com
EZGLP1T-36K Rev. 8-May-17 EMD Millipore
STAR phospho-MEK1 (Ser218/222) ELISA Kit
Microsoft Word - 17-474.doc
Instruction Manual For
Phospho‐MEK1 (Ser218/222)
STAR ELISA Kit
Catalog # 17‐ 474
Sufficient reagents for 96 assays per kit
Contents
Product Information Sheet - CASP3F
Note: For assays using 96 well plates dilute the stock
solution to 200 µM with 1× Assay Buffer.
Caspase 3 Positive Control - Reconstitute the vial
(5 µg) with 50 µl of 17 megohm water (100 µg/ml)
Product Information Sheet - F4428
D8537)
• Caspase-3 assay kit (CASP-3-F)
• 17 megohm water
• 50 ml disposable tube
• Four 15 ml disposable tube
• Two PCR tube strips - 0.2 ml
• 96 well plate, flat bottom
• 96
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