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Showing 1-30 of 31 results for "A2024" within Papers
Paulo Martins da Costa et al.
Veterinary microbiology, 139(3-4), 284-292 (2009-07-08)
The present study investigates, under field conditions, the influence of antimicrobial administration on prevalence and patterns of antimicrobial resistance among Escherichia coli and Enterococcus spp. isolated from growing broilers. For this purpose, a group of 16,000 commercial broiler chickens was
W Hong et al.
Letters in applied microbiology, 55(1), 33-39 (2012-04-19)
To engineer Streptomyces tenebrarius for producing carbamoyl tobramycin as a main component. The aprH-M gene fragment (apramycin biosynthetic gene from GenBank) in S. tenebrarius Tt49 was knocked out by genetic engineering to form S. tenebrarius T106 (ΔaprH-M). Compared to the
Jiro Kondo et al.
Nucleic acids symposium series (2004), (49)(49), 253-254 (2006-12-08)
Aminoglycosides are highly effective antibacterial drugs that decrease translation accuracy by binding to the aminoacyl-tRNA decoding site (A site) of 16S ribosomal RNA. On the other hand, they are highly toxic to mammals through kidney and ear-associated illnesses by binding
C M Yates et al.
Biology letters, 2(3), 463-465 (2006-12-07)
The study of antibiotic resistance has in the past focused on organisms that are pathogenic to humans or animals. However, the development of resistance in commensal organisms is of concern because of possible transfer of resistance genes to zoonotic pathogens.
G J Gunn et al.
Veterinary journal (London, England : 1997), 175(3), 416-418 (2007-04-24)
A random survey of farms in the Highlands and Islands of Scotland provides estimated of the prevalence of calves, finishers and cows carrying ampicillin, apramycin and/or nalidixic acid resistant Escherichia coli. While the survey provides information on the geographical variation
Liying Zhang et al.
Journal of AOAC International, 90(4), 885-891 (2007-09-01)
A high-performance liquid chromatographic method for determining apramycin in animal feeds was developed. Apramycin in feeds was extracted with 0.1 M HCl solution and cleaned up with an MCX solid-phase extraction column. The purified extract was derivatized with o-phthaldehyde, and
Marta Fedoryshyn et al.
Gene, 419(1-2), 43-47 (2008-06-14)
We report here a system for the functional expression of the Flp recombinase in several actinomycetes: Streptomyces coelicolor, S. lividans, and Saccharotrix espanaensis. We have constructed a synthetic gene encoding the Flp recombinase with a GC content of 60.6% optimized
D Balenci et al.
Dalton transactions (Cambridge, England : 2003), (7)(7), 1123-1130 (2009-03-27)
The interaction of apramycin with copper at different pH values was investigated by potentiometric titrations and EPR, UV-vis and CD spectroscopic techniques. The Cu(II)-apramycin complex prevailing at pH 6.5 was further characterized by NMR spectroscopy. Metal-proton distances derived from paramagnetic
Xiu Ying Zhang et al.
Research in veterinary science, 87(3), 449-454 (2009-06-09)
The aminoglycoside apramycin has been used widely in animal production in China since 1999. This study was aimed to investigate the resistance pattern of apramycin-resistant Escherichia coli isolated from farm animals and farm workers in northeastern of China during 2004-2007
Amanda M Fenner et al.
Carbohydrate research, 346(17), 2792-2800 (2011-10-22)
Synthesis of amphiphilic oligosaccharides is problematic because traditional methods for separating and purifying oligosaccharides, including sulfated oligosaccharides, are generally not applicable to working with amphiphilic sugars. We report here RPIP-LC and LC-MS methods that enable the synthesis, separation, and characterization
Suk-Kyung Lim et al.
Microbial drug resistance (Larchmont, N.Y.), 17(4), 563-566 (2011-09-13)
To investigate apramycin resistance in humans in Korea, a total of 138 human Escherichia coli strains confirmed as gentamicin-resistant were collected from Korean Culture Collection Antimicrobial-Resistant Microbes. Apramycin resistance (minimum inhibitory concentrations ≥1,024 μg/ml) was observed in 16 (11.6%) of the
Maksym Myronovskyi et al.
Applied and environmental microbiology, 77(15), 5370-5383 (2011-06-21)
Here we describe a versatile and sensitive reporter system for actinomycetes that is based on gusA, which encodes the β-glucuronidase enzyme. A series of gusA-containing transcriptional and translational fusion vectors were constructed and utilized to study the regulatory cascade of
Thomas Hermann et al.
Blood cells, molecules & diseases, 38(3), 193-198 (2007-01-30)
Aminoglycoside antibiotics bind specifically to the bacterial ribosomal decoding-site RNA and thereby interfere with fidelity but not efficiency of translation. Apramycin stands out among aminoglycosides for its mechanism of action which is based on blocking translocation and its ability to
Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy.
Duccio Balenci et al.
Chembiochem : a European journal of chemical biology, 11(2), 166-169 (2009-12-22)
C M Yates et al.
The Journal of antimicrobial chemotherapy, 54(2), 534-537 (2004-07-03)
The aminoglycoside apramycin has been used extensively in animal husbandry in the UK since 1978. This study aimed to determine both whether calves that had never been treated with aminoglycoside antibiotics harboured apramycin-resistant (apr(R)) commensal Escherichia coli, and the mode
Min-Jung Choi et al.
Foodborne pathogens and disease, 8(1), 119-123 (2011-01-11)
A total of 1921 Escherichia coli isolated from healthy animals (501 from cattle, 832 from pigs, and 588 from chickens) and 237 isolates from diseased pigs were tested to determine the prevalence of apramycin and gentamicin resistance in Korea during
Andrea T Fessler et al.
Antimicrobial agents and chemotherapy, 55(1), 373-375 (2010-09-30)
A novel apramycin resistance gene, apmA, was detected on the ca.-40-kb resistance plasmid pAFS11 from bovine methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398). The apmA gene coded for a protein of 274 amino acids that was related only
Kristina Kadlec et al.
The Journal of antimicrobial chemotherapy, 67(10), 2342-2345 (2012-06-22)
The aims of this study were to identify small staphylococcal plasmids that carry either the trimethoprim resistance gene dfrK or the apramycin resistance gene apmA and analyse them for their structure and organization with regard to their potential role as
T-W Hahn et al.
Journal of animal science, 84(6), 1422-1428 (2006-05-16)
Two experiments were conducted to evaluate the efficacy of beta-glucan on growth performance, nutrient digestibility, and immunity in weanling pigs. In Exp. 1, 210 weanling pigs (6.38 +/- 0.92 kg of BW) were fed dietary beta-glucan (0, 0.01, 0.02, 0.03
Yonghai Li et al.
The Journal of antibiotics, 61(6), 347-355 (2008-08-01)
Genetic manipulation of geldanamycin (GDM) producer Streptomyces species is a rational approach to understand biosynthesis processes and create new analogues. In this study, the carbamoyltransferase gene gdmN was inactivated by insertion of an apramycin-resistance gene aac3 (IV) into the genome
Vibeke F Jensen et al.
The Journal of antimicrobial chemotherapy, 58(1), 101-107 (2006-05-20)
Resistance towards the veterinary drug apramycin can be caused by the aac(3)-IV gene, which also confers resistance towards the important human antibiotic gentamicin. The objectives of this study were to investigate the temporal occurrence and the genetic background of apramycin
S Perzynski et al.
European journal of biochemistry, 99(3), 623-628 (1979-09-01)
1. The novel aminoglycoside antibiotic apramycin is shown to be a potent inhibitor of protein synthesis in bacteria both in vivo and in vitro. 2. In cell-free systems from Escherichia coli programmed with poly(U), apramycin induces translation errors, as assayed
Tanja Matt et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10984-10989 (2012-06-16)
Aminoglycosides are potent antibacterials, but therapy is compromised by substantial toxicity causing, in particular, irreversible hearing loss. Aminoglycoside ototoxicity occurs both in a sporadic dose-dependent and in a genetically predisposed fashion. We recently have developed a mechanistic concept that postulates
Jiro Kondo et al.
Chembiochem : a European journal of chemical biology, 8(14), 1700-1709 (2007-08-21)
The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used
Aalap Mogre et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 21(6), 711-726 (2014-10-05)
Evolution of bacteria under sublethal concentrations of antibiotics represents a trade-off between growth and resistance to the antibiotic. To understand this trade-off, we performed in vitro evolution of laboratory Escherichia coli under sublethal concentrations of the aminoglycoside kanamycin over short
Albert Tsai et al.
Cell reports, 3(2), 497-508 (2013-02-19)
Inferring antibiotic mechanisms on translation through static structures has been challenging, as biological systems are highly dynamic. Dynamic single-molecule methods are also limited to few simultaneously measurable parameters. We have circumvented these limitations with a multifaceted approach to investigate three
R Doug Wagner et al.
Antimicrobial agents and chemotherapy, 52(4), 1230-1237 (2008-01-30)
A bioassay was developed to measure the minimum concentration of an antimicrobial drug that disrupts the colonization resistance mediated by model human intestinal microbiota against Salmonella invasion of Caco-2 intestinal cells. The bioassay was used to measure the minimum disruptive
Mingxuan Xu et al.
Science in China. Series C, Life sciences, 49(4), 362-369 (2006-09-23)
Apramycin is unique in the aminoglycoside family due to its octodiose moiety. However, either the biosynthesis process or the precursors involved are largely unknown. Addition of glycine, as well as serine or threonine, to the Streptomyces tenebrabrius UD2 fermentation medium
Serena Bernacchi et al.
Nucleic acids research, 35(21), 7128-7139 (2007-10-19)
Owing to a striking, and most likely fortuitous, structural and sequence similarity with the bacterial 16 S ribosomal A site, the RNA kissing-loop complex formed by the HIV-1 genomic RNA dimerization initiation site (DIS) specifically binds 4,5-disubstituted 2-deoxystreptamine (2-DOS) aminoglycoside
Nasima Afsharimani et al.
Nanomaterials (Basel, Switzerland), 10(6) (2020-06-04)
In the present work, nanostructured graphene nanosheets were added to hybrid silica sols and deposited on aluminium alloy A2024-T3 to study the effect on the corrosion behaviour. Sols were prepared using tetraethyl-orthosilicate (TEOS), 3-glycidoxypropyl-trimethoxysilane (GPTMS) and a colloidal silica suspension
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