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Showing 1-30 of 205 results for "A2172" within Papers
Veronika Sander et al.
Nature protocols, 8(4), 800-809 (2013-03-30)
This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After collagenase digestion of the ventricle, cells are exposed to
Sue Ellen Verbrugge et al.
Oncotarget, 7(5), 5240-5257 (2015-10-27)
Aminopeptidase inhibitors are receiving attention as combination chemotherapeutic agents for the treatment of refractory acute myeloid leukemia. However, the factors determining therapeutic efficacy remain elusive. Here we identified the molecular basis of acquired resistance to CHR2863, an orally available hydrophobic
Panagiota A Sotiropoulou et al.
Methods in molecular biology (Clifton, N.J.), 407, 245-263 (2008-05-06)
Mesenchymal stem cells or marrow stromal cells (MSCs) represent a multipotent adult cellular population with immunomodulatory functions. In the adult human body, they are present in various niches, but their main source is bone marrow (BM). The regeneration capability of
Bingchuan Geng et al.
Redox biology, 52, 102295-102295 (2022-03-28)
Ubiquitin carboxyl-terminal esterase L1 (UCHL1) has been thought to be a neuron specific protein and shown to play critical roles in Parkinson's Disease and stroke via de-ubiquiting and stabilizing key pathological proteins, such as α-synuclein. In the present study, we
Gaoliang Yan et al.
International journal of molecular medicine, 36(4), 1001-1011 (2015-08-28)
The increased proliferation and migration of vascular smooth muscle cells (VSMCs) are key events in the development of artery restenosis following percutaneous coronary intervention. Digoxin has long been used in the treatment of heart failure and has been shown to inhibit
Dawn A Delfín et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 20(7), 1378-1383 (2012-05-02)
Identification of new molecular targets in heart failure could ultimately have a substantial positive impact on both the health and financial aspects of treating the large heart failure population. We originally identified reduced levels of the cell junction protein claudin-5
Said Abdalla et al.
Cardiovascular & hematological agents in medicinal chemistry, 9(3), 190-206 (2011-06-30)
Chronic pressure overload and atherosclerosis are primary etiologic factors for cardiac hypertrophy and failure. However, mechanisms underlying the transition from hypertrophy to heart failure are incompletely understood. We analyzed the development of heart failure in mice with chronic pressure overload
Chinedu U Nworu et al.
Methods (San Diego, Calif.), 66(3), 370-379 (2013-06-29)
Mutations in several sarcomeric proteins have been linked to various human myopathies. Therefore, having an in vivo developmental model available that develops quickly and efficiently is key for investigators to elucidate the critical steps, components and signaling pathways involved in
Laura E Briggs et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 245(2), 103-113 (2015-08-25)
The dorsal mesenchymal protrusion (DMP) is a second heart field (SHF) derived tissue involved in cardiac septation. Molecular mechanisms controlling SHF/DMP development include the Bone Morphogenetic Protein and Wnt/β-catenin signaling pathways. Reduced expression of components in these pathways leads to
T R Samelman
Experimental physiology, 85(1), 92-102 (2000-02-09)
Heat shock proteins (HSPs) are expressed when cells are exposed to various types of stress and they may provide protection against cellular insult. Previous data have shown increases in HSP expression following acute exhaustive exercise in rats (Locke et al.
Joshua J Martindale et al.
The Journal of biological chemistry, 280(1), 669-676 (2004-10-20)
The mitogen-activated protein kinases (MAPK) have been the subject of many studies to identify signaling pathways that promote cell survival or death. In cultured cardiac myocytes, p38 MAPK promotes cell survival or death depending on whether it is activated by
William Schubert et al.
The Journal of cell biology, 161(5), 861-874 (2003-06-11)
Nuclear factor of activated T cell (NFAT) is a ubiquitous regulator involved in multiple biological processes. Here, we demonstrate that NFAT is temporally required in the developing atrial myocardium between embryonic day 14 and P0 (birth). Inhibition of NFAT activity
Shih-Lin Chang et al.
Experimental and therapeutic medicine, 13(2), 771-779 (2017-03-30)
The impact of heart failure (HF) on sinoatrial node (SAN) channel regulation and electropharmacological responses has remained elusive. The present study aimed to investigate the effects of HF on the electrical activity of SANs with and without pharmacological interventions. Action
Lynn Yap et al.
NPJ Regenerative medicine, 8(1), 26-26 (2023-05-27)
Ischemic heart disease, which is often associated with irreversibly damaged heart muscle, is a major global health burden. Here, we report the potential of stem cell-derived committed cardiac progenitors (CCPs) have in regenerative cardiology. Human pluripotent embryonic stem cells were
Tim Snijders et al.
The Journal of nutrition, 144(2), 137-145 (2013-12-07)
Skeletal muscle satellite cells (SCs) play an important role in the myogenic adaptive response to exercise. It remains to be established whether nutrition plays a role in SC activation in response to exercise. In the present study, we assessed whether
Tara A Burns et al.
Anatomical record (Hoboken, N.J. : 2007), 302(1), 136-145 (2018-10-06)
Primary cilia are small organelles projecting from the cell surface of many cell types. They play a crucial role in the regulation of various signaling pathway. In this study, we investigated the importance of cilia for heart development by conditionally
Haruhiro Toko et al.
The Journal of biological chemistry, 289(9), 5348-5356 (2014-01-01)
Autologous c-kit(+) cardiac progenitor cells (CPCs) are currently used in the clinic to treat heart disease. CPC-based regeneration may be further augmented by better understanding molecular mechanisms of endogenous cardiac repair and enhancement of pro-survival signaling pathways that antagonize senescence
Loren E Dupuis et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(10), 2067-2081 (2019-08-02)
Investigate the requirement of Aggrecan (Acan) cleavage during aortic wall development in a murine model with ADAMTS (a disintegrin-like and metalloprotease domain with thrombospondin-type motifs) 5 deficiency and bicuspid aortic valves. Mice with altered extracellular matrix remodeling of proteoglycans will
Yen-Yu Lu et al.
Molecular medicine reports, 14(4), 3429-3436 (2016-08-31)
The extracellular matrix (ECM) and transforming growth factor-β (TGF)-β are important in cardiac fibrosis, however, the effects of the ECM on TGF‑β signaling remain to be fully elucidated. The aims of the present study were to evaluate the role of
S M Davidson et al.
Developmental biology, 218(2), 146-160 (2000-02-05)
The small heat-shock protein HSP25 is expressed in the heart early during development, and although multiple roles for HSP25 have been proposed, its specific role during development and differentiation is not known. P19 is an embryonal carcinoma cell line which
Shajia Lu et al.
Cell motility and the cytoskeleton, 65(9), 747-761 (2008-07-11)
We investigated the role of nonmuscle myosin heavy chain (NMHC) IIB in cultured embryonic mouse cardiomyocytes by specific knockdown using RNA interference. NMHC IIB protein levels decreased 90% compared with mock-transfected cells by 3 days post transfection. NMHC IIB knockdown
Nicolas Vignier et al.
Cell reports, 36(8), 109601-109601 (2021-08-26)
Cofilins are important for the regulation of the actin cytoskeleton, sarcomere organization, and force production. The role of cofilin-1, the non-muscle-specific isoform, in muscle function remains unclear. Mutations in LMNA encoding A-type lamins, intermediate filament proteins of the nuclear envelope
Michael D Roberts et al.
Journal of biological methods, 7(1), e127-e127 (2020-03-24)
Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determining which
Brendan Doyle et al.
Stem cells and development, 17(5), 941-951 (2008-06-20)
Administration of endothelial progenitor cells (EPC) is a promising therapy for post-infarction cardiac repair. However, the mechanisms that underlie apparent beneficial effects on myocardial remodeling are unclear. In a porcine model of acute myocardial infarction, we investigated the therapeutic effects
Federica Fontana et al.
Cell reports, 32(2), 107883-107883 (2020-07-16)
The formation of cardiac valves depends on mechanical forces exerted by blood flow. Endocardial cells lining the interior of the heart are sensitive to these stimuli and respond by rearranging into luminal cells subjected to shear stress and abluminal cells
Kate L Weeks et al.
Circulation. Heart failure, 5(4), 523-534 (2012-06-19)
Numerous molecular and biochemical changes have been linked with the cardioprotective effects of exercise, including increases in antioxidant enzymes, heat shock proteins, and regulators of cardiac myocyte proliferation. However, a master regulator of exercise-induced protection has yet to be identified.
Matthew J Brody et al.
PloS one, 7(12), e51621-e51621 (2012-12-14)
Leucine-rich repeat containing 10 (LRRC10) is a cardiac-specific protein exclusively expressed in embryonic and adult cardiomyocytes. However, the role of LRRC10 in mammalian cardiac physiology remains unknown. To determine if LRRC10 is critical for cardiac function, Lrrc10-null (Lrrc10(-/-)) mice were
Yet Hoi Hong et al.
Journal of applied physiology (Bethesda, Md. : 1985), 118(9), 1113-1121 (2015-03-10)
Inhibition of nitric oxide synthase (NOS) significantly attenuates the increase in skeletal muscle glucose uptake during contraction/exercise, and a greater attenuation is observed in individuals with Type 2 diabetes compared with healthy individuals. Therefore, NO appears to play an important
Liying Cai et al.
Stem cells (Dayton, Ohio), 27(1), 230-237 (2008-09-06)
The administration of therapeutic cell types, such as stem and progenitor cells, has gained much interest for the limitation or repair of tissue damage caused by a variety of insults. However, it is still uncertain whether the morphological and functional
David Barefield et al.
American journal of physiology. Heart and circulatory physiology, 306(6), H807-H815 (2014-01-28)
The etiology of hypertrophic cardiomyopathy (HCM) has been ascribed to mutations in genes encoding sarcomere proteins. In particular, mutations in MYBPC3, a gene which encodes cardiac myosin binding protein-C (cMyBP-C), have been implicated in over one third of HCM cases.
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