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Showing 1-30 of 42 results for "A8509" within Papers
Preparation of crystalline lithium acetoacetate.
Hall LM
Analytical Biochemistry, 3(1), 75-80 (1962)
Michael J MacDonald et al.
The Journal of biological chemistry, 286(21), 18383-18396 (2011-04-02)
Anaplerosis, the net synthesis in mitochondria of citric acid cycle intermediates, and cataplerosis, their export to the cytosol, have been shown to be important for insulin secretion in rodent beta cells. However, human islets may be different. We observed that
Konstantinos J Dabos et al.
Liver international : official journal of the International Association for the Study of the Liver, 31(5), 707-711 (2011-04-05)
Little is known about the metabolism of acetoacetate and β-hydroxybutyrate in patients with cirrhosis and encephalopathy. We investigated the fate of ketone bodies in these conditions. We studied 18 cirrhotic patients with encephalopathy and 17 cirrhotics without. At the time
Cristian Palmiere et al.
Legal medicine (Tokyo, Japan), 14(1), 17-20 (2011-12-20)
Isopropyl alcohol (IPA) is widely used as an industrial solvent and cleaning fluid. After ingestion or absorption, IPA is converted into acetone by alcohol dehydrogenase. However, in ketosis, acetone can be reduced to IPA. The aim of this study was
Justin L Rains et al.
Canadian journal of physiology and pharmacology, 90(12), 1642-1646 (2012-12-06)
Type 1 diabetic patients have hyperketonemia, elevated levels of pro-inflammatory and oxidative stress markers, and a higher incidence of vascular disease. This study examines the hypothesis that hyperketonemia increases reactive oxygen species (ROS) and is in part responsible for increased
Deana M Jaber et al.
Chemical communications (Cambridge, England), 47(27), 7623-7625 (2011-06-15)
Dirhodium catalyzed reactions of aryl-substituted tetrahydropyranone diazoacetoacetates produce ylide intermediates that unexpectedly yield two oxabicyclo[4.2.1]-nonane diastereoisomers, but a single diastereoisomer is formed by increasing the steric bulk of the aryl substituent.
Yuriko Higashino-Matsui et al.
Environmental health and preventive medicine, 17(2), 157-163 (2011-08-19)
The age-related effects of fasting on lipolysis, the production of ketone bodies, and plasma insulin levels were studied in male 3-, 8-, and 32-week-old Sprague-Dawley rats. The rats were divided into fasting and control groups. The 3-, 8- and 32-week-old
Li-Qin Xu et al.
Scientific reports, 6, 21928-21928 (2016-02-24)
The catabolism of sterols in mycobacteria is highly important due to its close relevance in the pathogenesis of pathogenic strains and the biotechnological applications of nonpathogenic strains for steroid synthesis. However, some key metabolic steps remain unknown. In this study
Rosina Pryor et al.
Cell, 178(6), 1299-1312 (2019-09-03)
Metformin is the first-line therapy for treating type 2 diabetes and a promising anti-aging drug. We set out to address the fundamental question of how gut microbes and nutrition, key regulators of host physiology, affect the effects of metformin. Combining
Carlos Henrique Dullius et al.
Applied and environmental microbiology, 77(19), 6821-6825 (2011-08-16)
A novel acetone-degrading, nitrate-reducing bacterium, strain KN Bun08, was isolated from an enrichment culture with butanone and nitrate as the sole sources of carbon and energy. The cells were motile short rods, 0.5 to 1 by 1 to 2 μm
Bei Han et al.
Applied microbiology and biotechnology, 91(3), 565-576 (2011-05-03)
Development of a butanologenic strain with high selectivity for butanol production is often proposed as a possible route for improving the economics of biobutanol production by solventogenic Clostridium species. The acetoacetate decarboxylase (aadc) gene encoding acetoacetate decarboxylase (AADC), which catalyzes
Raichel Cohen-Harazi et al.
Anticancer research, 40(7), 3831-3837 (2020-07-06)
The ketogenic diet has recently gained interest as potential adjuvant therapy for cancer. Many researchers have endeavored to support this claim in vitro. One common model utilizes treatment with exogenous acetoacetate in lithium salt form (LiAcAc). We aimed to determine
Evaggelos C Theodorou et al.
Metabolic engineering, 14(4), 354-365 (2012-04-10)
AtoSC two-component system plays a pivotal role in many regulatory indispensable Escherichia coli processes. AtoSCDAEB regulon, comprising the AtoSC system and the atoDAEB operon, regulates the short-chain fatty acids catabolism. We report here, that AtoSC up-regulates the high-molecular weight PHB
Liangcai Zhao et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 26(12), 1277-1282 (2010-12-16)
Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia, hypoinsulinemia, and ketosis. To access the biochemical process of diabetes, we applied quantitative (1)H NMR-based metabonomics to analyze urine, serum, and liver extracts from streptozotocin-induced diabetic rats. Principle component
Soo Jin Oh et al.
Chemico-biological interactions, 195(3), 173-179 (2012-01-17)
Although hepatic expression of cytochrome P450 (CYP) changes markedly in diabetes, the role of ketone bodies in the regulation of CYP in diabetes is controversial. The present study was performed to determine the expression and activity of CYP in non-obese
Mao-rong Wang et al.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, 20(5), 377-380 (2012-09-14)
To investigate the inhibitory effect of acetoacetate extract from Celastrus orbiculatus Thumb (COT) on the growth of red fluorescent protein (RFP)-xenografted human hepatocellular carcinoma (HCC) in a nude mouse model. Human HCC HepG2 cells were transduced with RFP and inoculated
Jordan M Wilkins et al.
Aging, 12(14), 15134-15156 (2020-07-09)
Multiple sclerosis (MS) is a central nervous system inflammatory demyelinating disease and the most common cause of non-traumatic disability in young adults. Despite progress in the treatment of the active relapsing disease, therapeutic options targeting irreversible progressive decline remain limited.
Marina C Theodorou et al.
Amino acids, 43(2), 833-844 (2011-11-16)
The AtoSC two-component system in Escherichia coli is a key regulator of many physiological processes. We report here the contribution of AtoSC in E. coli motility and chemotaxis. AtoSC locus deletion in ΔatoSC cells renders cells not motile or responsive
Trudi Denoon et al.
Toxicology letters, 323, 19-24 (2020-01-22)
Cultured kidney cells maintained in conventional growth media with high glucose levels exhibit increased glycolytic activity compared to the cells in vivo. In contrast, renal proximal tubules utilize substrates such as ketone bodies and rely on mitochondrial oxidative phosphorylation. LLC-PK1
Alexandre Courchesne-Loyer et al.
Nutrition (Burbank, Los Angeles County, Calif.), 29(4), 635-640 (2013-01-01)
In humans consuming a normal diet, we investigated 1) the capacity of a medium-chain triacylglycerol (MCT) supplement to stimulate and sustain ketonemia, 2) ¹³C-β-hydroxybutyrate and ¹³C-trioctanoate metabolism, and 3) the theoretical contribution of the degree of ketonemia achieved to brain
Michael J McAnulty et al.
Biotechnology and bioengineering, 114(4), 852-861 (2016-11-02)
We previously demonstrated anaerobic conversion of the greenhouse gas methane into acetate using an engineered archaeon that produces methyl-coenzyme M reductase (Mcr) from unculturable microorganisms from a microbial mat in the Black Sea to create the first culturable prokaryote that
J L Hansen et al.
Clinical chemistry, 24(3), 475-479 (1978-03-01)
Methods are described for direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate in plasma with the GEMSAEC centrifugal analyzer. The methods for lactate, beta-hydroxybutyrate, and acetoacetate are kinetic and ratiometric, eliminating the need for specimen-blank assays. The pyruvate method is
Dalibor Kurepa et al.
Neonatology, 102(3), 163-168 (2012-07-11)
Infants of diabetic mothers (IDMs) are at increased risk for metabolic complications. Type 1 and some type 2 diabetic patients have elevated levels of the ketone bodies acetoacetate (AA) and β-hydroxybutyrate (BHB). The aim of this study was to examine
Rami Al Batran et al.
Cell metabolism, 31(5), 909-919 (2020-04-11)
Perturbations in carbohydrate, lipid, and protein metabolism contribute to obesity-induced type 2 diabetes (T2D), though whether alterations in ketone body metabolism influence T2D pathology is unknown. We report here that activity of the rate-limiting enzyme for ketone body oxidation, succinyl-CoA:3-ketoacid-CoA
Seppo Parkkila et al.
Chemical communications (Cambridge, England), 48(29), 3551-3553 (2012-03-03)
Acetoacetic acid and R-3-hydroxy-butyric acid (BHB) are "ketone bodies", metabolites produced during the ketogenic diet. We discovered that they inhibit in the submicromolar-micromolar range several carbonic anhydrase (CA, EC isoforms involved in relevant physiologic processes such as lipogenesis and
Prashanth Srinivasan et al.
Nature communications, 10(1), 3634-3634 (2019-08-14)
Tropane alkaloids (TAs) are a class of phytochemicals produced by plants of the nightshade family used for treating diverse neurological disorders. Here, we demonstrate de novo production of tropine, a key intermediate in the biosynthetic pathway of medicinal TAs such
Ángel Sánchez-Illana et al.
Scientific reports, 7(1), 17039-17039 (2017-12-08)
Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying
Tina D Shah et al.
Molecular and biochemical parasitology, 179(2), 100-106 (2011-07-20)
A putative β-hydroxybutyrate dehydrogenase (βHBDH) ortholog was identified in Trypanosoma brucei, the unicellular eukaryotic parasite responsible for causing African Sleeping Sickness. The trypanosome enzyme has greater sequence similarity to bacterial sources of soluble βHBDH than to membrane-bound Type I βHBDH
W T Hron et al.
Journal of lipid research, 22(2), 377-381 (1981-02-01)
A sensitive micromethod for the colorimetric determination of free fatty acids (FFA) in plasma has been developed. FFA are extracted with chloroform-heptane-methanol with silicic acid being added to eliminate interfering phospholipids. Diphenylcarbazide containing diphenylcarbazone has been chosen as color-developing reagent
Evaggelos C Theodorou et al.
Biochimica et biophysica acta, 1810(5), 561-568 (2011-02-08)
We have shown previously that AtoSC two-component system regulates the biosynthesis of E. coli cPHB [complexed poly-(R)-3-hydroxybutyrate]. The AtoSC involvement on fatty acids metabolism, towards cPHB synthesis, was studied using cPHB determination, gene expression, and fatty acid metabolic pathways inhibitors.
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