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Showing 1-30 of 74 results for "A8549" within Papers
Jialei Tang et al.
Scientific reports, 7(1), 10945-10945 (2017-09-10)
We report a simple single-molecule fluorescence imaging method that increases the temporal resolution of any type of array detector by >5-fold with full field-of-view. We spread single-molecule spots to adjacent pixels by rotating a mirror in the detection path during
Narayanan Madaboosi et al.
The Analyst, 140(13), 4423-4433 (2015-05-20)
As a leading cause of cancer-related deaths in men globally, prostate cancer (PCa) demands immense attention for theranostic purposes. There is an increasing need for the development of rapid, sensitive, economical, miniaturized and multiplexable assays. Towards this goal, we present
Susanne C M Reinhardt et al.
Nature, 617(7962), 711-716 (2023-05-25)
Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in the life sciences to understand complex biological systems. Super-resolution approaches1-6 can achieve resolution in cells in the range of 15 to 20ā€‰nm, but interactions between
Yongliang Wang et al.
Biosensors & bioelectronics, 100, 192-200 (2017-09-16)
Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution
Florian Schueder et al.
Nature communications, 8(1), 2090-2090 (2017-12-14)
Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC)
Yongliang Wang et al.
Journal of biophotonics, 12(5), e201800351-e201800351 (2018-11-30)
Nucleases are important enzymes that cleave nucleic acids and play critical roles in DNA repair, immune defense and potentially in cancer invasion. However, their spatial dynamics at subcellular level is much less studied. Here, we developed a surface-tethered nuclease sensor
Konstantinos Nakos et al.
Cytoskeleton (Hoboken, N.J.), 76(1), 83-91 (2018-08-26)
Septins are GTP-binding proteins that associate with the microtubule (MT) and actin cytoskeleton. Septins affect MT organization and posttranslational modifications, but their role in MT dynamics is less understood. Here, we reconstituted MT dynamics in the presence of the MT-binding
Stijn van Dorp et al.
eLife, 10 (2021-11-04)
The dimeric ER Ca2+ sensor STIM1 controls store-operated Ca2+ entry (SOCE) through the regulated binding of its CRAC activation domain (CAD) to Orai channels in the plasma membrane. In resting cells, the STIM1 CC1 domain interacts with CAD to suppress
Burak Okumus et al.
Methods in molecular biology (Clifton, N.J.), 608, 81-96 (2009-12-17)
The potential importance of G-quadruplex structures was implied by the recent findings that the human POT1 disrupts G-quadruplex and stimulates the telomerase activity. A solid understanding of the range of conformations that can be adopted by guanine-rich sequences can potentially
Marc Zinggeler et al.
Scientific reports, 9(1), 1246-1246 (2019-02-06)
Circulating tumor cells (CTCs) play a key role during the metastatic process of human cancers and their reliable detection and characterization could enable new and effective ways of cancer diagnosis, monitoring and treatment. However, due to their ultralow concentration in
Taikopaul Kaneko et al.
Science advances, 6(4), eaax7413-eaax7413 (2020-02-06)
Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin.
Taketoshi Kajimoto et al.
Bio-protocol, 8(24), e3111-e3111 (2018-12-20)
Function of extracellular vesicles such as exosomes and microvesicles is determined by their wide ranges of cargoes inside them. Even in the pure exosomes or microvesicles the cargo contents are very heterogeneous. To understand this heterogeneous nature of extracellular vesicles
Yuanchang Zhao et al.
Biosensors & bioelectronics, 150, 111959-111959 (2020-01-14)
DNA is a versatile biomaterial with well-defined mechanical and biochemical properties. It has been broadly adopted to synthesize tension sensors that calibrate and visualize cellular forces at the cell-matrix interface. Here we showed that DNA-based tension sensors are vulnerable to
Dongyu Li et al.
Microbiology and immunology, 64(11), 768-777 (2020-09-10)
The Gram-negative bacterial pathogen Yersinia delivers six effector proteins into the host cells to block the host innate immune response. One of the effectors, YopT, is a potent cysteine protease that causes the disruption of the actin cytoskeleton to inhibit
Priyanka Agarwal et al.
ACS measurement science au, 2(4), 317-324 (2023-02-15)
Factors that affect the performance of the nucleic acid lateral flow assay (NALFA) have not been well studied. In this work, we identify two important phenomena that negatively affect signal intensities during the detection of PCR products using NALFA: (i)
Parastoo Maleki et al.
Molecular genetics and genomics : MGG, 292(3), 483-498 (2017-02-06)
In this article, we summarize the knowledge and best practices learned from bulk and single-molecule measurements to address some of the frequently experienced difficulties in single-molecule Fƶrster resonance energy transfer (smFRET) measurements on G-quadruplex (GQ) structures. The number of studies
Jan Christoph Thiele et al.
Science advances, 8(23), eabo2506-eabo2506 (2022-06-09)
Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution, which is by a factor of 2 to
Samuel W Schaffter et al.
Nature communications, 11(1), 6057-6057 (2020-11-29)
Crystallization is a ubiquitous means of self-assembly that can organize matter over length scales orders of magnitude larger than those of the monomer units. Yet crystallization is notoriously difficult to control because it is exquisitely sensitive to monomer concentration, which
Simao Coelho et al.
Nature protocols, 16(1), 497-515 (2020-12-04)
A key part of any super-resolution technique involves accurately correcting for mechanical motion of the sample and setup during acquisition. If left uncorrected, drift degrades the resolution of the final reconstructed image and can introduce unwanted artifacts. Here, we describe
Joschka Hellmeier et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(4) (2021-01-21)
T cells detect with their T cell antigen receptors (TCRs) the presence of rare agonist peptide/MHC complexes (pMHCs) on the surface of antigen-presenting cells (APCs). How extracellular ligand binding triggers intracellular signaling is poorly understood, yet spatial antigen arrangement on
Simon Hennig et al.
ACS nano, 9(8), 8122-8130 (2015-07-15)
We describe a method for the deposition of minute amounts of fluorophore-labeled oligonucleotides with high local precision in conductive and transparent solid layers of poly(vinyl alcohol) (PVA) doped with glycerin and cysteamine (PVA-G-C layers). Deposition of negatively charged fluorescent molecules
Mark Bates et al.
Nature methods, 19(5), 603-612 (2022-05-17)
Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10ā€‰nm spatial resolution in three dimensions. Despite its outstanding sensitivity, wider application of this technique has been hindered by complex instrumentation and the challenging nature of
A Robin Temming et al.
Frontiers in immunology, 12, 594773-594773 (2021-04-02)
Antibody-mediated blood disorders ensue after auto- or alloimmunization against blood cell antigens, resulting in cytopenia. Although the mechanisms of cell destruction are the same as in immunotherapies targeting tumor cells, many factors are still unknown. Antibody titers, for example, often
Francesca Zuttion et al.
Nature communications, 11(1), 6312-6312 (2020-12-11)
The increase in speed of the high-speed atomic force microscopy (HS-AFM) compared to that of the conventional AFM made possible the first-ever visualisation at the molecular-level of the activity of an antimicrobial peptide on a membrane. We investigated the medically
Hamidreza Heydarian et al.
Nature methods, 15(10), 781-784 (2018-09-19)
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration
Tamara Heermann et al.
Nature methods, 18(10), 1239-1246 (2021-10-06)
In spite of their great importance in biology, methods providing access to spontaneous molecular interactions with and on biological membranes have been sparse. The recent advent of mass photometry to quantify mass distributions of unlabeled biomolecules landing on surfaces raised
Xiaolin Wang et al.
Frontiers in cellular and infection microbiology, 10, 511798-511798 (2020-10-30)
Activation of the NLRP3 inflammasome requires the expression of NLRP3, which is strictly regulated by its capacity to directly recognize microbial-derived substances. Even though the involvement of caspase-1 activation in macrophages via NLRP3 and NLRC4 has been discovered, the accurate
Kaushik Pal et al.
The Journal of cell biology, 220(7) (2021-04-28)
Podosomes and invadopodia, collectively termed invadosomes, are adhesive and degradative membrane structures formed in many types of cells and are well known for recruiting various proteases. However, another major class of degradative enzymes, deoxyribonuclease (DNase), remains unconfirmed and not studied
Elaine Ng et al.
Sensors and actuators. B, Chemical, 387, 133773-133773 (2023-04-15)
The COVID-19 pandemic has highlighted the need to monitor important correlates of immunity on a population-wide level. To this end, we have developed a competitive assay to assess neutralizing antibody (NAb) titer on the giant magnetoresistive (GMR) biosensor platform. We
Diqing Su et al.
Frontiers in microbiology, 10, 1077-1077 (2019-06-06)
The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of
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