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alcian Blue

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Showing 1-30 of 538 results for "alcian Blue" within Papers
Florian Ponzio et al.
Journal of colloid and interface science, 459, 29-35 (2015-08-12)
Polydopamine (PDA) coatings appear as a universal functionalization methodology allowing to coat the surface of almost all kinds of known materials with a conformal, stable, robust and reactive material. Relatively few investigations were dedicated to the incorporation of other molecules
Nguyen Thi Thuy et al.
Chemosphere, 131, 139-148 (2015-04-04)
This paper reports on the fate of different fractions of Alcian blue (AB) stainable material in Pao-Shan reservoir, Taiwan, in a one-year study (2013-2014) and an intensive study during phytoplankton bloom (2014). The interactions between the fractions, including AB stained
Dennis R Johnson et al.
World journal of gastroenterology, 21(9), 2770-2776 (2015-03-12)
To compare the sensitivity and specificity of CDX2 and alcian blue (AB) pH 2.5 staining in identifying esophageal intestinal metaplasia. One hundred and ninty-nine biopsies from 186 patients were retrospectively reviewed and categorized as Barrett's esophagus (BE) (n = 108);
R S Wall et al.
Analytical biochemistry, 175(1), 298-299 (1988-11-15)
A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background
C J Churukian et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 147-150 (2000-08-19)
We compared the staining performance, dye content, solubility, and visual absorption maximum of two batches of alcian blue pyridine variant and of five batches of alcian blue 8GX (C.I. 74240). Whenever possible, we also compared results to those obtained with
R L Ridgway
Comparative biochemistry and physiology. A, Comparative physiology, 87(2), 295-303 (1987-01-01)
1. The neurosecretory system of the primitive ellobiid Melampus bidentatus (Pulmonata: Basommatophora) was investigated using Alcian blue-Alcian yellow histochemistry. 2. Putative neurosecretory cells within the central ganglia were distinguished by the criteria of cell size, position, and staining reaction. 3.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
Weijie Dong et al.
Analytical chemistry, 84(20), 8461-8466 (2012-09-07)
The isolation and characterization of mucins are critically important for obtaining insight into the molecular pathology of various diseases, including cancers and cystic fibrosis. Recently, we developed a novel membrane electrophoretic method, supported molecular matrix electrophoresis (SMME), which separates mucins
Anca Florentina Caliman et al.
Journal of hazardous materials, 144(1-2), 265-273 (2006-11-23)
The photocatalytic degradation of Alcian Blue 8 GX, a cationic copper phthalocyanine dye, has been investigated in aqueous suspensions containing the commercial catalyst TiO(2) P-25. The photodegradation of the organic molecule follows approximately a pseudo-first kinetic order, according to the
D K Meyerholz et al.
Veterinary pathology, 46(2), 325-328 (2009-03-06)
Utilization of a combined Alcian Blue and Pyronine Y histochemical method for the assessment of multiple parameters in the respiratory tract of various species is described. Acidic mucins were deep blue (sialylated mucins), red (sulfated mucins), or variably purple (mixture
Schachner et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 76(1), 15-22 (2001-07-07)
The staining method developed by Christian Gram was introduced as a simple and highly selective tool for demonstrating myxosporean and coccidian sporogonic stages. When using standard blood staining procedures for those enigmatic parasites it is sometimes difficult to distinguish them
E T Graham et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 73(4), 178-185 (1998-09-15)
A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer, pH 5.0. Paraffin was extracted with MicroClear solvent from 10 microm tissue sections mounted on slides. Paraffin solvent was
J E Scott
European journal of oral sciences, 104(1), 2-9 (1996-02-01)
A brief outline of the history and chemistry of the copper pthalocyanin-based Alcian blue dyes highlights the adverse effects of commercial secrecy and entrepreneurial dishonesty on the use of these valuable histochemical and biochemical reagents in the biological sciences. The
E V Isaeva et al.
Sovremennye tekhnologii v meditsine, 15(2), 5-16 (2023-06-30)
The aim of the study was to compare type I collagen-based and methacryloyl gelatin-based (GelMA) hydrogels by their ability to form hyaline cartilage in animals after subcutaneous implantation of scaffolds. Chondrocytes were isolated from the costal cartilage of newborn rats
V H Tomasi et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 78(5), 255-259 (2004-03-03)
We describe here a new method for specific staining of mast cells using ferroin. Different hamster tissues were fixed in 4% formalin and processed for paraffin embedding. Sections were stained with hematoxylin followed by ferroin acidified with 2.5 N sulfuric
Giovanni Candiano et al.
Analytical chemistry, 87(9), 4814-4820 (2015-04-10)
Combinatorial peptide ligand libraries (CPLLs) tend to bind complex molecules such as dyes due to their aromatic, heterocyclic, hydrophobic, and ionic nature that may affect the protein capture specificity. In this experimental work Alcian Blue 8GX, a positively charged phthalocyanine
J Van Minnen et al.
Journal of neurobiology, 12(3), 297-301 (1981-05-01)
The neurosecretory system of the giant garden slug Limax maximus was studied using the alcian blue/alcian yellow (AB/AY) staining technique for neurosecretion. Stainable cells could be identified in the paired cerebral, pleural, parietal, and buccal ganglia, and in the visceral
W Hong et al.
Journal of microbiological methods, 42(2), 121-127 (2000-10-06)
Since a method of contrast enhancement for immunoelectron microscopy has not been available in bacteriology, the morphological localization of proteins of Helicobacter pylori is not well known. In this report, we established a method of contrast enhancement in immunoelectron microscopy
E M Center et al.
Histology and histopathology, 7(2), 231-236 (1992-04-01)
The etiology of the eye defects in myelencephalic blebs (my) mutant mice has been poorly understood for almost seventy years. Embryos from 9 to 14 1/2 days of gestation were subjected to Alcian blue 8GX staining for acidic glycosaminoglycan deposition
Correspondence: Chromotrope 2R.
Bernard M Wagner
Toxicologic pathology, 31(6), 717-717 (2003-10-31)
Cellulose acetate electrophoresis of glycosaminoglycans.
Y Wegrowski et al.
Methods in molecular biology (Clifton, N.J.), 171, 175-179 (2001-07-14)
Loreen O Villacorte et al.
Water research, 70, 300-312 (2014-12-30)
Transparent exopolymer particles (TEP) and their precursors produced by phyto-/bacterio-planktons in fresh and marine aquatic environments are increasingly considered as a major contributor to organic/particulate and biological fouling in micro-/ultra-filtration and reverse osmosis membrane (RO) systems. However, currently established methods
Emily B Martin et al.
Biochemical and biophysical research communications, 436(1), 85-89 (2013-05-28)
In previously published work, we have described heparin-binding synthetic peptides that preferentially recognize amyloid deposits in a mouse model of reactive systemic (AA) amyloidosis and can be imaged by using positron and single photon emission tomographic imaging. We wanted to
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
A A Smith et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 86(2), 98-103 (2009-12-17)
Re-staining of formalin fixed paraffin sections sometimes is required and this requires prior de-staining. Some simple and effective protocols for de-staining are described. Mucihematoxylin and mucicarmine can be removed with acid alcohol. Zirconyl hematoxylin can be removed with periodic acid
D E Terry et al.
Analytical biochemistry, 285(2), 211-219 (2000-10-06)
Alcian blue and toluidine blue dyes form complexes with anionic glycoconjugates (AG) such as proteoglycans (PG) and glycosaminoglycans (GAG). However, the Alcian blue-AG complexes do not readily dissociate, while the toluidine blue-AG complexes do so in salt solutions. This differential
Désirée Schatton et al.
eLife, 11 (2022-05-14)
Proliferating cells undergo metabolic changes in synchrony with cell cycle progression and cell division. Mitochondria provide fuel, metabolites, and ATP during different phases of the cell cycle, however it is not completely understood how mitochondrial function and the cell cycle
J J Abramson et al.
Archives of biochemistry and biophysics, 263(2), 245-255 (1988-06-01)
The copper containing phthalocyanine dyes, alcian blue, copper phthalocyanine tetrasulfonic acid, and Luxol fast blue MBSN are found to induce rapid calcium efflux from actively loaded sarcoplasmic reticulum (SR) vesicles. Alcian blue (5 microM), with 1 mM free Mg2+ triggered
K Sugimoto et al.
Journal of neurochemistry, 65(2), 791-797 (1995-08-01)
The mast cell-deficient [Ws/Ws (White spotting in the skin)] rat was investigated with regard to the origin of histamine in the brain. No mast cells were detected in the pia mater and the perivascular region of the thalamus of Ws/Ws
J R Garrett et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(5), 541-547 (1987-05-01)
Proteases present in mast cell granules have been harnessed to demonstrate mast cells in human tissues. A number of substrate mixtures were tested. D-Val-Leu-Arg-4-methoxy-2-naphthylamide (MNA) plus Fast Blue B was the best for identifying human mast cells, yielding the most
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