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SIGMAFAST BCIP/NBT tablet (B5655)
SIGMAFAST BCIP/NBT tablet (B5655) Datasheet
SIGMAFAST BCIP/NBT tablet
Catalog Number B5655
Storage Temperature –20 °C
Product Description
SIGMAFAST BCIP/NBT (5-bromo-4-chloro-3-indolyl
Data Sheet - B5655
08/22
1
Product Information
SIGMAFAST™ BCIP®/NBT Tablet
Data Sheet - A3187
1:30,000 by direct dot blot against 1 mg human IgG
using BCIP/NBT, SIGMAFAST Tablets (Cat. No.
B5655) as substrate.
1:50 By Immunoblotting against formalin-fixed, paraffin-
embedded human tonsil sections
Data Sheet - A5187
Bromo-4-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTM Tablets, Cat. No. B5655.
Western Blotting: Minimum 1:30,000
Sheep IgG was detected directly using 10 µg protein
per lane
Data Sheet - A3687
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTM Tablets, Catalog Number B5655.
Direct ELISA: minimum titer 1:30,000
Titer is defined as the dilution of conjugate sufficient
Data Sheet - A4937
-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTablets, Catalog Number B5655.
Direct ELISA: Minimum 1:30,000
Titer is defined as the dilution of conjugate sufficient to
give
Data Sheet - A1192
chloro-3-indolylphosphate/
nitroblue tetrazolium (BCIP/NBT), SIGMAFASTTM
Tablets, Catalog Number B5655.
Direct ELISA: minimum titer 1:35,000
Titer is defined as the dilution of conjugate sufficient
Data Sheet - A3063 - Lot 067H8970
5-Bromo-4-chloro-3-indolyl Phosphate/
Nitroblue Tetrazolium (BCIP/NBT, SIGMA FASTTM
Tablets, B5655).
3. Immunohistology: 1:50
Determined by a direct assay using formalin-fixed,
paraffin-embedded
Data Sheet - A3437
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Catalog Number B5655.
Direct Immunohistochemistry: a minimum titer of 1:50 is
determined by a direct assay using formalin-fixed
Data Sheet - A3562
-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTablets, Catalog Number B5655.
Direct ELISA: a minimum titer of 1:30,000 is determined
Titer is defined as the dilution of conjugate
Data Sheet - A1067
5-Bromo-4-chloro-3-indolyl Phosphate/
Nitroblue Tetrazolium (BCIP/NBT, SIGMA FASTTM
Tablets, B5655).
3. Immunohistology: 1:100
Determined by a direct assay using formalin-fixed,
paraffin-embedded
Data Sheet - A3688
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTM Tablets, Catalog Number B5655.
Immunohistology: minimum 1:50
Determined by an indirect assay using formalin-fixed,
paraffin-
Data Sheet - A3812
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Catalog Number B5655.
Immunohistology: minimum titer of 1:50
Determined by an indirect assay using formalin-fixed
Data Sheet - A4312 - Lot 087H9171
5-Bromo-4-chloro-3-indolyl Phosphate/
Nitroblue Tetrazolium (BCIP/NBT, SIGMA FASTTM
Tablets, B5655).
3. Immunohistology: 1:100
Determined by an indirect assay using formalin-fixed,
paraffin-
Data Sheet - A5062
Bromo-4-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Cat. No. B5655.
Immunohistology
The minimum working antibody dilution of 1:50 was
determined by an indirect assay
Data Sheet - A3438
chloro-3-indolyl
phosphate/Nitroblue tetrazolium (BCIP/NBT),
SIGMAFASTTM Tablets, Catalog Number B5655.
Immunohistochemistry: Minimum 1:50
Determined by an indirect assay using formalin-fixed,
paraffin-embedded
Data Sheet - A3312
bromo-4-chloro-3-indolyl Phosphate/-
Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTablets, Cat. No. B5655
Immunohistology: Minimum 1:50
Determined by a direct assay using formalin-fixed,
paraffin-embedded
Data Sheet - A3563
-chloro-3-indolyl phosphate/Nitro
Blue Tetrazolium, SIGMAFAST BCIP/NBT Tablets,
Catalog Number B5655.
Direct ELISA: recommended working dilution 1:30,000
Multiwell plates are coated with purified
Data Sheet - A9316
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTM Tablets, Catalog Number B5655.
Immunohistochemistry: minimum 1:50
Determined by an indirect assay using formalin-fixed,
paraffin-embedded
Data Sheet - A0705
Bromo-4-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT), SIGMA
FASTTM Tablets, Cat. No. B5655.
Direct ELISA: titer 1:30,000
Titer is defined as the dilution of conjugate sufficient to
give
Data Sheet - A6063
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST™ Tablets, Catalog Number B5655.
Note: Working dilutions should be determined by
titration assays. Due to differences in assay
Data Sheet - A4062
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Catalog Number B5655.
Immunohistochemistry: a minimum working dilution of
1:50 is determined by indirect immunohistochemistry
Data Sheet - A7064
Substrate: 5-Bromo-4-chloro-3-idolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT,
SIGMA FASTTM Tablets, B5655).
3. Immunohistology: Minimum 1:50
Determined by an indirect assay
Data Sheet - A8438
chloro-3-indolyl Phosphate/
Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFASTTablets, Catalog Number B5655.
Direct ELISA: minimum titer 1:30,000
Titer is defined as the dilution of conjugate sufficient
Data Sheet - A3937
chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Catalog Number B5655.
Immunohistochemistry: Minimum 1:50
Determined by an indirect assay using formalin-fixed,
paraffin-embedded
Data Sheet - A3188
Bromo-4-chloro-3-indolyl
phosphate/nitroblue tetrazolium, SIGMA FASTTM
BCIP/NBT tablets, Cat. No. B5655.
Immunohistochemistry: minimum dilution 1:50
Determined by a direct assay using formalin-fixed
Data Sheet - A3813
Bromo-4-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Cat. No. B5655.
Immunohistolochemistry: minimum antibody dilution
1:50
Determined by a direct assay using formalin-fixed
Data Sheet - A4187
-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT) SIGMA
FAST Tablets, Catalog Number B5655.
Note: Working dilutions should be determined by titra-
tion assays. Due to differences in assay
Data Sheet - A4312
-chloro-3-indolyl
Phosphate/Nitroblue Tetrazolium (BCIP/NBT),
SIGMAFAST Tablets, Catalog Number B5655.
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