CLS430909SIGMA

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Showing 1-18 of 18 results for "CLS430909SIGMA" within Site Content

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Reverse Transcription Protocol Using SYBR Green Dye Detection

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.

Multiplex qPCR Protocol

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

qPCR with Single Detection Probe

qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.

Standard Reverse Transcription Protocol

Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.

Primer Concentration Optimization

Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.

Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Primer Optimization Using Temperature Gradient

Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.

qPCR Gene Expression Protocol Using SYBR Green

SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

qPCR Efficiency Determination Protocol

Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

SPUD Assay for Detection of Assay Inhibitors Protocol

SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions