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Showing 1-30 of 31 results for "D38308" within Papers
Daniela P Marchi-Salvador et al.
Biochimica et biophysica acta, 1794(11), 1583-1590 (2009-07-21)
For the first time, the structure of a catalytic inactive phospholipase A(2) homolog (Lys49-PLA(2)s) complexed with p-bromophenacyl bromide (BPB) has been solved by X-ray crystallography. Lys49-PLA(2)s are among the main components of Viperidae snake venoms, causing myonecrosis and other actions
Jordan Doumanov et al.
Toxicon : official journal of the International Society on Toxinology, 94, 36-44 (2014-12-24)
Snake venom Phospholipases A2 (svPLA2) are among the main toxic venom components with a great impact on different tissues and organs based on their catalytic specificity and a variety of pharmacological effects, whose mechanism is still under debate. The main
Lei Wang et al.
Applied microbiology and biotechnology, 75(5), 1047-1053 (2007-04-03)
Pseudomonas putida GP01 cells that had accumulated medium-chain-length polyhydroxyalkanoates (PHA(MCL)) secreted 3-hydroxyoctanoate and 3-hydroxyhexanoate when incubated in alkaline buffers. The release of acids strongly decreased the pH resulting in less efficient secretion of 3HA(MCL) at neutral pH. To increase the
Rodrigo G Stábeli et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 142(3-4), 371-381 (2006-01-31)
MjTX-II, a myotoxic phospholipase A(2) (PLA(2)) homologue from Bothrops moojeni venom, was functionally and structurally characterized. The MjTX-II characterization included: (i) functional characterization (antitumoral, antimicrobial and antiparasitic effects); (ii) effects of structural modifications by 4-bromophenacyl bromide (BPB), cyanogen bromide (CNBr)
Tetrahedron Letters, 34, 4547-4547 (1993)
Synthetic Communications, 23, 271-271 (1993)
Adriana G Martins et al.
Molecular and cellular biochemistry, 286(1-2), 115-124 (2006-05-03)
In the rat liver NAD+ infusion produces increases in portal perfusion pressure and glycogenolysis and transient inhibition of oxygen consumption. The aim of the present work was to investigate the possible action of this agent on gluconeogenesis using lactate as
Sony Shrestha et al.
Journal of microbiology (Seoul, Korea), 47(6), 774-782 (2010-02-04)
An entomopathogenic bacterium, Xenorhabdus nematophila, induces an immunosuppression of target insects by inhibiting phospholipase A(2) (PLA(2)) activity. Recently, an immune-associated PLA(2) gene was identified from the red flour beetle, Tribolium castaneum. This study cloned this PLA(2) gene in a bacterial
Youngjin Park et al.
Archives of insect biochemistry and physiology, 60(3), 105-115 (2005-10-20)
We report on a secretory phospholipase A2 (sPLA2) associated with membrane-enriched fractions prepared from hemocytes of the tobacco hornworms, Manduca sexta. Virtually no PLA2 activity was detected in serum of immunologically naive or bacterially challenged hornworms. PLA2 activity was detected
Sandeep Ravindran et al.
PloS one, 4(12), e8143-e8143 (2009-12-04)
Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa that is able to infect a wide variety of host cells. During its active invasion process it secretes proteins from discrete secretory organelles: the micronemes, rhoptries and dense granules. Although
He-ying Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 893-894, 49-56 (2012-03-21)
A comparison of three derivatization reagents (dansyl chloride, diazomethane and p-bromophenacyl bromide) for the simultaneous quantitation of three anticancer chemicals (tegafur, 5-fluorouracil and gimeracil) and endogenous uracil in plasma using high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed
Markus Berger et al.
Journal of thrombosis and thrombolysis, 30(3), 300-310 (2010-02-17)
The caterpillar Lonomia obliqua is a venomous animal that causes numerous accidents, especially in southern Brazil, where it is considered a public health problem. The clinical manifestations include several haemostatic disturbances that lead to a hemorrhagic syndrome. Considering that platelets
Daniel I Perez et al.
Journal of medicinal chemistry, 54(12), 4042-4056 (2011-04-20)
Development of kinase-targeted therapies for central nervous system (CNS) diseases is a great challenge. Glycogen synthase kinase 3 (GSK-3) offers a great potential for severe CNS unmet diseases, being one of the inhibitors on clinical trials for different tauopathies. Following
Wan Chen et al.
Thrombosis and haemostasis, 112(4), 700-715 (2014-07-11)
Snake venoms are excellent sources of pharmacologically active proteins and peptides, and hence are potential sources of leads for drug developments. It has been previously established that krait (Bungarus genus) venoms contain mainly neurotoxins. A screening for anticoagulants showed that
Mohammad Tariq et al.
World journal of gastroenterology, 12(36), 5798-5804 (2006-09-29)
To study the effect of bromophenacyl bromide (BPB), a phospholipase A2 inhibitor on gastric secretion and to protect chemically induced gastric and duodenal ulcers in rats. Acid secretion studies were undertaken in pylorus-ligated rats with BPB treatment (0, 5, 15
Mark Merchant et al.
Journal of experimental zoology. Part A, Ecological genetics and physiology, 311(9), 662-666 (2009-07-31)
PLA(2) is a diverse class of enzymes with a broad spectrum of physiological functions. Secretory PLA(2) isoforms have been reported to exhibit important innate immune function in higher vertebrates. This study was conducted to characterize PLA(2) activity in the serum
Wen-Hsin Liu et al.
Toxicology letters, 185(2), 102-109 (2009-01-03)
In view of the controversial role of catalytic activity on the cytotoxicity of phospholipase A(2) (PLA(2)), the present study is conducted to explore whether PLA(2) induces apoptotic process of human leukemia U937 cells through catalytic activity-independent pathway. Modification of His-48
Christopher L Sprague et al.
Toxicology and applied pharmacology, 207(3), 266-274 (2005-09-01)
3-Butene-1,2-diol (BDD), an allylic alcohol and major metabolite of 1,3-butadiene, has previously been shown to cause hepatotoxicity and hypoglycemia in male Sprague-Dawley rats, but the mechanisms of toxicity were unclear. In this study, rats were administered BDD (250 mg/kg) or
M V Egorova et al.
Bulletin of experimental biology and medicine, 146(6), 695-697 (2009-06-11)
The rate of oxygen consumption by isolated cardiomyocytes was studied in rats with experimental postinfarction cardiosclerosis. The increase in oxygen consumption under these condition was comparable to that in melittin- and arachidonic acid-induced activation of phospholipase A2 in cardiomyocytes of
Sanjaya Kuruppu et al.
The Journal of pharmacology and experimental therapeutics, 315(3), 1196-1202 (2005-09-02)
The Papuan taipan (Oxyuranus scutellatus canni) is widely distributed throughout much of Papua New Guinea. Although neurotoxicity is a major symptom of envenomation, no neurotoxins have been isolated from this venom. Using a series of size exclusion chromatography steps, we
J Lane et al.
Toxicon : official journal of the International Society on Toxinology, 58(3), 239-246 (2011-07-05)
The coagulant effects of Australasian black snakes (Pseudechis spp.) are poorly understood and differ to the procoagulant venoms of most dangerous snakes in Australia. This study aimed to investigate in vitro coagulant effects of Pseudechis venoms and the efficacy of commercial
Xiao-Wang Qu et al.
Biochemical and biophysical research communications, 365(1), 158-163 (2007-11-06)
Human bocavirus (HBoV) is a new parvovirus first discovered in 2005, which is associated with acute respiratory infection. Analysis of sequence homology has revealed that a putative phospholipase A2 (PLA2) motif exists in the VP1 unique region of HBoV. However
Johanna Mansfeld et al.
Chemistry and physics of lipids, 150(2), 156-166 (2007-08-19)
The secretory phospholipase A2-alpha from Arabidopsis thaliana (AtsPLA2-alpha), being one of the first plant sPLA2s obtained in purified state, has been characterised with respect to substrate preference and optimum conditions of catalysis. The optima of pH, temperature, and calcium concentration
D P Marchi-Salvador et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 6), 600-603 (2006-06-07)
For the first time, a non-catalytic and myotoxic Lys49-PLA2 (BthTX-I from Bothrops jararacussu venom) has been crystallized with BPB inhibitor. X-ray diffraction data were collected and electron-density calculations showed that the ligand is bound to the His48 residue. BthTX-I with
Tetrahedron, 49, 9735-9735 (1993)
Karinne Cristinne da Silva Cunha et al.
Toxicon : official journal of the International Society on Toxinology, 57(4), 580-585 (2011-01-13)
We have previously showed that a phospholipase A₂ isolated from Lachesis muta snake venom and named LM-PLA₂-I displayed particular biological activities, as hemolysis, inhibition on platelet aggregation, edema induction and myotoxicity. In the present work, we evaluated the effect of
Rafael M Ximenes et al.
BMC complementary and alternative medicine, 12, 139-139 (2012-08-29)
Harpalycin 2 (HP-2) is an isoflavone isolated from the leaves of Harpalyce brasiliana Benth., a snakeroot found in northeast region of Brazil and used in folk medicine to treat snakebite. Its leaves are said to be anti-inflammatory. Secretory phospholipases A2
Heiki Vija et al.
Toxicon : official journal of the International Society on Toxinology, 54(4), 429-439 (2009-05-27)
Two novel acidic phospholipase A(2)s (PLA(2)) were isolated by size exclusion chromatography and reversed-phase chromatography from the crude Vipera lebetina venom. The molecular masses of VLPLA(2)-1 (13,704 Da) and VLPLA(2)-2 (13,683 Da) and their internal tryptic peptides were determined by
Mariana A P Rodrigues et al.
Toxicology, 323, 109-124 (2014-06-29)
Envenoming by the pitviper Bothrops jararacussu produces cardiovascular alterations, including coagulopathy, systemic hemorrhage, hypotension, circulatory shock and renal failure. In this work, we examined the activity of this venom in rat isolated right atria. Incubation with venom (0.025, 0.05, 0.1
P S F Barbosa et al.
Toxicon : official journal of the International Society on Toxinology, 46(4), 376-386 (2005-08-24)
Bothrops jararacussu myotoxin I (BthTx-I; Lys 49) and II (BthTX-II; Asp 49) were purified by ion-exchange chromatography and reverse phase HPLC. In this work we used the isolated perfused rat kidney method to evaluate the renal effects of B. jararacussu
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