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Showing 1-30 of 54 results for "D8064" within Papers
Yin Chen et al.
Macromolecular bioscience, 15(3), 426-436 (2014-11-26)
Microgrooved thin PLGA film (≈30 μm) is successfully fabricated on a Teflon mold, which could be readily peeled off and is used for the construction of a biomimetic cardiac patch. The contraction of it is studied with optical mapping on transmembrane
Jing Wang et al.
Guang pu xue yu guang pu fen xi = Guang pu, 28(3), 617-620 (2008-06-10)
Recently, non-invasive optical methods to monitor transmembrane electrical potential using voltage sensitive dyes have been applied widely in the studies of normal and pathological heart rhythms and defibrillation. In the present paper, the authors measured the excitation and the emission
Katharine M Dibb et al.
Circulation. Heart failure, 2(5), 482-489 (2009-10-08)
In ventricular myocytes, the majority of structures that couple excitation to the systolic rise of Ca(2+) are located at the transverse tubular (t-tubule) membrane. In the failing ventricle, disorganization of t-tubules disrupts excitation contraction coupling. The t-tubule membrane is virtually
James E Reeve et al.
Biophysical journal, 103(5), 907-917 (2012-09-27)
Numerous dyes are available or under development for probing the structural and functional properties of biological membranes. Exogenous chromophores adopt a range of orientations when bound to membranes, which have a drastic effect on their biophysical behavior. Here, we present
Thomas T Luyben et al.
Frontiers in neural circuits, 14, 24-24 (2020-06-26)
cAMP is a positive regulator tightly involved in certain types of synaptic plasticity and related memory functions. However, its spatiotemporal roles at the synaptic and neural circuit levels remain elusive. Using a combination of a cAMP optogenetics approach and voltage-sensitive
Matthew E L Hardy et al.
Journal of pharmacological and toxicological methods, 60(1), 94-106 (2009-05-06)
Evaluation of drug candidates in in-vitro assays of action potential duration (APD) is one component of preclinical safety assessment. Current assays are limited by technically-demanding, time-consuming electrophysiological methods. This study aimed to assess whether a voltage-sensitive dye-based assay could be
Zaid Aqrawe et al.
PloS one, 15(8), e0237709-e0237709 (2020-08-21)
In this paper, we aim to detail the setup of a high spatio-temporal resolution, electrical recording system utilising planar microelectrode arrays with simultaneous optical imaging suitable for evaluating microelectrode performance with a proposed 'performance factor' metric. Techniques that would facilitate
Jessica M Miller et al.
Toxicology and applied pharmacology, 406, 115213-115213 (2020-09-03)
The limited availability of human heart tissue and its complex cell composition are major limiting factors for the reliable testing of drug efficacy and toxicity. Recently, we developed functional human and pig heart slice biomimetic culture systems that preserve the
Anders Peter Larsen et al.
Heart rhythm, 9(9), 1493-1500 (2012-04-28)
Voltage-sensitive dyes are important tools for mapping electrical activity in the heart. However, little is known about the effects of voltage-sensitive dyes on cardiac electrophysiology. To test the hypothesis that the voltage-sensitive dye di-4-ANEPPS modulates cardiac impulse propagation. Electrical and
Yoko Tominaga et al.
Frontiers in cellular neuroscience, 12, 389-389 (2018-11-09)
Activity-dependent changes in the input-output (I-O) relationship of a neural circuit are central in the learning and memory function of the brain. To understand circuit-wide adjustments, optical imaging techniques to probe the membrane potential at every component of neurons, such
Nikolay Aseyev et al.
Journal of neuroscience methods, 212(1), 17-27 (2012-09-18)
Optical recording of membrane potential changes with fast voltage-sensitive dyes (VSDs) in neurons is one of the very few available methods for studying the generation and propagation of electrical signals to the distant compartments of excitable cells. The more lipophilic
Zachary Laksman et al.
Scientific reports, 7(1), 5268-5268 (2017-07-15)
Since current experimental models of Atrial Fibrillation (AF) have significant limitations, we used human embryonic stem cells (hESCs) to generate an atrial-specific tissue model of AF for pharmacologic testing. We generated atrial-like cardiomyocytes (CMs) from hESCs which preferentially expressed atrial-specific
Milan Gautam et al.
Journal of molecular and cellular cardiology, 81, 139-149 (2015-03-01)
The transplantation of adipose tissue-derived stem cells (ADSCs) improves cardiac contractility after myocardial infarction (MI); however, little is known about the electrophysiological consequences of transplantation. The purpose of this study was to clarify whether the transplantation of ADSCs increases or
Stefano Casali et al.
Communications biology, 3(1), 635-635 (2020-11-01)
Long-term synaptic plasticity is thought to provide the substrate for adaptive computation in brain circuits but very little is known about its spatiotemporal organization. Here, we combined multi-spot two-photon laser microscopy in rat cerebellar slices with realistic modeling to map
Daniela Gandolfi et al.
Neural plasticity, 2015, 284986-284986 (2015-08-22)
Understanding the spatiotemporal organization of long-term synaptic plasticity in neuronal networks demands techniques capable of monitoring changes in synaptic responsiveness over extended multineuronal structures. Among these techniques, voltage-sensitive dye imaging (VSD imaging) is of particular interest due to its good
Daniela Gandolfi et al.
Frontiers in cellular neuroscience, 11, 184-184 (2017-07-14)
The induction of long-term potentiation and depression (LTP and LTD) is thought to trigger gene expression and protein synthesis, leading to consolidation of synaptic and neuronal changes. However, while LTP and LTD have been proposed to play important roles for
Carola Städele et al.
Journal of neuroscience methods, 203(1), 78-88 (2011-10-04)
Optical imaging using voltage-sensitive dyes (VSDs) is a promising technique for the simultaneous activity recording of many individual neurons. While such simultaneous recordings are critical for the understanding of the integral functionality of neural systems, functional interpretations on a single
M A Breĭgina et al.
Tsitologiia, 54(1), 85-88 (2012-05-10)
Here we give a critical analysis of the opinion of Andreev (2011) on membrane potential distribution along the pollen tube plasmalemma. He assumes that a lateral gradient of dipole potential exists, but suggests a lateral gradient of transmembrane potential impossible.
M Novakova et al.
General physiology and biophysics, 27(1), 45-54 (2008-04-26)
Voltage-sensitive dyes (VSDs) are used to record transient potential changes in various cardiac preparations. In our laboratory, action potentials have been recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining were compared
Suguru Ohira et al.
Scientific reports, 7, 42401-42401 (2017-02-12)
Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy
Giedrius Kanaporis et al.
Journal of biomedical optics, 17(9), 96007-96001 (2012-10-23)
Voltage-sensitive fluorescent dyes have become a major tool in cardiac and neuro-electrophysiology. Achieving high signal-to-noise ratios requires increased illumination intensities, which may cause photobleaching and phototoxicity. The optimal range of illumination intensities varies for different dyes and must be evaluated
Fuminori Odagiri et al.
PloS one, 9(7), e101838-e101838 (2014-07-08)
Inherited dilated cardiomyopathy (DCM) is characterized by dilatation and dysfunction of the ventricles, and often results in sudden death or heart failure (HF). Although angiotensin receptor blockers (ARBs) have been used for the treatment of HF, little is known about
Tariq Zaman et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4540-4551 (2017-03-30)
Habituation is a basic form of implicit learning and represents a sensory filter that is disrupted in autism, schizophrenia, and several other mental disorders. Despite extensive research in the past decades on habituation of startle and other escape responses, the
Toshiyuki Osakai et al.
Analytical and bioanalytical chemistry, 395(4), 1055-1061 (2009-07-10)
The spectrofluorometric behavior of a membrane potential-sensitive dye, 1-(3-sulfonatopropyl)-4-[beta-{2-(di-N-butylamino)-6-naphthyl}vinyl]pyridinium betaine (di-4-ANEPPS), at the polarized 1,2-dichloroethane/water interface was studied by means of potential-modulated fluorescence (PMF) spectroscopy. The results, combined with those from cyclic and alternating current voltammetry, clearly suggested that the
Gerard A Marchal et al.
Acta physiologica (Oxford, England), 230(2), e13493-e13493 (2020-05-10)
The voltage-gated sodium channel NaV 1.5, encoded by SCN5A, is essential for cardiac excitability and ensures proper electrical conduction. Early embryonic death has been observed in several murine models carrying homozygous Scn5amutations. We investigated when sodium current (INa ) becomes
Teresa Soda et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(13), 2383-2397 (2019-01-31)
Autism spectrum disorders (ASDs) are pervasive neurodevelopmental conditions that often involve mutations affecting synaptic mechanisms. Recently, the involvement of cerebellum in ASDs has been suggested, but the underlying functional alterations remained obscure. We investigated single-neuron and microcircuit properties in IB2
Yun Qiao et al.
Progress in biophysics and molecular biology, 144, 139-150 (2018-07-02)
Human cardiac slices have emerged as a promising model of the human heart for scientific research and drug testing. Retaining the normal tissue architecture, a multi-cell type environment, and the native extracellular matrix, human cardiac slices faithfully replicate organ-level adult
J M Davidenko et al.
Nature, 355(6358), 349-351 (1992-01-23)
Excitable media can support spiral waves rotating around an organizing centre. Spiral waves have been discovered in different types of autocatalytic chemical reactions and in biological systems. The so-called 're-entrant excitation' of myocardial cells, causing the most dangerous cardiac arrhythmias
Alexandra D Ivanova et al.
The journal of physiological sciences : JPS, 68(5), 617-628 (2017-09-21)
Rodent thoracic veins are characterized by an extended myocardial coating. In the present study, the electrical activity in the cardiac tissue of the rat azygos vein (AZV) was investigated for the first time. The atrial-like action potentials (AP) and atrial-like
Alysia Ross et al.
Molecular brain, 13(1), 108-108 (2020-08-02)
Parkinson's disease (PD) is characterized by the formation of toxic, fibrillar form alpha-synuclein (α-Syn) protein aggregates in dopaminergic neurons. Accumulating evidence has shown a multifactorial interplay between the intracellular calcium elevation and α-Syn dynamics. However, whether membrane depolarization regulates toxic
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