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Showing 1-30 of 191 results for "D8906" within Papers
Marcela Raices et al.
Developmental cell, 41(5), 540-554 (2017-06-07)
Nuclear pore complexes (NPCs) are multiprotein channels connecting the nucleus with the cytoplasm. NPCs have been shown to have tissue-specific composition, suggesting that their function can be specialized. However, the physiological roles of NPC composition changes and their impacts on
John G Facciponte et al.
The Journal of clinical investigation, 124(4), 1497-1511 (2014-03-20)
Tumor endothelial marker 1 (TEM1; also known as endosialin or CD248) is a protein found on tumor vasculature and in tumor stroma. Here, we tested whether TEM1 has potential as a therapeutic target for cancer immunotherapy by immunizing immunocompetent mice
Noa Sher et al.
Methods in molecular medicine, 122, 301-319 (2006-03-04)
Placental progesterone synthesis in humans prevents abortion of the fetus by maintaining uterine quiescence and low myometrial excitability. In rodents, a transient steroidogenic output is observed in the trophoblast giant cells during mid-pregnancy. Although the exact role of this locally
Anna Hupalowska et al.
Cell, 175(7), 1902-1916 (2018-12-15)
Nuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of the methyltransferase CARM1 during these stages results in differential methylation of histone H3R26 to
Bradley S Carter et al.
Methods (San Diego, Calif.), 52(4), 322-331 (2010-08-12)
The analysis of the spatial patterning of mRNA expression is critically important for assigning functional and physiological significance to a given gene product. Given the tens of thousands of mRNAs in the mammalian genome, a full assessment of individual gene
Violaine I Mottier-Pavie et al.
Developmental biology, 417(1), 50-62 (2016-07-02)
The mechanisms that modulate and limit the signaling output of adult stem cell niches remain poorly understood. To gain further insights into how these microenvironments are regulated in vivo, we performed a candidate gene screen designed to identify factors that
Alexandra B Rebocho et al.
eLife, 6 (2017-02-09)
Out-of-plane tissue deformations are key morphogenetic events during plant and animal development that generate 3D shapes, such as flowers or limbs. However, the mechanisms by which spatiotemporal patterns of gene expression modify cellular behaviours to generate such deformations remain to
Anna Lyubimova et al.
Nature protocols, 8(9), 1743-1758 (2013-08-21)
We present a protocol for visualizing and quantifying single mRNA molecules in mammalian (mouse and human) tissues. In the approach described here, sets of about 50 short oligonucleotides, each labeled with a single fluorophore, are hybridized to target mRNAs in
Jakob William von Trotha et al.
The European journal of neuroscience, 40(9), 3302-3315 (2014-08-26)
The brain reward circuitry plays a key role in emotional and motivational behaviors, and its dysfunction underlies neuropsychiatric disorders such as schizophrenia, depression and drug addiction. Here, we characterized the neuronal activity pattern induced by acute amphetamine administration and during
Qian Zhang et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 30(6), 479-485 (2012-06-06)
The hypothalamus is integral to the regulation of body homeostasis, including food intake, energy balance, and blood pressure. Dysfunction of the hypothalamus has been associated with a broad range of disorders; many of which are sex-dependent in prevalence. Small nucleolar
Alžběta Němečková et al.
Cytogenetic and genome research, 159(1), 48-53 (2019-10-15)
Visualizing the spatiotemporal organization of the genome will improve our understanding of how chromatin structure and function are intertwined. Here, we describe a further development of the CRISPR/Cas9-based RNA-guided endonuclease-in situ labeling (RGEN-ISL) method. RGEN-ISL allowed the differentiation between vertebrate-type
Yuhang Fan et al.
Nature methods, 20(8), 1179-1182 (2023-06-23)
Capture array-based spatial transcriptomics methods have been widely used to resolve gene expression in tissues; however, their spatial resolution is limited by the density of the array. Here we present expansion spatial transcriptomics to overcome this limitation by clearing and
Ting-Xi Yu et al.
JCI insight, 7(19) (2022-10-11)
Intestinal epithelial integrity is commonly disrupted in patients with critical disorders, but the exact underlying mechanisms are unclear. Long noncoding RNAs transcribed from ultraconserved regions (T-UCRs) control different cell functions and are involved in pathologies. Here, we investigated the role
Lindy Jensen et al.
Journal of cell science, 134(14) (2021-06-25)
Tissue-specific stem cells maintain tissue homeostasis by providing a continuous supply of differentiated cells throughout the life of organisms. Differentiated/differentiating cells can revert back to a stem cell identity via dedifferentiation to help maintain the stem cell pool beyond the
Li Zhu et al.
PLoS genetics, 17(6), e1009655-e1009655 (2021-06-29)
During spermatogenesis, the process in which sperm for fertilization are produced from germline cells, gene expression is spatiotemporally highly regulated. In Drosophila, successful expression of extremely large male fertility factor genes on Y-chromosome spanning some megabases due to their gigantic
Delphine Gogendeau et al.
Nature communications, 6, 8894-8894 (2015-11-18)
Aneuploidy is associated with a variety of diseases such as cancer and microcephaly. Although many studies have addressed the consequences of a non-euploid genome in cells, little is known about their overall consequences in tissue and organism development. Here we
Regulation of intestinal stem cell activity by a mitotic cell cycle regulator Polo in Drosophila
Zhang et al.
G3 (Bethesda, Md.), 16, 84-84 (2023)
Miao Liu et al.
Nature communications, 11(1), 2907-2907 (2020-06-11)
The three-dimensional architecture of the genome affects genomic functions. Multiple genome architectures at different length scales, including chromatin loops, domains, compartments, and lamina- and nucleolus-associated regions, have been discovered. However, how these structures are arranged in the same cell and
Joost F Swennenhuis et al.
Nucleic acids research, 40(3), e20-e20 (2011-11-30)
FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic
Four-Color STED Super-Resolution RNA Fluorescent In Situ Hybridization and Immunocytochemistry to Visualize Mitochondrial mRNAs in Context with Mitochondrial Ribosomes.
Albus, et al.
Methods in Molecular Biology, 2661, 303-316 (2023)
Sheel Shah et al.
Cell, 174(2), 363-376 (2018-06-12)
Visualization of the transcriptome and the nuclear organization in situ has been challenging for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method, intron seqFISH, that allows imaging of 10,421 genes at their nascent transcription active sites in
Stephan M Schulreich et al.
Organisms, diversity & evolution, 22(4), 893-913 (2022-11-19)
The mesoderm gives rise to several key morphological features of bilaterian animals including endoskeletal elements and the musculature. A number of regulatory genes involved in mesoderm and/or muscle formation (e.g., Brachyury (Bra), even-skipped (eve), Mox, myosin II heavy chain (mhc))
Aakash Koppula et al.
Cancers, 14(2) (2022-01-22)
Circular RNAs (circRNAs) are regulatory RNAs which have recently been shown to have clinical significance in several diseases, including, but not limited to, various cancers, neurological diseases and cardiovascular diseases. The function of such regulatory RNAs is largely dependent on
A Pauciullo et al.
Theriogenology, 78(3), 688-695 (2012-04-17)
The present study reports on the frequency of X-Y aneuploidy in the sperm population of two minor cattle breeds reared in Italy, namely Modicana and Agerolese, which are listed in the "Anagraphic Register of autochthonous cattle populations with limited distribution".
Maria V Sharakhova et al.
Methods in molecular biology (Clifton, N.J.), 1858, 177-194 (2018-11-11)
The development of genomic resources and tools is an important step in designing novel approaches to genetic control of mosquitoes. Physical genome maps enhance the quality of the genome assemblies, improve gene annotation, and provide a better framework for comparative
Ezgi Hacisuleyman et al.
Nature structural & molecular biology, 21(2), 198-206 (2014-01-28)
RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we
Analysis of transgenic mice.
Stefan Selbert et al.
Methods in molecular biology (Clifton, N.J.), 180, 305-341 (2002-03-05)
Nayuta Yakushiji-Kaminatsui et al.
Methods in molecular biology (Clifton, N.J.), 2529, 207-228 (2022-06-23)
Among the most important histone methyltransferases for metazoan development are EZH1/2 and their homologs, which methylate histone H3 lysine 27 and act as part of a highly conserved set of chromatin regulators called Polycomb Group (PcG) proteins. Reaching a precise
Fuxiang Wei et al.
Nature communications, 11(1), 4902-4902 (2020-10-01)
Living cells and tissues experience various complex modes of forces that are important in physiology and disease. However, how different force modes impact gene expression is elusive. Here we apply local forces of different modes via a magnetic bead bound
Mona Batish et al.
Methods in molecular biology (Clifton, N.J.), 714, 3-13 (2011-03-25)
This protocol describes a method to image individual mRNA molecules in situ. About 50 oligonucleotides complementary to different regions of a target mRNA species are used simultaneously. Each probe is labeled with a single fluorescent moiety. When these probes bind
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