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Showing 1-30 of 38 results for "E6511" within Papers
Alexandre Prola et al.
Bio-protocol, 11(20), e4201-e4201 (2021-11-12)
The efficient ATP production in mitochondria relies on the highly specific organization of its double membrane. Notably, the inner mitochondrial membrane (IMM) displays a massive surface extension through its folding into cristae, along which concentrate respiratory complexes and oligomers of
Germán Belenguer et al.
STAR protocols, 2(2), 100425-100425 (2021-04-27)
This protocol provides a flow-cytometry-based procedure to classify and isolate all cells of the adult rodent subependymal zone (SEZ) neurogenic lineage, without the need for reporter mice, into different cell populations, including three neural stem cell (NSC) fractions with molecular
Modulation of matrix metalloproteinase activity by EDTA prevents posterior capsular opacification
Hazra S, et al.
Molecular Vision, 18(6), 1701-1701 (2012)
José González-Martínez et al.
JCI insight, 6(16) (2021-07-09)
Congenital microcephaly (MCPH) is a neurodevelopmental disease associated with mutations in genes encoding proteins involved in centrosomal and chromosomal dynamics during mitosis. Detailed MCPH pathogenesis at the cellular level is still elusive, given the diversity of MCPH genes and lack
Juan Tornín et al.
Free radical biology & medicine, 164, 107-118 (2021-01-06)
The use of oxidative stress generated by Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of
José González-Martínez et al.
Cell death and differentiation, 29(8), 1474-1485 (2022-01-22)
Alteration of centrosome function and dynamics results in major defects during chromosome segregation and is associated with primary autosomal microcephaly (MCPH). Despite the knowledge accumulated in the last few years, why some centrosomal defects specifically affect neural progenitors is not
Peri H Tate et al.
Methods (San Diego, Calif.), 53(4), 331-338 (2011-02-04)
The EUCOMM and KOMP programs have generated targeted conditional alleles in mouse embryonic stem cells for nearly 10,000 genes. The availability of these stem cell resources will greatly accelerate the functional analysis of genes in mice and in cultured cells.
Elisa Cuevas et al.
Frontiers in cell and developmental biology, 3, 20-20 (2015-04-18)
Lin41/Trim71 is a heterochronic gene encoding a member of the Trim-NHL protein family, and is the original, genetically defined target of the microRNA let-7 in C. elegans. Both the LIN41 protein and multiple regulatory microRNA binding sites in the 3'
Pascal Röderer et al.
Pain, 164(8), 1718-1733 (2023-02-03)
Induced pluripotent stem cells (iPSCs) have enabled the generation of various difficult-to-access cell types such as human nociceptors. A key challenge associated with human iPSC-derived nociceptors (hiPSCdNs) is their prolonged functional maturation. While numerous studies have addressed the expression of
Sergiu Cecoltan et al.
Frontiers in cardiovascular medicine, 8, 714573-714573 (2021-08-31)
Background: Valvular endothelial cells (VEC) have key roles in maintaining valvular integrity and homeostasis, and dysfunctional VEC are the initiators and major contributors to aortic valve disease in diabetes. Previous studies have shown that HG stimulated an inflammatory phenotype in
Accurate Results in the Clinical Laboratory: A Guide to Error Detection and Correction (2013)
Takamichi Miyazaki et al.
Current protocols in stem cell biology, 32, 1C-1C (2015-02-03)
This unit describes a protocol for efficient expansion of human pluripotent stem cells (hPSCs). A key feature of this method is subculture of hPSCs by single-cell dissociation passaging on substrates coated with recombinant E8 fragments of human laminin isoforms (LM-E8s).
Sylwia Mazurek et al.
Cells, 10(8) (2021-08-28)
TRIM28, a multi-domain protein, is crucial in the development of mouse embryos and the maintenance of embryonic stem cells' (ESC) self-renewal potential. As the epigenetic factor modulating chromatin structure, TRIM28 regulates the expression of numerous genes and is associated with
Judit E Šponer et al.
iScience, 26(9), 107600-107600 (2023-09-04)
Accumulation and selection of nucleotides is one of the most challenging problems surrounding the origin of the first RNA molecules on our planet. In the current work we propose that guanosine 3',5' cyclic monophosphate could selectively crystallize upon evaporation of
Ana Domingo-Muelas et al.
Nature communications, 14(1), 373-373 (2023-01-24)
Neural stem cells (NSCs) in the adult murine subependymal zone balance their self-renewal capacity and glial identity with the potential to generate neurons during the lifetime. Adult NSCs exhibit lineage priming via pro-neurogenic fate determinants. However, the protein levels of
Alan M Tartakoff et al.
Current biology : CB, 31(12), 2507-2519 (2021-04-17)
In ribosomal DNA (rDNA) repeats, sequences encoding small-subunit (SSU) rRNA precede those encoding large-subunit (LSU) rRNAs. Processing the composite transcript and subunit assembly requires >100 subunit-specific nucleolar assembly factors (AFs). To investigate the functional organization of the nucleolus, we localized
Gitte Lund et al.
International archives of allergy and immunology, 153(4), 323-334 (2010-06-19)
Allergen-specific immunotherapy (SIT) leads to reduced symptoms upon allergen exposure through as yet unresolved mechanisms. Desensitization of basophils to specific allergens during the updosing phase of injection immunotherapy may contribute to the clinical effect of SIT. Here we report a
Lou Lambert et al.
Bio-protocol, 14(15), e5043-e5043 (2024-08-12)
Chlamydomonas (Chlamydomonas reinhardtii) is a unicellular model alga that has been shown to undergo programmed cell death (PCD) that can be triggered in response to different stresses. We have recently shown that Chlamydomonas is particularly well suited to the study
Krishnendu Khan et al.
STAR protocols, 3(1), 101201-101201 (2022-03-15)
Despite recent advances in structural determination of individual proteins, elucidating the 3-dimensional architecture of large, multiprotein complexes remains challenging, partly because of issues related to structural integrity during purification. Here, we describe a protocol to determine the 3-dimensional architecture of
What is the best protocol to cryopreserve immature mouse testicular cell suspensions?
Onofre J, et al.
Reproductive Biomedicine Online, 37(1), 6-17 (2018)
Phillippa J Carling et al.
Autophagy, 19(2), 692-705 (2022-07-06)
The accumulation of toxic protein aggregates in multiple neurodegenerative diseases is associated with defects in the macroautophagy/autophagy-lysosome pathway. The amelioration of disease phenotypes across multiple models of neurodegeneration can be achieved through modulating the master regulator of lysosome function, TFEB
Ainara González-Iglesias et al.
Nature communications, 15(1), 2837-2837 (2024-04-03)
The adult mammalian brain retains some capacity to replenish neurons and glia, holding promise for brain regeneration. Thus, understanding the mechanisms controlling adult neural stem cell (NSC) differentiation is crucial. Paradoxically, adult NSCs in the subependymal zone transcribe genes associated
Ashok Kumar et al.
Nature protocols, 5(11), 1737-1747 (2010-10-30)
In cell affinity chromatography, type-specific cell separation is based on the interaction between cell-surface receptors and an immobilized ligand on a stationary matrix. This protocol describes the preparation of monolithic polyacrylamide and polydimethylacrylamide cryogel affinity matrices that can be used
Ivonne Maciel Arciniega-Martínez et al.
Molecular medicine reports, 25(2) (2021-12-28)
Life stress may influence symptom onset and severity in certain gastrointestinal disorders in association with a dysregulated intestinal barrier. It has been widely accepted that stress triggers the hypothalamus‑pituitary‑adrenal (HPA) axis, releasing corticosterone, which promotes intestinal permeability. In response, colonic
Sedat Odabas et al.
Methods in molecular biology (Clifton, N.J.), 1109, 47-63 (2014-01-30)
Mesenchymal stem cells (MSCs) have drawn great interest in the field of regenerative medicine, for cell replacement, immunomodulatory, and gene therapies. It has been shown that these multipotent stromal cells can be isolated from tissues such as bone marrow, adipose
Vladimir P Baklaushev et al.
Drug delivery, 22(3), 276-285 (2014-01-21)
Targeted drug delivery for brain tumor treatment is one of the important objectives in nanomedicine. Human glioblastoma is the most frequent and aggressive type of brain tumors. The preferential expression of membrane protein connexin 43 (Cx43) and brain-specific anion transporter
Tomas Zelenka et al.
Methods and protocols, 4(3) (2021-07-22)
The functional implications of the three-dimensional genome organization are becoming increasingly recognized. The Hi-C and HiChIP research approaches belong among the most popular choices for probing long-range chromatin interactions. A few methodical protocols have been published so far, yet their
Kamila Kulig et al.
International journal of molecular sciences, 24(24) (2023-12-23)
Human immune cells possess the ability to react complexly and effectively after contact with microbial virulence factors, including those transported in cell-derived structures of nanometer sizes termed extracellular vesicles (EVs). EVs are produced by organisms of all kingdoms, including fungi
Tom Verbeelen et al.
iScience, 27(5), 109596-109596 (2024-04-19)
The advancement of regenerative life support systems (RLSS) is crucial to allow long-distance space travel. Within the Micro-Ecological Life Support System Alternative (MELiSSA), efficient nitrogen recovery from urine and other waste streams is vital to produce liquid fertilizer to feed
Erica Butti et al.
Nature communications, 13(1), 7579-7579 (2022-12-10)
The adult brain retains over life endogenous neural stem/precursor cells (eNPCs) within the subventricular zone (SVZ). Whether or not these cells exert physiological functions is still unclear. In the present work, we provide evidence that SVZ-eNPCs tune structural, electrophysiological, and
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