3D cell culture
Applied Filters:
Applications:3D cell culture
Content Type:Protocol
Showing 1-21 of 21 results
TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol
TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.
Cancer Stem Cell Tumorsphere Formation Protocol
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Cultrex® 96-Well 3D Spheroid BME Cell Invasion Assay Protocol
The 96 Well 3D Spheroid BME Cell Invasion Assay utilizes 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs
Cultrex ® 3-D Culture Matrix™ Laminin I Protocol
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel
An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation
Poly-L-Lysine Cell Attachment Protocol
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Tumor Spheroid Formation Assay
3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.
Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration
Learn the results that come from changing the protein concentration of Corning® Matrigel®.
In Vitro T Cell Killing Assay Protocol Using Millicell® Microwell Plates
Learn how to set up a T cell killing assay using Millicell® Microwell plates. Our high throughput assay protocol evaluates the killing capacity to colorectal tumor spheroids and enables patient-specific assays.
TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol
A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available
Corning® Spheroid Microplates
Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well spheroid microplate format.
Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol
3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.
Protocol Guide: Expansion and Differentiation of Human Mesenchymal Stem Cells using TrueGel3D® HTS Hydrogel Plates
Learn how to culture human adipose mesenchymal stem cells (MSCs) in 3D hydrogels like the TrueGel3D® Hydrogel Plate using this step-by-step protocol for high-throughput screening cell culture applications.
Laminin Coating Protocol for Cell Culture
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol
TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits
Protocol Guide: Differentiation of Human Neural Stem Cells using TrueGel3D® HTS Hydrogel Plates
Learn to culture ReNcell® human neural stem cells (NSCs) in 3D hydrogels like the TrueGel3D® HTS Hydrogel Plate using this step by step protocol for high-throughput screening cell culture applications.
Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro
Learn how the Corning® Matrigel® Basement Membrane Matrix can be used to boost your 3D Cell Culture activities.
Organoids Generation and Phenotypic Screen on Millicell® Microwell Plates
Discover how to use Millicell® Microwell plates to generate mouse intestinal and colorectal cancer organoids and develop a high throughput phenotypic drug development screen.