3D cell culture
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An ECM Mimetic Library for Engineering Surfaces to Direct Cell Surface Receptor Binding Specificity and Signaling
The extracellular (ECM) microenvironment, defined by biochemical cues and physical cues, is a deciding factor in a wide range of cellular processes including cell adhesion, proliferation, differentiation, and expression of phenotype-specific functions. For this reason, engineering the ECM microenvironment provides
True Gel3D™ Hydrogel Troubleshooting Guide
TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide
dECM hydrogels 3D culture
Comparison of decellularized extracellular matrix (dECM) hydrogels to Matrigel® matrices for predictive 3D cell culture models
High-Throughput Drug Screening using TrueGel3D® HTS Hydrogel Plates
Compound cytotoxicity screening assays with 3D cell cultures using TrueGel3D® HTS Hydrogel Plates including dose response curves, IC50 determination, and spheroid killing assay data.
What is the Extracellular Matrix?
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Hydrogels for 3D Cell Culture
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
TrueGel3D™ Hydrogel for 3D Cell Culture
TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
Utilization of TrueGel3D™ Hydrogels to Study Fibroblast Spreading
Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and
Cyst Formation of MDCK Cells in TrueGel3D™ Hydrogels
TrueGel3D synthetic hydrogel can be used to study cyst formation of MDCK cells.
3D Imaging of Optically Cleared Spheroids in Corning® Spheroid Microplates
Discover how Corning® Spheroid Microplates played a key role in the use of use of 3D cell cultures for in vitro drug discovery assay.
3D Cell Culture with Corning Microplates
Take a deep dive into the world of 3D cell culture courtesy of Corning and understand why the Corning® spheroid microplate can be an asset in the evolution of 3D cell culture.
Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
High-Throughput 3D Cell Culture Using TrueGel3D® HTS Hydrogel Plates
The TrueGel3D® HTS Hydrogel Plate is a ready-to-use solution to easily establish 3D cell cultures using fully synthetic hydrogels in a simple and automation-compatible manner.
Co-culture of Tumor and Stromal Cells in TrueGel3D™ Hydrogels
TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.
Lung Cancer Organoid Biobank
Explore our cryopreserved human lung organoid biobank from NSCLC tumor biopsies for lung cancer, SARS-CoV2, COPD and pulmonary disease research. Lung organoid 3D cell culture protocol available.
Millicell® Microwell Plates Frequently Asked Questions (FAQs)
Explore answers to your frequency asked questions about Millicell® Microwell plates. Our plates support reliable organoid culture with array of ultra-dense microwells in hydrogel for immunology, drug development, and cell therapy applications.
Millicell® Ultra-Low Attachment Plates Spheroid Formation Performance Comparison
Learn about the similarities and differences in spheroid formation, roundness, and circularity between Millicell® ultra-low attachment plates and other ULA plates.
Evaluation of Spheroid Formation and Robustness using Millicell® Ultra-low Attachment Plates
Learn how to quantify spheroid formation, roundness, and circularity. Discover Millicell® Ultra-low Attachment plates, an ideal 3D cell culture model for uniform spheroid formation.
HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications
Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.
Cell Migration and Invasion Assay Guidance using Millicell® Cell Culture Inserts
How to set up cell migration assays using cell culture inserts.
What is Hyaluronan?
Hyaluronan is a major component of the extracellular matrix. It’s the simplest glycosaminoglycan, and it plays several biological roles.
Tissue-Specific Decellularized Extracellular Matrix (dECM) Hydrogels for Enhanced 3D Cell Cultures
To isolate the native extracellular matrix of a tissue from its inhabiting cells, a decellularization process is employed.
Three-Dimensional Printing of Tissue Engineering Scaffolds
We will explore the technological advances that have contributed toward the progress of 3DP of tissue engineering scaffolds, current materials used to create 3DP scaffolds, and the challenges that remain.
Attachment Factors for Three-dimensional Cell Culture
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
CytoSoft® Elastic Modulus Plates for Cell Culture
CytoSoft® elastic modulus plates are cell culture tools used to accurately analyze the effect of substrate stiffness/rigidity on cell behavior for mechanobiology applications covering a broad physiological range of 0.2 kPA- 64 kPa.
ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Create a 3D kidney nephrotoxicity model on the AIM Biotech 3D cell culture chip
AIM Biotech’s easy-to-use modular platform for conducting 3D cell culture into your research. The AIM Biotech organ-on-a-chip enables you to run simple assays or create more complex microphysiological systems. The versatile platform is suitable for a wide variety of cell
What is a Cell Delivery Vehicle?
Cell delivery vehicle are components that can be associated with cells and that allow transplantation in human or animals hosts. These can be natural or synthetic components that will form a gel, embedding the cells.
Fibronectin
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media
Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.
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