DNA and RNA purification
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Applications:DNA and RNA purification
Content Type:Technical Article
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KAPA Express Extract Kit FAQs
Frequently asked questions (FAQs) for KAPA Express Extract Kit.
Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles
Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.
Oligonucleotide Purification
Discover the best purification option for your oligo, based on the intended application.
General Considerations for Plasmid DNA Preparation
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
DNA and RNA Quantification
An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Genomic DNA Sample Purification and Quality Assessment
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of
RNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during RNA preparation.
RNA Isolation from Blood, Cultured Cells, and Animal or Plant Tissue on Whatman™ FTA™ Cards
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Plasmid DNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
Lentiviral Packaging
The goal of this exercise is to learn lentiviral packaging. For this example we will be using the HEK 293T cell line.
ECACC Nucleic Acids
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
High Pure Technology and Silica Adsorption Kits
The High Pure and silica adsorption kits developed by Roche depend on the tendency of nucleic acids to adsorb to silica (glass) in the presence of a chaotropic salt such as sodium iodide (NaI), guanidine thiocyanate or guanidine hydrochloride [Melzak
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that
How to Determine the Best Product & Supplier for Proteinase K
The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase
An Introduction to Proteinase K and Its Applications
Proteinase K is commonly used in molecular biology and biochemistry applications to digest structural proteins and enzymes. It is useful in removing nucleases that can degrade DNA and RNA, as well as in the isolation of intact genomic DNA from
MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows | Sigma-Aldrich
Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes
Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome
Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA
Genomic DNA Preparation Troubleshooting
Description of common problems that can occur during genomic DNA preparation
Downstream Applications after Genomic DNA Purification
This page shows downstream applications after genomic DNA purification.
Protein Affinity Chromatography
Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires
Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit
This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.
Biomatrica® offers an innovative alternative to reliance on cold chain logistics
Biomatrica® products enable researchers to stabilize, ship, and store biological samples for long periods of time.
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
Sodium acetate buffer solution for molecular biology
Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .
Stabilizing RNA at room temperature in RNAstable®
RNAstable® is a novel preservation product developed to protect
RNA from degradation during storage or shipment at ambient
temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by some organisms
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