FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective
Experimentally determine the appropriate antibiotic concentration for selecting stable cell lines. This titration protocol can determine the lowest concentration of puromycin needed to efficiently select transduced cells.
Upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest (this varies, but is typically 5 clones), we guarantee that at least one of those clones for a gene should yield greater
By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.
You are not alone designing successful CRISPR, RNAi, and ORF experiments. We were the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field
Complete protocol for Lentiviral transduction of suspension cells. This protocol describes the use of MISSION TRC shRNA Lentiviral Particles for long term silencing and phenotypic observation of suspension cells.