Gene expression and silencing

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Applications:Gene expression and silencing

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A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Experimental Design and Analysis — Experimental Design FAQs

We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.

MISSION® FAQs

We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.

General Adherent Cells

Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

TRC Design and pLKO.1 Vector FAQs

The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.

miRNA (microRNA) Introduction

Mature microRNAs (miRNAs) are a class of naturally occurring, small non-coding RNA molecules, about 21-25 nucleotides in length. MicroRNAs are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to downregulate gene expression

MISSION shRNA Lentiviral Transduction

MISSION® shRNA Lentiviral Transduction application data

Lentiviral Transduction

In this exercise we will be demonstrating lentiviral transduction on two different cell lines.

T7 RNA Polymerase - recombinant, expressed in E. coli

T7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research.

MISSION® Application Data

MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.

Custom Services

We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.

Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

MISSION^® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

esiRNA Knockdown Efficiency Tested by Western Blotting

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Delivery of Nucleic Acids Using Polymers

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

General shRNA FAQs

We answers your frequently asked questions about shRNA, the TRC. MISSION and more.

Getting Started with MISSION^® shRNA

Gene silencing and knockdown using RNA interference is becoming routine. The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

Oligonucleotide Quality Control & Quality Assurance

The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.

esiRNA FAQs (Frequently Asked Questions)

esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.

CRISPR/Cas-GFP Vectors for Rapid Expression Verification and Enrichment of Genome Edited Cells

View experimental data showing crispr/cas expression and enrichment using FACS

siRNA FAQ

We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.

MISSION® siRNA FAQs

We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.

Antisense Oligonucleotides

This article summarizes several of the common mechanisms of antisense gene modulation and more importantly, considerations to take into account when designing an antisense oligonucleotide (ASO).

Phosphorothioate Oligonucleotides

Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and

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