This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method
Under applied conditions, system suitability criteria are met, and the Chloroquine Phosphate HPLC Assay and Impurity Profiling Methods demonstrate good resolution/selectivity, reproducibility, and sensitivity.
Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Bispecific antibodies are a new class of biotherapeutics where one therapeutic agent can target two different antigens. This application note demonstrates a new size exclusion chromatographic method for characterizing these new biotherapeutics by mass spectrometry.
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.