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Lopinavir European Pharmacopeia HPLC Assay Method
This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method
HPLC Analysis of LMW Heparin-Dalteparin and Enoxaparin Analysis on Zenix®-100
High resolution separations of low molecular weight heparin-daletparin and enoxaparin can be achived using Zenix SEC-100 columns.
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Chloroquine Phosphate HPLC Assay and Impurity Profiling Methods Following the USP Monograph
Under applied conditions, system suitability criteria are met, and the Chloroquine Phosphate HPLC Assay and Impurity Profiling Methods demonstrate good resolution/selectivity, reproducibility, and sensitivity.
Faster Protein and Peptide Liquid Chromatography (FP2LC)
Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of Purified Adeno-Associated Virus (AAV) on TSKgel® SP-NPR
HPLC Analysis of Purified Adeno-associated Virus (AAV) on TSKgel® SP-NPR
Size Exclusion Analysis of Tween-Containing IgG Formulations
In this study, we investigated the effect of adding Tween® 20 and Tween 80 on the elution of proprietary mAb 221 under size exclusion chromatography conditions.
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Chromatographic Testing of Black Pepper According to the United States Pharmacopeia
Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.
Performing a Separation with Sephacryl®
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan
Analysis of a Bispecific Monoclonal Antibody Using Size Exclusion Chromatography and Mass Spectrometry
Bispecific antibodies are a new class of biotherapeutics where one therapeutic agent can target two different antigens. This application note demonstrates a new size exclusion chromatographic method for characterizing these new biotherapeutics by mass spectrometry.
Empowering Biochemists to Analyze Glycoproteins with Exceptional Reproducibility
Fast, high-resolution separations of procainamide labeled glycans - dextran ladder, human IgG and cetuximab
Installation & Use of Milli-Q® SQ 2Series Water Purification Systems
View instructional videos and download User Manuals for Milli-Q® SQ 2Series lab water systems. You’ll be up and running in no time. Buy online systems, accessories, cartridges and services.
HPLC Analysis of Glycans
HPLC Analysis of Glycans
HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
HPLC Analysis of PEG g006200
HPLC Analysis of PEG on Two Zenix® SEC-300 Columns in Tandem
Syringe Care
Syringes are precision instruments and need care. Learn to correctly inspect, use, unclog, handle, clean and store them to ensure good accuracy, reproducibility, and lifetime.
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
Performing a Separation with Sephadex®
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Workflow for High-Throughput Glycoprofiling of Rituximab
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters
Biomarkers play an essential role in the drug discovery and development process.