Low pressure liquid chromatography
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Applications:Low pressure liquid chromatography
Content Type:Protocol
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Packing a Column and Performing a Separation with Sephadex® LH-20
This page shows how to perform a separation with a Sephadex LH-20 column from Cytiva which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.
Performing a Separation with Superose
Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Removal of Detergents from Biological Product Matrices
The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.
Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Reverse Phased Chromatography (RPC) in Practice
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum
Performing a Purification with Calmodulin Sepharose 4B
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.
Chromatofocusing: Principles and Methods
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Performing a Separation with Superdex
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation for Size Exclusion Chromatography
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Capto Adhere ImpRes
Capto adhere ImpRes is a cost-effective and flexible chromatography medium from Cytiva, designed for high-resolution polishing of MAbs.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.
Capto Core 700
Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.
Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B Columns
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance
How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from Cytiva.
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