Mammalian cell culture
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Applications:Mammalian cell culture
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ECM Coating Protocols for Millicell®
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
Powdered Media Preparation Instructions
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Testing Cells for Bacterial and Fungal Contamination
Cell culture protocol for testing cell lines for bacterial and fungal contamination.
Gelatin Coating Protocol for Cell Culture
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com
Coating Tissue Culture Plates with Vitronectin
Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.
Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)
Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.
Subculture of Adherent Cell Lines
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.
StableCell™ Trypsin Solutions
StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.
Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs
Poly-L-Lysine Cell Attachment Protocol
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
Trypsin Inhibitors
Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors. Serpins control the activation and catabolism of proteins by the inhibition of serine proteases in vivo.
HL-1 Cells
We’ve got everything else you’ll need to get started in your research of your HL-1 cells. From culture plates and cryovials to the proprietary Claycomb Media, look no further for a complete offering of products and services to support your
Rat Dermal Fibroblasts (RDF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Cryopreservation of Cell Lines
Cell culture protocol for freezing cell lines at high cell viabilities using cryopreservation reagents such as DMSO. Cryopreservation is a method whereby cells are frozen, maintaining their viability, until they are defrosted months or years later. Free ECACC handbook download.
Cell Culture Protocol 1: Proper Aseptic Technique and Sterile Handling of Cells
Cell culture protocol for proper aseptic technique to maintain a sterile environment and to prevent cell culture contamination. Free ECACC handbook download.
Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines
Cell culture protocol for passaging and splitting semi-adherent cell lines using trypsin EDTA.
Enzymatic Glutamate Assay
Assay protocol for the colorimetric detection of Glutamate in biological samples using the Glutamate assay kit.
Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Dextran
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three
Laminin Coating Protocol for Cell Culture
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Trypsin Cell Dissociation Protocol
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.