Mammalian cell culture
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Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it
Cell Counting Using a Hemocytometer
Cell counting protocol using a hemocytometer and trypan blue for measuring cell viability.
Transferrin in Cell Culture
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
Linolenic Acid in Cell Culture
Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Identification of Cell Culture Media with Raman Spectroscopy
Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.
Common Cell Culture Problems: Precipitates
Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter
Cell Culture Protocol 5: Subculture of Suspension Cell Lines
Cell culture protocol for passaging and splitting suspension cell lines
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads
Counting of Cells
Trypan blue method of cell counting is inexpensive and readily available method. The method is based on principle that living cells exclude trypan blue dye and appear bright, while dead cells take up dye and appear blue. Watch video to
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Cyclodextrins
Many metabolically important compounds, such as lipid-soluble vitamins and hormones, have very low solubilities in aqueous solutions. Various techniques have been used to solubilize these compounds in tissue culture, cell culture, or other water-based applications.
Zinc in Cell Culture
Zinc is an essential element in cell culture. However, classical media do not typically contain it in their basal formulations. This is because sera used at 5 to 10% provides sufficient zinc for cell growth and survival.
Vascular Endothelial Growth Factors (VEGF)
Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Linoleic acid in Cell Culture
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Fibroblast Growth Factor Family (FGF)
Fibroblast growth factors in cell culture and various growth factors for your research
Aerobic Glycolysis and the Warburg Effect
We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer
Citrate Serum-Free Medium Supplement
Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.
Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.
Cell Growth to Confluence
Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.
Human Mesenchymal Stem Cells and Growth/Differentiation Media
Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and
Nutrient Mixture F-10 Ham Formulation
Nutrient Mixture F-10 Ham Formulation
Stearic Acid in Cell Culture
Fatty acids of the n-3, n-6 and n-9 families are important supplements for cell culture systems. They are important in cell culture systems used to biomanufacture heterologous proteins, such as monoclonal antibodies.
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
Cell Freezing and Banking
Cell Freezing and Banking
Lipoic (Thioctic) Acid, in Cell Culture
How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Cell Analysis Best Practices
How do you assess the state of your cell culture? We’ve got some best practices to share.
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