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L-Ornithine in Cell Culture
Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.
L-Tyrosine in Cell Culture
Learn more about the amino acid L-Tyrosine and explore how it is used in cell culture media like MEM, DMEM, and RPMI. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.
Corning® High Content Screening Microplates – Frequently Asked Questions
A frequently asked questions guide for users of the Corning® high content screening microplates.
Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
Antifungals Reagents in Cell Culture
Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.
Ascorbate in Cell Culture
Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Calcium in Cell Culture
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Cell Analysis Best Practices
How do you assess the state of your cell culture? We’ve got some best practices to share.
Cell Clumping Troubleshooting
Cell clumping in culture media results from free DNA and cell debris, affecting cell growth.
Identification of Cell Culture Media with Raman Spectroscopy
Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.
DMEM/nutrient Mixture F-12 Formulation
Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation
Evolution of Cell Culture Surfaces
For good cell attachment the hydrophobic polystyrene surface must be modified to a more hydrophilic surface. This allows cell attachment proteins (vitronectin and fibronectin) found in the serum containing culture medium to adhere and spread on the vessel bottom providing
Nutrient Mixture F-10 Ham Formulation
Nutrient Mixture F-10 Ham Formulation
F-12 Coon's Modification Formulation
F-12 Coon's Modification Formulation
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads facilitate Percoll gradient monitoring in biological separations.
L-Glutamine in Cell Culture
Importance and uses of glutamine in hybridoma and mammalian cell culture
Glutathione in Cell Culture
Learn how glutathione and other components affect serum-free cell culture systems for protein biomanufacturing.
Good Cell Banking Practices
Overview of good cell banking practices for cell line cryopreservation purposes.
Growth Factors and Cytokines
High-quality growth factors and cytokines tested for bioactivity and low endotoxin levels for optimal cell culture.
Hematopoietic Cytokines
Read article on hematopoietic cytokines and hematopoiesis
In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Hybridoma Fusion Partners Cell Lines
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
Linoleic acid in Cell Culture
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
T Cell Expansion and Viability Using Stericup®-filtered Media
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Antibiotic Kill Curve
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Cell Growth to Confluence
Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.
Citrate Serum-Free Medium Supplement
Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.
Copper in Cell Culture
Importance and uses of copper in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO), cell cultures
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