In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.