Nucleic acid labeling and detection

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Applications:Nucleic acid labeling and detection

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There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

DIG Northern Starter Kit Protocol & Troubleshooting

In general, it is possible to use DNA probes for northern blots (PCR or random-primed DNA probes). It is however important to keep in mind that DNA probes are best detecting abundant messages, such as housekeeping genes.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

DIG Easy Hyb™ Granules Protocol & Troubleshooting

Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

Oligonucleotide Melting Temperature

Learn about methods for calculating oligonucleotide melting temperature (Tm).

NBT/BCIP Stock Solution Protocol & Troubleshooting

NBT Protocol

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

DIG RNA Labeling Mix Protocol Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).