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Chemically Defined Media Supplements Improve Quality and Lower Risk in Upstream Bioprocessing
Chemically defined media supplements offer many benefits including decreased lot-to-lot variation, decreased risks, and improved glycosylation profiles.
One-step Feed Optimization for Upstream Cell Culture Processes
Optimizing feed for upstream cell culture improves monoclonal antibody production, but requires balancing outcomes for both viable cell density and productivity.
Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing
Understand how considerations such as DMSO concentrations and freezing techniques are key in the successful implementation of high cell density cryopreservation (HCDC).
Improving API Solubility
This article describes the solubility challenge presented by many small molecule APIs and strategies to overcome these issues during the formulation process.
What is an Antibody-Drug Conjugate?
ADCs are a fast-growing drug modality used as a cancer therapy. Learn more about the parts of an ADC, how bioconjugates work, and the ADC manufacturing process.
Simplifying Antibody-Drug Conjugate Payload Synthesis Using Payload Intermediates
Meta description: ADC payload intermediates such as, DOLCore ™, MAYCore ™, and PBDCore ™, simplify payload synthesis for the most commonly used payloads: maytansine, dolastatin, and PBD dimers.
Seamless Integration of Glucose Control in CHO Culture Using Raman Spectroscopy
This page describes the use of a ProCellics™ Raman Analyzer with Bio4C® PAT Raman Software to implement a feedback control loop in a CHO cell culture process to monitor glucose concentration and enable maintenance of stable glucose levels without the
Tangential Flow Filtration in the Antibody Drug Conjugate (ADC) Manufacturing Process
Find best practices for using Pellicon® Capsules in ADC processing as well as data from two studies using Pellicon® Capsules and Pellicon® cassettes in the ADC manufacturing process.
Ultrafiltration/Diafiltration (UF/DF) in Plasmid DNA Manufacturing
How are ultrafiltration and diafiltration used in plasmid DNA manufacturing and how should you optimize this process? Learn more in our technical article and download our free eBook.
API Solubility and Dissolution Enhancement Via Formulation
Our overview of API solubility and dissolution enhancement methods can help you decide between the many options to take during formulation.
Chromatographic Purification of Plasmid DNA (pDNA) for mRNA, Plasmid-based DNA Vaccines, and Viral Vector Applications
See case study examples of how to optimize chromatographic purification of plasmid DNA for Biopharmaceutical Applications.
An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts
An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.
Seed Train Intensification Using High Cell Density Cryopreservation
High cell density cryopreservation (HCDC) is a closed processing alternative to the traditional seed train expansion process. Learn more about the benefits of HCDC.
Upstream Bioprocessing Feed Strategies and Scalability: A Case Study with Cellvento® ModiFeed Prime COMP
This case study walks through optimizing feed and media for upstream bioprocessing and how it translates to large-scale bioreactors.
Optimizing Upstream Media and Feeds in Bioprocessing
Why optimize your upstream bioprocess media and feed? This article discusses the why and how of optimizing your media and feed to maximize cell viability and productivity.
Active Pharmaceutical Ingredient (API) Stability in Solid Dose Formulation
This article describes the use of excipients to help address API stability issues.
Protein Binding Determination Using Supel™ BioSPME C18 Pin Device as Part of the Manual Methodology With and Without Shaking
The application note describes a comparative analysis of plasma protein binding results for free/bound drugs using two different sample extraction steps, with and without agitation.
Dry Granulation in Solid Oral Formulation: Advantages of Spray-Dried Mannitol in Roll Compaction
In recent years, mannitol has gained popularity as an excipient in solid dosage formulation due to its beneficial physicochemical properties.
Solid Formulation Development Using Melt-based 3D Printing Technologies
3D printing can overcome challenges during formulation development, with a focus on enhancing API bioavailability in solid dispersions. Find out how in this technical article.
Tablet Manufacturing Technologies for Solid Drug Formulation
Learn about common tablet manufacturing technologies and the advantages and disadvantages of each.
Drospirenone Assay - USP Monograph
Determination of the progestin medication, Drospirenone, using Purospher® STAR RP-18e Hibar® HPLC column and meeting the system suitability criteria as described in the USP monograph.
Bioburden Control in Bioprocessing
Bioburden control in bioprocessing requires assessing the risks, mitigating the risks, and monitoring bioburden levels.
Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing
To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
Improving Fed-batch Yields in Upstream Bioprocessing
Our step-by-step guide to optimizing media and feed combinations for upstream bioprocessing.
What is Multi Column Chromatography?
Multi column chromatography has the benefit of using less resin, increasing productivity, and enabling continuous processing. Learn more in this technical article.
Tangential Flow Filtration in Downstream Bioprocessing
There are many options for tangential flow filtration (TFF) in downstream bioprocessing from multi-use to single-use TFF or batch TFF and single-pass TFF.
Improving API Solubility using API Processing
Instead of addressing API solubility during formulation, tackle it early during development. Read about the various methods for API processing.
Use of PVA to Overcome Challenges in Ophthalmic Formulations
PVA meets all the essential requirements to be used in ophthalmic preparations and offers important benefits compared to HPMC and CMC.
Single-Pass Tangential Flow Filtration Implementation and Scaling
Get practical guidance on using single-pass tangential flow filtration in your downstream process and scaling between capsules and cassettes.
Viscosity Reduction in High Concentration TFF
This overview describes how use of viscosity-reducing excipients can address viscosity challenges in high concentration tangential flow filtration (TFF) and improve process economics.
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