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Pharmacology and drug discovery research

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Drug transport assays in a 96-well system: reproducibility and correlation to human absorption
Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.
Inhibition of Nucleic Acid Synthesis by Antibiotics
Quinolones are a key group of antibiotics that interfere with DNA synthesis by inhibiting topoisomerase, most frequently topoisomerase II (DNA gyrase), an enzyme involved in DNA replication.
Combining Permeability and Solubility Analyses in a Single Workflow
This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to
Phase I Drug Metabolism
Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues, the primary and first pass site of metabolism occurs
Membrane Integrity Test for Lipid-PAMPA Artificial Membranes
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
ADCC Assay Using BioIVT Cryopreserved NK Cells
ADCC assay performance and reproducibility using ready-to-use, cryopreserved NK effector cells, A549 target cells expressing a luciferase reporter gene, and anti-EGFR antibody.
Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
The Role of Intestinal Efflux Transporters In Drug Absorption
We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.
Degrader Building Blocks for Targeted Protein Degradation
Protein Degrader Building Blocks are a collection of crosslinker-E3 ligand conjugates with a pendant functional group for covalent linkage to a target ligand.
Primary Cell Culture Basics
What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.
Protein Binding Determination - Comparison Study of Techniques & Devices
Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
Understand the impact of phosphorothioate internucleotide linkage addition to oligonucleotide chromatography profiles.
Antimicrobial Peptides
With bacterial resistance and emerging infectious diseases becoming potential threats to humans, ribosomally synthesized antimicrobial peptides have become a promising focus area in antibiotic research.
Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)
This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.
The Role of Liver Transporters in Drug-Drug Interactions
Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.
Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters and Toxicity
Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal
3D Organoid Culture: New In Vitro Models of Development and Disease
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods
This article shows a comparison of the quantitative viability bioassays MTT, alamarBlue™, and Guava® ViaCount®.
Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption
Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.
Unnatural Amino Acids for Peptide Synthesis
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Hit Discovery & Confirmation for Early Drug Discovery
Hit discovery and confirmation allows identification of molecules acting against the target. It requires compound screening assays, depending on the drug target.
3D Cell Culture Tools and Techniques
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models