Plant tissue culture
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Applications:Plant tissue culture
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Vitamin Mixtures – Plant Tissue Culture Protocol
Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table
Antibiotics in Plant Tissue Culture Protocol
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
Pathology Media – Plant Tissue Culture Protocol
Fungal and Bacterial Detection Media, Media Preparation and Storage, Media Composition Table.
Silver Thiosulfate – Plant Tissue Culture Protocol
Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate into 100 ml of
Gelling Agents – Plant Tissue Culture Protocol
Find instructions for preparing alginate, agargel, and phytagel agar used to solidify media for plant tissue culture. Also provided are criteria for selecting the appropriate product for your application.
Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field
Plant/Fungi DNA Isolation Kit Protocol
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
Explant Sterilization – Plant Tissue Culture Protocol
Surface sterilizing plant material is an important for plant tissue culture. Commonly used disinfectants for plant tissue culture and methods for disinfecting seeds are included.