Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Isolated from adult human heart ventricles, human cardiac fibroblasts (HCF) are used to study myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. They play a critical role in extracellular matrix maintenance, growth factor synthesis, and cytokine synthesis. This protocol outlines the
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room
Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.