Primary cell culture

Applied Filters:

Applications:Primary cell culture

Content Type:Protocol

Showing 1-30 of 43 results

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Human Cardiac Fibroblasts (HCF) Culture Protocol

Isolated from adult human heart ventricles, human cardiac fibroblasts (HCF) are used to study myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. They play a critical role in extracellular matrix maintenance, growth factor synthesis, and cytokine synthesis. This protocol outlines the

Human Dermal Fibroblasts (HDF) Culture Protocol

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Lung Fibroblasts (HLF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Mononuclear Cells – Periphery Blood (HMNC-PB) Culture Protocol

Technical information for working with human mononuclear cells – periphery blood including thawing, subculturing and cryopreservation

Canine Aortic Endothelial Cells (CnAOEC) Culture Protocol

Technical information for working with Canine Aortic Endothelial Cells including thawing, subculturing and cryopreservation

Porcine Aortic Endothelial Cells (PAOEC) Culture Protocol

Technical information for working with porcine aortic endothelial cells including thawing, subculturing and cryopreservation

Human Dermal Microvascular Endothelial Cells (CADMEC™/HMVEC) Culture Protocol

Technical information for working with human dermal microvascular endothelial cells including thawing, subculturing and cryopreservation

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation

Rat Aortic Endothelial Cells (RAOEC) Culture Protocol

Technical information for working with rabbit aortic endothelial cells including thawing, subculturing and cryopreservation

Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol

Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol

Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation

Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Epidermal Melanocytes (HEM) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Mammary Epithelial Cells (HMEpC) Culture Protocol

Mammary Epithelial Cells and primary epithelial cells from other tissue at Cell Applications, your worldwide provider of human & animal cells, antibodies.

Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol

Technical information for working with human lung microvascular endothelial cells including thawing, subculturing and cryopreservation

Human Chondrocytes (HC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells

Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Page 1 of 2