Protein lysis and extraction
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Applications:Protein lysis and extraction
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Nuclear Protein Extraction Without the Use of Detergent
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins.
cOmplete™, Mini, EDTA-free Protocol
Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Lysosome Isolation Kit
Lysosomes are organelles ubiquitously distributed in most pro- and eukaryotic cells. The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation.
TRI Reagent® Protocol
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Liberase™ DH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Liberase™ DL Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend. Select the Liberase Enzyme Blend most similar to your tissue dissociation application, or