Protein purification

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Applications:Protein purification

Content Type:Protocol

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Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Packing Tricorn 10/100 Columns with MabSelect™ or MabSelect™ SuRe

This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G GraviTrap from Cytiva.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Affinity Purification with Ultrafree^®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.

Performing a Purification of Antibody Fragments with Capto™ L Products

This page shows how to purify antibody fragments by afﬁnity chromatography using Capto L products from Cytiva.

Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF

This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

Puriﬁcation of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Cell Disruption and Membrane Preparation

This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.

Performing a Separation with Sephacryl®

This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Refolding of proteins using gel ﬁltration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Column Packing and Preparation

This page describes efﬁcient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

Removal of Albumin Using Blue Sepharose® Chromatography Media

Remove albumin from afﬁnity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.

Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Puriﬁcation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of

Fractional Precipitation Protocol for Affinity Chromatography Samples

This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Column Packing and Preparation for Size Exclusion Chromatography

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