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LC/MS Analysis of Phospholipids in Milk on Ascentis® Express HILIC after SPE using Supelclean Si, Shotgun HPLC Analysis
(soybean), ≥97%; L-α-Phosphatidylethanolamine from egg yolk, Type III, ≥97% (TLC), lyophilized powder; L-α-Phosphatidylcholine, from egg yolk, Type XVI-E, ≥99% (TLC), lyophilized powder; Sphingomyelin, from chicken egg yolk, ≥95%
Banned and Other Neonicotinoids from Plant Material
On Friday, April 27, 2018, the European Union decided to ban the use of three neonicotinoid insecticides from use on field crops, having deemed them dangerous to bees. This application demonstrates the analysis of these banned compounds and others from
HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide
Separation of Acetylsalicylic acid, analytical standard; Salicylic acid, BioXtra, ≥99.0%
Lopinavir European Pharmacopeia HPLC Assay Method
This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method
LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5
LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5
HPLC Analysis of Organic Acids Commonly Found in Wine and Beer on TSKgel® OApak-P and OApak-A
Separation of Pyruvic acid, United States Pharmacopeia (USP) Reference Standard; Tartaric acid, United States Pharmacopeia (USP) Reference Standard; Citric acid, United States Pharmacopeia (USP) Reference Standard; Malic acid, United States Pharmacopeia (USP) Reference Standard; L-Pyroglutamic acid, ≥99.0% (T); Lactic acid
HPLC Analysis of Carotene Compounds on Ascentis® RP-Amide
HPLC Analysis of Carotene Compounds on Ascentis® RP-Amide
HPLC Analysis of Heroin, Cocaine, Amphetamine and MDMA on Ascentis® Express HILIC
Separation of Heroin, analytical standard, ≥98% (HPLC); Cocaine hydrochloride, analytical standard; D-Amphetamine hemisulfate salt solution, 1.0 mg/mL in methanol, analytical standard, for drug analysis; (±)-3,4-Methylenedioxymethamphetamine hydrochloride solution, analytical standard, for drug analysis, 1.0 mg/mL in methanol
LC/MS Analysis of L-Methionine Sulfoximine, Sulfone and Sulfoxide on Ascentis® Express HILIC
Separation of L-Methionine sulfone; L-Methionine sulfoximine; L-Methionine sulfoxide
Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions
To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.
HPLC Analysis of Fatty Acid Methyl Esters (FAMES) on SUPELCOSIL LC-18
Separation of Methyl decanoate; Methyl dodecanoate; Methyl myristate; Methyl palmitate; Methyl caprylate; Methyl oleate; Methyl linoleate; Methyl linolenate; Methyl stearate
LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R
Separation of L-(+)-Lactic acid, analytical standard; D-(−)-Lactic acid, 93-107%
Chloroquine Phosphate HPLC Assay and Impurity Profiling Methods Following the USP Monograph
Under applied conditions, system suitability criteria are met, and the Chloroquine Phosphate HPLC Assay and Impurity Profiling Methods demonstrate good resolution/selectivity, reproducibility, and sensitivity.
Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
Identification, Assay and Organic Impurity Profiling Methods for Aripiprazole following the United States Pharmacopeia Monograph
Aripiprazole is an atypical antipsychotic and a partial dopamine agonist. It is primarily used in the treatment of schizophrenia, bipolar disorder, major depressive disorder, tic disorders, and irritability associated with autism. Aripiprazole was first approved by the U.S. Food and
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases
Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases
HPLC Analysis of Furans on Ascentis® Express C18
HPLC Analysis of Furans on Ascentis® Express C18
Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography
Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography
Determination of Vitamin A and E Using HPLC with UV Detection Following the Chinese National Standard (GB) Method
Following GB method for Vitamin A and Vitamin E as it is written with a 250 x 4.6 mm Ascentis® Express C30 column gives baseline resolution between the critical pair (gamma-, and beta-tocopherol; peaks 3 and 4).
Chiral LC-MS Analysis of Methamphetamine in Urine on Astec® CHIROBIOTIC® V2
Efficient LC-MS separation of methamphetamine enantiomers in urine allows sensitive differentiation of illicit D-methamphetamine from over-the-counter L-methamphetamine.
HPLC Analysis of Vitamins B1 B6 B12
HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide
Enantiomeric Purities of Pharmaceuticals Using Carbohydrate-based Isothiocyanates
Enantiomeric analysis of neurotransmitters and pharmaceuticals carrying functional amino groups, ß-adrenergic blockers; penicillamine, mexiletine; fine chemicals such as 1-phenyl-2-aminoethanol.
Determination of Rutin in Anti-Aging Skin Cream Formulations Using Chromolith® HighResolution RP-18 Endcapped Column and UV Detection
This application illustrate how Rutin can be determined in skin cream samples using a monolithic Chromolith® HighResolution RP-18 endcapped column with UV detection.
HPLC Analysis of GMP, GDP and GTP on SeQuant ZIC-HILIC
HPLC Analysis of GMP, GDP and GTP on SeQuant ZIC-HILIC
HPLC Analysis of Fexofenadine and Related Compound B on CYCLOBOND® I 2000 Phases
The use of alternative chiral stationary phases to CYCLOBOND I 2000 SP suggested by USP, was studied for the analysis of fexofenadine and related compound B.
HPLC Analysis of Flavonoids on Ascentis® RP-Amide
Protocol for HPLC Analysis of Flavonoids on Ascentis® RP-Amide
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