Small molecule HPLC

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Applications:Small molecule HPLC

Content Type:Technical Article

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Assay and Organic Impurity Profiling of Apixaban Using an Ascentis^® Express C18 Column and UV Detection

Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core

Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection

Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus

HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant ZIC-HILIC

Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Cannabinoid Certified Reference Materials (CRMs) for an Improved Testing Accuracy and Traceability

We offer a portfolio of cannabinoid CRMs such as THC and THCA, available as single component solutions or mixes, certified in accordance with ISO 17034 and ISO/IEC 17025 for accurate cannabinoid profiling and potency testing.

Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples

Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples

SupraSolv® GC Solvent Specifications

Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.

Supel™-Select Polymeric SPE

Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

Bioanalysis with SPME

Biocompatible SPME devices employ C18 bonded porous silica sorbent particles, in a binder which resists fouling by biological matrix components

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Extraction and quantitative analysis of aminoglycosides in porcine tissue, using molecular imprinted polymer solid phase extraction followed by LC-MS/MS.

UHPLC Analysis of Conjugated Estrogens on Titan™ C18

The Titan C18 column provided efficient and rapid resolution of thirteen related estrogenic compounds. Ultra Ultra high purity solvents provided robust operation.

Determination of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS

This study is aimed at developing and validating an LC-ESI-ion trap-MS/MS method for identification and quantification of ethyl glucuronide (EtG) and ethyl sulfate (EtS) as ethanol biomarkers for urine analysis.

Determination of Buprenorphine and Norbuprenorphine in Urine Using LC-MS/MS

HPLC Products: The Best Choice for Any LC Instrument

Discover our comprehensive portfolio of High-performance liquid chromatography (HPLC) columns and consumables for your various and specialized U/HPLC based applications― 100% compatible with HPLC, UHPLC, or UPLC® systems from any vendor.

Ion Chromatography & Cation Suppression Straightforward Cation Analysis at Trace Levels

Ammonium determination in acidic solutions with Metrosep C Supp 2 column using the acidic binary IC eluent nitric acid/rubidium nitrate concentrate and the sodium bicarbonate/sodium carbonate suppressor regenerant.

Separation of Organic Acids with 100% aqueous mobile phase using an Ascentis^® Express AQ-C18 HPLC Column

A new RP-HPLC method is proposed for the determination of organic acids in 100% aqueous mobile phase using an Ascentis® Express AQ-C18 column

Improved Recoveries and Lower Background for GC/MS/MS and LC/MS/MS Analysis of Pesticides in Green Tea

A procedure for cleanup of green tea extracts provides low background and allowed for the analysis of more pesticides at lower levels than QuEChERS. Uses less solvents and no toluene.

Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

HPLC Troubleshooting Guide

Identify and correct common HPLC problems to restore column performance. Get help with peak problems (peak splitting, peak tailing, peak fronting, etc.), retention time, purge noise, and loss of resolution with example chromatograms.

HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions)

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates

Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.

UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR

UHPLC analysis of Benzoyl peroxide and degradation products on Purospher®STAR RP-18e Hibar® HR - Conditions & Description

HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, consumer products and as environmental pollutants dictates the need for a reliable analytical method. Ascentis HPLC columns, by virtue

Application Note – Peroxidase in Vegetables

Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.

UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns

Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.

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