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Alcian Blue - H&E - Metanil Yellow Stain for Diagnosing Barrett's Esophagus

Alcian Blue - H&E - Metanil Yellow Stain for Diagnosing Barrett's Esophagus. Barrett's esophagus is a condition that can lead to adenocarcinoma.

US EPA Method 8270 (PAH only): GC Analysis of PAHs on SLB®-5ms

GC Analysis of PAHs on SLB®-5ms

Packing a Column and Performing a Separation with Sephadex® LH-20

This page shows how to perform a separation with a Sephadex LH-20 column from Cytiva which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Assay Procedure for Cholesterol Esterase

Protocol for Assay Procedure for Cholesterol Esterase

Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol

Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Residual Analysis Optimized for Static Headspace GC Applications

Static headspace GC (GC-SH) is a technique used to concentrate volatile analytes prior to analysis. It can improve detection of low level volatile analytes and minimizes matrix interference by eliminating the need to inject the sample directly.

Removal of Detergents from Biological Product Matrices

The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.

Selecting a GC Column for Specific Methods

We offer the Supelco portfolio of analytical GC columns, providing you the accuracy, precision and consistency you need.

GC Analysis of Sweet Orange Essential Oil on SLB®-5ms (10 m x 0.10 mm I.D., 0.10 µm), Fast GC Analysis

-β-Farnesene; α-Huµlene; Germacrene D; (+)-Valencene; Bicyclogermacrene; (+)-δ-Cadinene

GC Analysis of Nitrogen and Nitrous Oxide on Carboxen®-1010 PLOT

Protocol for GC Analysis of Nitrogen and Nitrous Oxide on Carboxen®-1010 PLOT

Suitability for the qualitative determination of calcium with phthalein purple (O-cresophthalein complexone) using stannous chloride

Suitability for the Qualitative Determination of Calcium with Phthalein Purple (O-Cresophthalein Complexone) using Stannous Chloride

Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET-Biodiesel Capillary Column

Analytical Method: Aluminium (total) in effluents

Photometric determination with Chromazurole S subsequent to acid mineralisation; DIN decomposition

GC Analysis of FAMEs in Five Edible Oils (Canola, Lard, Palm, Soybean, and Sunflower Seed) on SLB^®-IL111

GC Analysis of FAMEs in Five Edible Oils (Canola, Lard, Palm, Soybean, and Sunflower Seed) on SLB®-IL111

US EPA Method 8015 (modified): GC Analysis of Diesel Range Organics (DRO) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 µm), Fast GC Analysis

Separation of Decane; Dodecane; Tetradecane; Hexadecane; Octadecane; Eicosane; Docosane; Tetracosane; Hexacosane; Octacosane

GC Analyses of Free Fatty Acids

This article will discuss the analysis of free fatty acid.

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Measuring Flows For Gas Chromatography – Volumetric vs. Mass

When choosing a flowmeter, it is essential to consider the attributes and needs of the application. This article provides information related to measuring GC flow rate by both volume and mass.

GC Analysis of Water (100%Standard) on Watercol™ 1460

Separation of Water

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear ﬂow and volumetric ﬂow rates in affinity chromatography.

Choosing the Proper Activated Alumina PLOT Column

The recent addition of Alumina sulfate and Alumina chloride columns to the Supelco family of GC capillary PLOT columns has prompted several customer inquiries on why we offer two alumina chemistries and how to chose between the two. We present

Millicup™-FLEX Disposable Vacuum Filtration Units and demo video

Millicup™-FLEX disposable vacuum filter was designed to reduce contamination and save time. The solvent-resistant unit allows for filtration into storage bottles.

GC Analysis of Permanent Gases and Light Hydrocarbons on Carboxen® -1006 PLOT

Protocol for GC Analysis of Permanent Gases and Light Hydrocarbons on Carboxen® -1006 PLOT

GC Analysis of Permanent Gases and Light Hydrocarbons on Supel-Q™ PLOT

Separation of Acetylene; Propyne; Propylene; Propane

Enzymatic Hydroxyproline Assay Protocol

Assay protocol for the colorimetric detection of Hydroxyproline in biological samples using the Hydroxypoline assay kit.

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

GC Analysis of Xylene Isomers on SLB®-IL60

GC Analysis of Xylene Isomers on SLB®-IL60 - The cresol (methylphenol) isomers are also precursors to many chemicals. This chromatogram of a mix of aromatic and methylphenol compounds was generated using an SLB-IL60 ionic liquid column. Its interaction mechanisms allow

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