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Showing 1-30 of 40 results for "G100120" within Papers
R H Coleman et al.
Current microbiology, 32(3), 147-150 (1996-03-01)
Pseudomonas syringae isolate BR2R produces tabtoxin, a beta-lactam-containing antibiotic, and the causative agent of wildfire disease of green bean (Phaseolus vulgaris). beta-Lactamase production has been suggested as the mechanism that protects P. syringae from tabtoxin. We sought to determine whether
Aisha Siddiqua et al.
Biological & pharmaceutical bulletin, 31(5), 802-808 (2008-05-03)
Low molecular weight acid phosphatase (LM-ACP) peak 2 (the isoenzyme corresponding to isoform 2, IF-2) from the liver of fish Rahu (Labeo rohita) was purified to homogeneity. 900 times purification resulted with specific activity of 35 U/mg of protein and
Serdar Güvercin et al.
Protein and peptide letters, 15(1), 6-12 (2008-01-29)
Glutathione S-transferase was purified from bovine erythrocytes and some kinetic and characteristic properties of the enzyme were investigated. The purification procedure was composed of preparation of homogenate and Glutathione-Agarose affinity chromatography. Thanks to the procedure, the enzyme was purified 6,800
Andre Müller et al.
The FEBS journal, 274(24), 6329-6339 (2007-11-22)
cDNA for octopine dehydrogenase (ODH) from the adductor muscle of the great scallop, Pecten maximus, was cloned using 5'- and 3'-RACE. The cDNA comprises an ORF of 1197 nucleotides and the deduced amino acid sequence encodes a protein of 399
Anna Gerdova et al.
Chirality, 23(7), 574-579 (2011-06-29)
There are well established theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, these have not been directly tested experimentally. We describe a test system with the chiral tris(ethylenediamine)Co(III) complex dissolved in water
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Production, purification and characterization of an a-amylase produced by <I>Saccharomycopsis fibuligera</I>
Gogoi, B.K., et al.
Journal of Applied Microbiology, 63(5), 373-379 (1987)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Dae-Young Lee et al.
Journal of medicinal food, 15(4), 399-405 (2012-02-10)
Grains of sugary rice were extracted with 80% aqueous methanol, and the concentrated extracts were successively partitioned using ethyl acetate, n-butanol, and water. From the n-butanol fractions, four flavonoid glycosides were isolated through repeated silica gel, octadecyl silica gel, and
Yun Chen et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(3), 362-365 (2013-05-15)
To determine the chemical constituents of Gentiana rhodantha. To isolate the constituents, column chromatography over silica gel, MCI, Sephadex LH-20 and C18 reverse-phased silica gel were used. Spectroscopic methods were used to elucidate the structures of the isolated compounds. Sixteen
M M Satorre et al.
Theriogenology, 78(7), 1548-1556 (2012-08-29)
The objectives were to evaluate postthaw sperm quality and the response to an inducer of in vitro sperm capacitation in boar sperm, cryopreserved with (T) or without (C) α-tocopherol. Boar sperm frozen in 0.2-mL pellets were thawed and washed (W)
Lila Boulekbache-Makhlouf et al.
Phytochemical analysis : PCA, 24(2), 162-170 (2012-08-30)
Eucalyptus species are widely cultivated in Mediterranean regions. Moreover, plants of this family have been utilized for medicinal purposes. A number of studies have been devoted to the identification of eucalypt phenolics, all of them have focused on specific families
Veronica M Chiu et al.
Analytical biochemistry, 418(1), 58-65 (2011-08-02)
An unstirred layer (USL) exists at the interface of solids with solutions. Thus, the particles in brain tissue preparations possess a USL as well as at the surface of a rotating disk electrode (RDE) used to measure chemical fluxes. Time
Olga N Solovjeva et al.
The protein journal, 31(2), 137-140 (2012-01-03)
Catalytic activity has been demonstrated for holotransketolase in the absence of free bivalent cations in the medium. The two active centers of the enzyme are equivalent in both the catalytic activity and the affinity for the substrates. In the presence
Marcílio S S Cunha-Filho et al.
The Journal of pharmacy and pharmacology, 63(9), 1156-1160 (2011-08-11)
The purpose of this work was to study the chemical stability of the new antitumoral β-lapachone (βLAP) to determine the degradation pathway/s of the molecule and the degradation kinetics in addition to identifying several degradation products. Samples of βLAP in
Yong Ding et al.
Journal of chromatography. A, 1218(47), 8503-8510 (2011-10-26)
Refolding enables bioprocesses predicated on proteins expressed as inclusion bodies in Escherichia coli. Optimization of size-exclusion chromatography (SEC) refolding is a significant challenge because a wide range of factors, including the choice of gel media, the column dimensions and configuration
Fat metabolism in higher plants. XLIV. Fatty acid synthesis by a soluble fatty acid synthetase from Sclanum tuberosum.
K P Huang et al.
Archives of biochemistry and biophysics, 143(2), 412-427 (1971-04-01)
Yong-Liang Yu et al.
Talanta, 88, 352-357 (2012-01-24)
A miniature analytical system based on a lab-on-valve platform is developed for trace metal analysis by bead injection spectroscopy. A multipurpose flow cell integrated into a lab-on-valve is furnished with two pieces of fiber optics to communicate with light source
Heng-chun Ren et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(17), 2581-2584 (2012-12-15)
To investigate chemical constituents from folk herb Pilea cavaleriei subsp. cavaleriei. The compounds were separated and purified by silica gel, Sephadex LH-20 and the like. The structures were identified by spectral methods such as (1)H, (13)C-NMR and MS. Seventeen compounds
Qiang Luo et al.
Journal of medicinal food, 14(12), 1609-1616 (2011-09-01)
Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE-cellulose 52 and Sephadex G-100 column chromatography, yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography
Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles
Pittet MJ, et al.
Nature Protocols, 1(1), 73-73 (2006)
Ahmed K A El-Sayed et al.
International journal of biological macromolecules, 132, 1274-1281 (2019-04-07)
AmyLa α-amylase gene from Laceyella sp. DS3 was heterologously expressed in E. coli BL21. E. coli BL21 maximally expressed AmyLa after 4 h of adding 0.02 mM IPTG at 37 °C. The recombinant AmyLa α-amylase was purified 2.19-fold through gel filtration and ion
Xiao-Yu Tao et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(24), 3738-3742 (2013-05-01)
The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on
Mohamed Ben Mansour et al.
Thrombosis research, 123(4), 671-678 (2008-07-12)
The polysaccharide fraction from the skin of the ray Raja radula was extracted, characterized and assayed for anticoagulant activity. A whole polysaccharidic fraction was extracted from the skin of the ray Raja radula by papain digestion followed by cetylpyridinium chloride
Qing-Qing Wang et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(3), 366-370 (2013-05-15)
To study the chemical constituents of Rhododendron seniavinii. Compounds were isolated from the aqueous extract of the leaves of R. seniavinii by using Sephadex LH-20, ODS open column chromatography and other means. Their structures were elucidated according to spectral data
Ebru Akkemik et al.
Environmental toxicology and pharmacology, 34(3), 888-894 (2012-09-20)
The glutathione S-transferases (EC 2.5.1.18) were purified and characterized from turkey liver for the first time. The enzyme was purified 252.7-fold with a yield of 45%, with a specific activity of 164.31 U/mg from turkey liver. The purity of the
Li-Ming Shen et al.
Talanta, 85(3), 1285-1290 (2011-08-03)
A flow-through fluorescence optosensor with Sephadex G-50 microbeads as solid support is developed for the sensitive determination of tetracycline (TC). The fluorescent TC derivative encapsulated in CTAB micelle structures is retained onto the surface of the microbeads packed into a
Molecular Basis of the Difference in Oxygen Affinity Between Maternal and Foetal Red Blood Cells in the Viviparous Garter Snake <I>Thanmophis Elegans</I>
Berner, N.J., and Ingermann, R.L.
The Journal of Experimental Biology, 140, 147-153 (1988)
Sanjiv K Mishra et al.
Protein expression and purification, 80(2), 234-238 (2011-07-13)
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
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