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Showing 1-30 of 52 results for "G6532" within Papers
Jens Hör et al.
Nucleic acids research, 48(16), 9301-9319 (2020-08-20)
Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes
Rosa Morra et al.
mBio, 9(1) (2018-02-01)
The apparent mislocalization or excretion of cytoplasmic proteins is a commonly observed phenomenon in both bacteria and eukaryotes. However, reports on the mechanistic basis and the cellular function of this so-called "nonclassical protein secretion" are limited. Here we report that
Abdalla Al Refaii et al.
Molecular microbiology, 71(3), 748-762 (2008-12-05)
In Escherichia coli strains carrying null mutations in either the dnaK or dnaJ genes, the late stages of 30S and 50S ribosomal subunit biogenesis are slowed down in a temperature-dependent manner. At high temperature (44 degrees C), 32S and 45S
Masatoshi Miyakoshi et al.
eLife, 11 (2022-11-29)
Glutamine synthetase (GS) is the key enzyme of nitrogen assimilation induced under nitrogen limiting conditions. The carbon skeleton of glutamate and glutamine, 2-oxoglutarate, is supplied from the TCA cycle, but how this metabolic flow is controlled in response to nitrogen
Gianluca Matera et al.
Molecular cell, 82(3), 629-644 (2022-01-23)
The envelope of Gram-negative bacteria is a vital barrier that must balance protection and nutrient uptake. Small RNAs are crucial regulators of the envelope composition and function. Here, using RIL-seq to capture the Hfq-mediated RNA-RNA interactome in Salmonella enterica, we
Chaperonin GroESL mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli.
Lee KH, et al.
Biochemical and Biophysical Research Communications, 298(2), 216-224 (2002)
Clivia Lisowski et al.
Autophagy, 18(8), 1785-1800 (2021-11-17)
Modulation of the host cell cycle has emerged as a common theme among the pathways regulated by bacterial pathogens, arguably to promote host cell colonization. However, in most cases the exact benefit ensuing from such interference to the infection process
Miryoung Song et al.
Nature communications, 8, 14737-14737 (2017-05-10)
Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the
Sandy R Pernitzsch et al.
Nature communications, 12(1), 4433-4433 (2021-07-23)
The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase
Tetracycline treatment and sex-ratio distortion: a role for Wolbachia in the moulting of filarial nematodes?
Casiraghi M, et al.
International Journal For Parasitology, 32(12), 1457-1468 (2002)
Sahil Sharma et al.
Methods in molecular biology (Clifton, N.J.), 2404, 111-133 (2021-10-26)
CRISPR-Cas systems consist of a complex ribonucleoprotein (RNP) machinery encoded in prokaryotic genomes to confer adaptive immunity against foreign mobile genetic elements. Of these, especially the class 2, Type II CRISPR-Cas9 RNA-guided systems with single protein effector modules have recently
Irina V Mikheyeva et al.
Nature communications, 14(1), 5715-5715 (2023-09-16)
The outer membrane (OM) of Gram-negative bacteria such as Escherichia coli is an asymmetric bilayer with the glycolipid lipopolysaccharide (LPS) in the outer leaflet and glycerophospholipids in the inner. Nearly all integral OM proteins (OMPs) have a characteristic β-barrel fold
Sara K Eisenbart et al.
Molecular cell, 80(2), 210-226 (2020-10-02)
Many bacterial pathogens regulate their virulence genes via phase variation, whereby length-variable simple sequence repeats control the transcription or coding potential of those genes. Here, we have exploited this relationship between DNA structure and physiological function to discover a globally
Gitte M Knudsen et al.
Environmental microbiology, 14(8), 1855-1875 (2012-02-24)
Although the growth of bacteria has been studied for more than a century, it is only in recent decades that surface-associated growth has received attention. In addition to the well-characterized biofilm and swarming lifestyles, bacteria can also develop as micro-colonies
Sara Correia Santos et al.
Cell reports, 34(5), 108722-108722 (2021-02-04)
A full understanding of the contribution of small RNAs (sRNAs) to bacterial virulence demands knowledge of their target suites under infection-relevant conditions. Here, we take an integrative approach to capturing targets of the Hfq-associated sRNA PinT, a known post-transcriptional timer
Selma Metaane et al.
PloS one, 18(9), e0291736-e0291736 (2023-09-15)
In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σS/RpoS, remodels global gene expression to reshape the physiology of stationary phase cells and ensure their survival under non-optimal growth conditions. In the foodborne pathogen Salmonella enterica serovar Typhimurium
Xiang Wu et al.
BMC microbiology, 11, 87-87 (2011-04-30)
The periplasmic High Temperature Requirement protein A (HtrA) plays important roles in bacterial protein folding and stress responses. However, the role of chlamydial HtrA (cHtrA) in chlamydial pathogenesis is not clear. The cHtrA was detected both inside and outside the
Jeffrey K Duncan-Lowey et al.
Cell reports, 33(8), 108409-108409 (2020-11-26)
During infection, some bacterial pathogens invade the eukaryotic cytosol and spread between cells of an epithelial monolayer. Intercellular spread occurs when these pathogens push against the plasma membrane, forming protrusions that are engulfed by adjacent cells. Here, we show that
Milan Gerovac et al.
RNA (New York, N.Y.), 26(10), 1448-1463 (2020-07-11)
RNA-binding proteins (RBPs) play important roles in bacterial gene expression and physiology but their true number and functional scope remain little understood even in model microbes. To advance global RBP discovery in bacteria, we here establish glycerol gradient sedimentation with
Conor J McGrath et al.
Disease models & mechanisms, 15(4) (2022-03-19)
The gut microbiota plays a crucial role in protecting against enteric infection. However, the underlying mechanisms are largely unknown owing to a lack of suitable experimental models. Although most gut commensals are anaerobic, intestinal epithelial cells require oxygen for survival.
Alexandre Smirnov et al.
The EMBO journal, 36(8), 1029-1045 (2017-03-25)
Research into post-transcriptional control of mRNAs by small noncoding RNAs (sRNAs) in the model bacteria
May N Taw et al.
ACS synthetic biology, 10(11), 2947-2958 (2021-11-11)
Escherichia coli remains one of the preferred hosts for biotechnological protein production due to its robust growth in culture and ease of genetic manipulation. It is often desirable to export recombinant proteins into the periplasmic space for reasons related to
Keith D MacKenzie et al.
PLoS genetics, 15(6), e1008233-e1008233 (2019-06-25)
Pathogenic Salmonella strains that cause gastroenteritis are able to colonize and replicate within the intestines of multiple host species. In general, these strains have retained an ability to form the rdar morphotype, a resistant biofilm physiology hypothesized to be important
Esther García-Fernández et al.
Cell, 171(6), 1354-1367 (2017-11-07)
A number of bacterial cell processes are confined functional membrane microdomains (FMMs), structurally and functionally similar to lipid rafts of eukaryotic cells. How bacteria organize these intricate platforms and what their biological significance is remain important questions. Using the pathogen
Christopher J Rice et al.
PloS one, 10(6), e0127523-e0127523 (2015-06-04)
The expression of genes within Salmonella Pathogenicity Islands 1 and 2 (SPI1, SPI2) is required to facilitate invasion and intracellular replication respectively of S. Typhimurium in host cell lines. Control of their expression is complex and occurs via a variety
Mary S Morrison et al.
Nature communications, 12(1), 5959-5959 (2021-10-15)
The directed evolution of antibodies has yielded important research tools and human therapeutics. The dependence of many antibodies on disulfide bonds for stability has limited the application of continuous evolution technologies to antibodies and other disulfide-containing proteins. Here we describe
Nadja Heinz Ernst et al.
mBio, 9(3) (2018-06-21)
Numerous Gram-negative bacterial pathogens utilize type III secretion systems (T3SSs) to inject tens of effector proteins directly into the cytosol of host cells. Through interactions with cognate chaperones, type III effectors are defined and recruited to the sorting platform, a
Immunohistochemical/immunogold detection and distribution of the endosymbiont Wolbachia of Dirofilaria immitis and Brugia pahangi using a polyclonal antiserum raised against WSP (Wolbachia surface protein).
Kramer L, et al.
Parasitology Research, 89(5), 381-386 (2003)
Victor Parro et al.
Astrobiology, 11(10), 969-996 (2011-12-14)
The Atacama Desert has long been considered a good Mars analogue for testing instrumentation for planetary exploration, but very few data (if any) have been reported about the geomicrobiology of its salt-rich subsurface. We performed a Mars analogue drilling campaign
Kotaro Chihara et al.
RNA (New York, N.Y.) (2022-11-04)
New methods for the global identification of RNA-protein interactions have led to greater recognition of the abundance and importance of RNA-binding proteins (RBPs) in bacteria. Here, we expand this tool kit by developing SEC-seq, a method based on a similar
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