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Showing 1-20 of 20 results for "GE17-0120-01" within Papers
F H Kirkpatrick et al.
Electrophoresis, 14(4), 349-354 (1993-04-01)
Properties of agarose potentially relevant to PFGE (pulsed-field gel electrophoresis) are reviewed, and some new information is presented. Agarose polymers appear to have molecular weights in the range of 100,000 to 200,000 Da, but this is not tightly related to
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
Hu Liu et al.
Journal of chromatography. A, 1344, 59-65 (2014-04-29)
This work proposed an integrative method of protein refolding and purification by like-charged resin facilitated refolding and metal-chelate affinity adsorption. Hexahistidine-tagged enhanced green fluorescence protein (EGFP) was overexpressed in Escherichia coli as inclusion bodies (IBs), and then the protein was
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
P Upcroft et al.
Journal of chromatography, 618(1-2), 79-93 (1993-08-25)
Agarose as a medium for separation of DNA was first introduced in 1962 and since the early 1970s agarose submarine gel electrophoresis has been synonymous with separations of DNA molecules larger than 1 kilobase pair (kb). The large pore size
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
N C Stellwagen et al.
Electrophoresis, 14(4), 355-368 (1993-04-01)
The orientation of the agarose gel matrix in pulsed electric fields has been studied by transient electric birefringence. Two types of agarose with different degrees of charge were studied, in addition to agarose solutions and gels containing beta-carrageenan, a stereoisomer
Takayuki Takei et al.
Journal of bioscience and bioengineering, 117(4), 497-500 (2013-11-05)
Agarose gels were superior to calcium-alginate gels for immobilizing Rhodococcus erythropolis CS98 strain to remove cesium from water. Suitable incubation time of the immobilized cells in cesium solutions, cell number in the gels and volume ratio of the cesium solution
Y Tomita et al.
Ultrasonics, 55, 1-5 (2014-08-19)
Sonoporation has the potential to deliver extraneous molecules into a target tissue non-invasively. There have been numerous investigations of cell membrane permeabilization induced by microbubbles, but very few studies have been carried out to investigate sonoporation by inertial cavitation, especially
Sudhir Chandna et al.
International journal of radiation biology, 90(5), 401-406 (2014-02-18)
Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. Macrocolony formation
Dorota Kubacka et al.
Journal of molecular biology, 427(2), 387-405 (2014-12-03)
eIF4E1b, closely related to the canonical translation initiation factor 4E (eIF4E1a), cap-binding protein is highly expressed in mouse, Xenopus and zebrafish oocytes. We have previously characterized eIF4E1b as a component of the CPEB mRNP translation repressor complex along with the
Donghyeon Kim et al.
Brain stimulation, 8(5), 914-925 (2015-07-26)
Although computational studies of electrical brain stimulation (EBS) have received attention as a cost-effective tool, few studies have validated the technique, particularly in invasive cortical stimulation. In order to validate such studies, we used EBS to compare electric potential distributions
Santoshkumar Biradar et al.
Journal of nanoscience and nanotechnology, 14(6), 4257-4263 (2014-04-18)
In this study, a novel approach to tailor the calcium carbonate nanoparticles was exploited based on agarose gel as polymer medium. The size of nanoparticles formed was governed by ionic diffusion and affected by weight percent of agarose and reaction
Andrea Trochine et al.
Experimental parasitology, 140, 33-38 (2014-03-19)
Benznidazole (Bzn) is a nitroimidazole drug currently used as first line treatment against Chagas disease, a neglected tropical disease caused by the flagellated protozoan Trypanosoma cruzi. Although the drug has been used since the late 1960s, its mechanism of action
Yan Hong et al.
Journal of chromatography. A, 1342, 30-36 (2014-04-02)
Previously, we studied bovine serum albumin (BSA) uptake to poly(ethylenimine) (PEI)-grafted Sepharose resins, and an ionic capacity (IC) range (600-740mmol/L) for steep increases of both protein capacity (qm) and effective pore diffusion coefficient (De) was found. In this work, seven
The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Sieving by agarose gels and its use during pulsed-field electrophoresis.
P Serwer
Biotechnology & genetic engineering reviews, 8, 319-343 (1990-01-01)
Shuhei Konagaya et al.
Biochimica et biophysica acta, 1850(1), 22-32 (2014-10-05)
Dopamine neurons derived from induced pluripotent stem cells have been widely studied for the treatment of Parkinson's disease. However, various difficulties remain to be overcome, such as tumor formation, fragility of dopamine neurons, difficulty in handling large numbers of dopamine
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds
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