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General Instructions for Affinity Purification Using HiTrap® Columns
General instructions for affinity purification using HiTrap columns from Cytiva.
Protein A-Based Separation with Cytiva Products
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Sample Preparation for Affinity Chromatography
This page shows how to prepare samples for purification with affinity chromatography.