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Separation with Cytiva Products
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.