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Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
Purification using GST SpinTrap™
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
General Considerations for Purification of GST-tagged Proteins
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
Purification of GST-tagged Protein
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
Troubleshooting of Cleavage Methods
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.