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General Considerations for Purification of GST-tagged Protein
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
IgG Antibody Purification with HiTrap® Protein G HP Columns
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.