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Showing 1-30 of 42 results for "GE17117701SIGMA" within Site Content
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Conditioning Purified Membrane Proteins
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
HIC Purification Options and Scale up with SOURCE™ Media
This page discusses how to do a purification with SOURCE™ media from Cytiva.
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Separation Options with Sephacryl®
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
Sephacryl® Separation
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Refolding: Ion Exchange Chromatography
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Reverse Phased Chromatography (RPC) in Practice
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
Salt Selection & Buffer Preparation
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
Hydrophobic Interaction Chromatography Setup
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
Sample Preparation for Chromatographic Purification
This page discusses various aspects of sample preparation for chromatographic purification.
Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography
This page discusses column packing and preparation techniques for reverse phase chromatography.
HIC as a Capture Step
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Performing an Isolation of Native Complexes
This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
IgG Antibody Purification with HiTrap® Protein G HP Columns
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Protein A-Based Separation with Cytiva Products
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Performing a Separation with Sephadex®
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
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